<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Deiris reticulata Craib is a Thai medicinal plant in the Legiuninosae family. It has flavonoids as major active compounds similar to other plants in genus Denis. Several flavonoids have been identified to have anti-inflammatory activities. The anti-inflammatory mechanisms and potency of flavonoids from this medicinal plant is not known.Objective: We investigated the effect of ethanolic extract fr om stem of D. reticulata on LPS-induced macrophage activation.Methods: J774A.1 cells were treated with 6.25-100 g/ml the extract for 24 horns and then activated with 100 ng/ml lipopolysaccharide (LPS) for 24 horns. Tire extract inhibited nitric oxide production in LPS-activated J774A. 1 cells in concentration-dependent maimer with IC50 62.5 μg/ml. The effect of this extract on phagocytosis activity of LPS-activated J774A. 1 cells was also investigated.Results: The extract at concentrations of 50 and 100 pg/ml significantly inhibited zymosan phagocytosis of LPS-activated cells in a concentration dependent maimer. It decreased the mRNA expression of the inducible nitric oxide synthase (iNOS) which plays a role in NO production and the cyclo-oxygenase 2 (COX-2) which is responsible for prostaglandins (PGs) production in LPS-activated J774A. 1 cells. This extract also inhibited the mRNA expression of pro-inflammatory cytokines. TNF-α. IL-β and IL-6 in the LPS-activated J774A. 1 cells.Conclusion: The results hi this study reveal for the first time that the ethanolic extract of D. reticulata stem has potential anti-inflammatory activity. It inhibited production of several known inflammatory mediators in LPS-activated macrophages. These findings may be useful to study this plant further as a source of anti­inflammatory activity. </abstract>

Background: Deiris reticulata Craib is a Thai medicinal plant in the Legiuninosae family. It has flavonoids as major active compounds similar to other plants in genus Denis. Several flavonoids have been identified to have anti-inflammatory activities. The anti-inflammatory mechanisms and potency of flavonoids from this medicinal plant is not known.Objective: We investigated the effect of ethanolic extract fr om stem of D. reticulata on LPS-induced macrophage activation.Methods: J774A.1 cells were treated with 6.25-100 g/ml the extract for 24 horns and then activated with 100 ng/ml lipopolysaccharide (LPS) for 24 horns. Tire extract inhibited nitric oxide production in LPS-activated J774A. 1 cells in concentration-dependent maimer with IC50 62.5 μg/ml. The effect of this extract on phagocytosis activity of LPS-activated J774A. 1 cells was also investigated.Results: The extract at concentrations of 50 and 100 pg/ml significantly inhibited zymosan phagocytosis of LPS-activated cells in a concentration dependent maimer. It decreased the mRNA expression of the inducible nitric oxide synthase (iNOS) which plays a role in NO production and the cyclo-oxygenase 2 (COX-2) which is responsible for prostaglandins (PGs) production in LPS-activated J774A. 1 cells. This extract also inhibited the mRNA expression of pro-inflammatory cytokines. TNF-α. IL-β and IL-6 in the LPS-activated J774A. 1 cells.Conclusion: The results hi this study reveal for the first time that the ethanolic extract of D. reticulata stem has potential anti-inflammatory activity. It inhibited production of several known inflammatory mediators in LPS-activated macrophages. These findings may be useful to study this plant further as a source of anti­inflammatory activity.

Inhibitory effect of Derris reticulata ethanol extract on LPS-induced macrophage activation

Interdepartmental Program of Pharmacology, Graduate School Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330,

Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Deiris reticulata Craib is a Thai medicinal plant in the Legiuninosae family. It has flavonoids as major active compounds similar to other plants...

Inhibitory effect of Derris reticulata ethanol extract on LPS-induced macrophage activation

Article Category: Original article

Data publikacji: 04 lut 2017

Zakres stron: 89 - 95

DOI: https://doi.org/10.5372/1905-7415.0701.154

Słowa kluczowe
Activated macrophage, anti-inflammation, Derris reticulata, phagocytosis, pro-inflammatory cytokines, pro-inflammatory mediators

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Inhibitory effect of Derris reticulata ethanol extract on LPS-induced macrophage activation

Article Category: Original article

Data publikacji: 04 lut 2017

Zakres stron: 89 - 95

DOI: https://doi.org/10.5372/1905-7415.0701.154

Słowa kluczoweActivated macrophage, anti-inflammation, Derris reticulata, phagocytosis, pro-inflammatory cytokines, pro-inflammatory mediators

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Słowa kluczowe
Activated macrophage, anti-inflammation, Derris reticulata, phagocytosis, pro-inflammatory cytokines, pro-inflammatory mediators