[1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998; 282:1145-7.10.1126/science.282.5391.1145]Open DOISearch in Google Scholar
[2. Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol. 2000; 2:271-8.10.1006/dbio.2000.9912]Open DOISearch in Google Scholar
[3. Itskovitz-Eldor J, Schuldiner M, Karsenti D, Eden A, Yanuka O, Amit M, et al. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med. 2000; 6:88-95.10.1007/BF03401776]Search in Google Scholar
[4. Wobus AM, Boheler KR. Embryonic stem cells: prospects for developmental biology and cell therapy. Physiol Rev. 2005; 85:635-78.10.1152/physrev.00054.2003]Open DOISearch in Google Scholar
[5. Mayor S. First patient enters trial to test safety of stem cells in spinal injury. BMJ. 2010; 12:c5724.10.1136/bmj.c5724]Open DOISearch in Google Scholar
[6. Aznar J, Sanchez JL. Embryonic stem cells: are useful in clinic treatments? J Physiol Biochem. 2011; 67: 141-4.10.1007/s13105-010-0064-0]Open DOISearch in Google Scholar
[7. Schwartz SD, Hubschman JP, Heilwell G, Franco- Cardenas V, Pan CK, Ostrick RM, et al. Embryonic stem cell trials for macular degeneration: a preliminary report. Lancet. 2012; 379:713-20.10.1016/S0140-6736(12)60028-2]Search in Google Scholar
[8. Pease S, Braghetta P, Gearing D, Grail D, Williams RS. Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 1990; 141:344-52.10.1016/0012-1606(90)90390-5]Search in Google Scholar
[9. Nagy A, Rossant J, Nagy R, Abramow-Newerly W, Roder JC. Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc Natl Acad Sci USA. 1993; 90:8424-8.10.1073/pnas.90.18.8424473698378314]Open DOISearch in Google Scholar
[10. Berger CN, Sturm KS. Self renewal of embryonic stem cells in the absence of feeder cells and exogenous leukaemia inhibitory factor. Growth Factors. 1997; 14: 145-59.10.3109/089771997090215179255606]Open DOISearch in Google Scholar
[11. Richards M, Fong CY, Chan WK, Wong PC, Bongso A. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol. 2002; 20:933-6.10.1038/nbt72612161760]Open DOISearch in Google Scholar
[12. Amit M, Margulets V, Sege H, Shariki K, Laevsky I, Coleman R, et al. Human feeder layers for human embryonic stem cells. Biol Reprod. 2003; 6:2150-6.10.1095/biolreprod.102.01258312606388]Search in Google Scholar
[13. Hovatta O, Mikkola M, Gertow K. A culture system using human foreskin fibroblasts as feeder cells allow production of human embryonic stem cells. Hum Reprod. 2003; 18:1404-9.10.1093/humrep/deg29012832363]Open DOISearch in Google Scholar
[14. Cheng L, Hammond H, Ye Z, Zhan X, Dravid G. Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. Stem Cells. 2003; 21:131-42.10.1634/stemcells.21-2-13112634409]Open DOISearch in Google Scholar
[15. Park Y, Lee SJ, Cho, IY, Lee SR, Sung HJ, Kim JH, et al. The efficacy of human placenta as a source of the universal feeder in human and mouse pluripotent stem cell culture. Cell Reprogram. 2010; 12:315-28.10.1089/cell.2009.011320698773]Open DOISearch in Google Scholar
[16. Zhang K, Cai Z, Li Y, Shu J, Pan L, Van F, et al. Utilization of human amniotic mesenchymal cells as feeder layers to sustain propagation of human embryonic stem cells in the undifferentiated state. Cell Reprogram. 2011; 13:281-8.10.1089/cell.2010.010321718108]Open DOISearch in Google Scholar
[17. Lee EJ, Kang HJ, Lee HN, Kang SK, Kim KH, Lee SW, et al. New culture system for human embryonic stem cells: autologous mesenchymal stem cell feeder without exogenous fibroblast growth factor 2. Differentiation. 2012; 83:92-100.10.1016/j.diff.2011.09.00322099180]Search in Google Scholar
[18. Braam SR, Denning C, Matsa E, Young LE, Passier R, Mummery CL. Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification. Nat Protoc. 2008; 9:1435-43.10.1038/nprot.2008.14018772870]Open DOISearch in Google Scholar
[19. Meng G, Liu S, Krawetz R, Chan M, Chernos J, Rancourt DE. A novel method for generating xenofree human feeder cells for human embryonic stem cell culture. Stem Cells Dev. 2008; 17:413-22.10.1089/scd.2007.023618513158]Open DOISearch in Google Scholar
[20. Amit M, Shariki C, Margulets V, Itskovitz-Eldor J. Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod. 2004, 70:837-845.10.1095/biolreprod.103.02114714627547]Open DOISearch in Google Scholar
[21. Bendall SC, Stewart MH, Menendez P, George D, Vijayaragavan K, Werbowetski-Ogilvie T, et al. IGF and FGF cooperatively establish the regulatory stem cell niche of pluripotent human cells in vitro. Nature. 2007; 448:1015-21.10.1038/nature0602717625568]Search in Google Scholar
[22. Lei T, Jacob S, Ajil-Zaraa I, Dubuisson JB, Irion O, Jaconi M, Feki A. Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges. Cell Res. 2007; 17:682-8.10.1038/cr.2007.6117667917]Open DOISearch in Google Scholar
[23. Swistowski A, Peng J, Han Y, Swistowska AM, Rao MS, Zeng X. Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them. PLoS One. 2009; 7:e6233.10.1371/journal.pone.0006233270518619597550]Open DOISearch in Google Scholar
