<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Human plasma paraoxonase1 (PON1) is an esterase catalyzing the hydrolysis of organophosphorus pesticides and other xenobiotics. The aims of this study were to develop a rapid method to determinate PON1 activity, evaluate some interference, and study the influence of storage temperature on PON1 activity assay.Methods: Measurement of PON1 activity was performed for 369 samples by measuring the hydrolysis of paraoxon using a spectrophotometric method adapted on konelab 30 ⃞.Results: The developed method facilitates the determination of PON1 activity at the rate of more than 200 samples per hour, and it is linear between 2 and 900 IU/L. Intra and inter-assay imprecision coefficients of variation were 2% and 5% respectively. PON1 activity in serum was correlated with those in heparinized plasma (r = 0.994, p &lt; 0.001) and in plasma/EDTA (r = 0.962, p &lt; 0.001). The mean inhibition of the PON1 activity was, by EDTA/K3, 41 ± 10 %. There was not significant PON1 activity variation after 40 days of storage at -20°C or at +4 ⃞ C. There were no substantial interferences from haemoglobin, jaundice and hyperlipidemia.Conclusion: The developed method is reliable, reproducible, and suitable. It can also be performed on heparinized plasma for the determination of PON1 activity. Hence, it may be useful for assaying PON1 activity in several intoxications such as organophosphorus, sarin, and soman nerve agents.</abstract>

Background: Human plasma paraoxonase1 (PON1) is an esterase catalyzing the hydrolysis of organophosphorus pesticides and other xenobiotics. The aims of this study were to develop a rapid method to determinate PON1 activity, evaluate some interference, and study the influence of storage temperature on PON1 activity assay.Methods: Measurement of PON1 activity was performed for 369 samples by measuring the hydrolysis of paraoxon using a spectrophotometric method adapted on konelab 30 ⃞.Results: The developed method facilitates the determination of PON1 activity at the rate of more than 200 samples per hour, and it is linear between 2 and 900 IU/L. Intra and inter-assay imprecision coefficients of variation were 2% and 5% respectively. PON1 activity in serum was correlated with those in heparinized plasma (r = 0.994, p < 0.001) and in plasma/EDTA (r = 0.962, p < 0.001). The mean inhibition of the PON1 activity was, by EDTA/K3, 41 ± 10 %. There was not significant PON1 activity variation after 40 days of storage at -20°C or at +4 ⃞ C. There were no substantial interferences from haemoglobin, jaundice and hyperlipidemia.Conclusion: The developed method is reliable, reproducible, and suitable. It can also be performed on heparinized plasma for the determination of PON1 activity. Hence, it may be useful for assaying PON1 activity in several intoxications such as organophosphorus, sarin, and soman nerve agents.

Development of an automated method for the determination of human paraoxonase1 activity

Laboratory of Biochemistry, Faculty of Medicine of Monastir, University Hospital Fattouma Bourguiba Monastir, Monastir 5019, Tunisia

Laboratory of Biochemistry- Toxicology, University Hospital Fattouma Bourguiba Monastir, Monastir 5019,

Laboratory of Biochemistry, Faculty of Medicine of Monastir, University Hospital Fattouma Bourguiba Monastir, Monastir 5019,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Human plasma paraoxonase1 (PON1) is an esterase catalyzing the hydrolysis of organophosphorus pesticides and other xenobiotics. The aims of this...

Development of an automated method for the determination of human paraoxonase1 activity

Article Category: Original article

Data publikacji: 04 lut 2017

Zakres stron: 217 - 224

DOI: https://doi.org/10.5372/1905-7415.0502.027

Słowa kluczowe
Automated method, paraoxonase1 activity, organophosphate pesticides and nerve agents intoxication

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Development of an automated method for the determination of human paraoxonase1 activity

Article Category: Original article

Data publikacji: 04 lut 2017

Zakres stron: 217 - 224

DOI: https://doi.org/10.5372/1905-7415.0502.027

Słowa kluczoweAutomated method, paraoxonase1 activity, organophosphate pesticides and nerve agents intoxication

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Słowa kluczowe
Automated method, paraoxonase1 activity, organophosphate pesticides and nerve agents intoxication