[24. Hernandez D, Ruban L, Mason C. Feeder-free culture of human embryonic stem cells for scalable expansion in a reproducible manner. Stem Cells Dev. 2011; 20: 1089-98.10.1089/scd.2009.050721142495]Open DOISearch in Google Scholar
[25. Montes R, Ligero G, Sanchez L, Catalina P, de la Cueva T, Nieto A, et al. Feeder-free maintenance of hESCs in mesenchymal stem cell-conditioned media: distinct requirements for TGF-beta and IGF-II. Cell Res. 2009; 19:698-709.10.1038/cr.2009.3519308090]Open DOISearch in Google Scholar
[26. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 2000; 97:13625-30.10.1073/pnas.2403097971762611087820]Open DOISearch in Google Scholar
[27. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA. 2003; 100:5807-12.10.1073/pnas.093763510015628212716973]Open DOISearch in Google Scholar
[28. Huang, AH, Chen YK, Lin LM, Shieh TY, Chan AW. Isolation and characterization of dental pulp stem cells from a supernumerary tooth. J Oral Pathol Med. 2008; 37:571-4.10.1111/j.1600-0714.2008.00654.x18331285]Open DOISearch in Google Scholar
[29. Karaoz E, Dogan BN, Aksoy A, Gacar G, Akyuz S, Ayhan S, et al. Isolation and in vitro characterisation of dental pulp stem cells from natal teeth. Histochem Cell Biol. 2010; 133:95-112.10.1007/s00418-009-0646-519816704]Open DOISearch in Google Scholar
[30. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, et al. Stem cell properties of human dental pulp stem cells. J Dent Res. 2002; 81:531-5.10.1177/15440591020810080612147742]Open DOISearch in Google Scholar
[31. Zhang W, Walboomers XF, Van Kuppevelt TH, Daamen WF, Van Damme PA, Bian Z, et al. In vivo evaluation of human dental pulp stem cells differentiated towards multiple lineages. J Tissue Eng Regen Med. 2008; 2: 117-25.10.1002/term.7118338838]Open DOISearch in Google Scholar
[32. Huang GT, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009; 88:792-806.10.1177/0022034509340867283048819767575]Open DOISearch in Google Scholar
[33. Struys T, Moreels M, Martens W, Donders R, Wolfs E, Lambrichts I. Ultrastructural and immunocytochemical analysis of multilineage differentiated human dental pulp- and umbilical cord-derived mesenchymal stem cells. Cells Tissues Organs. 2011; 193:366-78.10.1159/00032140021124001]Open DOISearch in Google Scholar
[34. Tamaki Y, Nakahara T, Ishikawa H, Sato S. In vitro analysis of mesenchymal stem cells derived from human teeth and bone marrow. Odontology. 2013; 101: 121-32.10.1007/s10266-012-0075-022772774]Open DOISearch in Google Scholar
[35. Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu TM, et al. Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use. Tissue Eng Part C Methods. 2008; 14:149-56.10.1089/ten.tec.2008.0031296362918489245]Open DOISearch in Google Scholar
[36. Eslaminejad MB, Vahabi S, Shariati M. In vitro growth and characterization of stem cells from human dental pulp of deciduous versus permanent teeth. J Dent (Tehran). 2010; 7:185-95.]Search in Google Scholar
[37. Pruksananonda K, Rungsiwiwut R, Numchaisrika P, Ahnonkitpanit V, Israsena N, Virutamasen P. Eighteen-year cryopreservation does not negatively affect the pluripotency of human embryos: evidence from embryonic stem cell derivation. Bioresearch Open Access. 2012: 1:166-73.10.1089/biores.2012.0242355920423514952]Search in Google Scholar
[38. Chattong S, Tanamai J, Kiatsomchai K, Nakatsu M, Sereemaspun A, Pimpha N, et al. Glutaraldehyde erythropoietin protects kidney in I/R injury without increased red blood cell production. Br J Pharm. 2013; 168:189-99.10.1111/j.1476-5381.2012.02123.x357001422861820]Search in Google Scholar
[39. Xiao L, Yuan X, Sharkis SJ. Activin A maintains selfrenewal and regulates fibroblast growth factor, Wnt, and bone morphogenic protein pathways in human embryonic stem cells. Stem Cells. 2006; 24:1476-86.10.1634/stemcells.2005-029916456129]Open DOISearch in Google Scholar
[40. Beattie GM, Lopez AD, Bucay N, Hinton A, Firpo MT, King CC, et al. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells. 2005; 23:489-95.10.1634/stemcells.2004-027915790770]Open DOISearch in Google Scholar
[41. Xu C, Jiang J, Sottile V, McWhir J, Lebkowski J, Carpenter MK. Immortalized fibroblast-like cells derived from human embryonic stem cells support undifferentiated cell growth. Stem Cells. 2004; 22: 972-80.10.1634/stemcells.22-6-97215536188]Open DOISearch in Google Scholar
[42. Liu J, Jin T, Chang S, Ritchie HH, Smith AJ, Clarkson BH. Matrix and TGF-β-related gene expression during human dental pulp stem cell (DPSC) mineralization. In Vitro Cell Dev Biol Anim. 2007; 43:120-8.10.1007/s11626-007-9022-8]Open DOISearch in Google Scholar
[43. Vanhelleputte P, Nijs K, Delforge M, Evers G, Vanderschueren S. Pain during bone marrow aspiration: prevalence and prevention. J Pain Symptom Manage. 2003; 26:860-6.10.1016/S0885-3924(03)00312-9]Open DOISearch in Google Scholar