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Reduced Endothelial Progenitor Cells: A Possible Biomarker for Idiopathic Fetal Growth Restriction in Human Pregnancies

Apurva Singh
Apurva Singh
, 
Shyam Pyari Jaiswar
Shyam Pyari Jaiswar
, 
Apala Priyadarshini
Apala Priyadarshini
 oraz 
Sujata Deo
Sujata Deo
   | 22 lis 2023
Journal of Mother and Child's Cover Image
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Article Category: Original article
Data publikacji: 22 lis 2023
Zakres stron: 182 - 189
Otrzymano: 17 sty 2023
Przyjęty: 06 paź 2023
DOI: https://doi.org/10.34763/jmotherandchild.20232701.d-23-00014
Słowa kluczowe
© 2023 Apurva Singh et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1.

Typical flow cytometry gating technique for circulating Endothelial Progenitor Cells (EPCs) detection. (A) To filter out granulocytes, dead cells, and debris, mononuclear cells were gated in a forward-scatter (FSC) side-scatter (SSC) plot by means of blood samples. Then, cells were successively gated depending on the expression of (B) CD45dim and CD34, (C) Kinase domain receptor (KDR), and (D) CD133. FMO (fluorescence minus one) staining was used to create the gates depicting the (E) KDR and (F) CD133 events. EPCs in circulation were classified as CD45dim/CD34/KDR or CD45dim/CD34/KDR/CD133 cells.
Typical flow cytometry gating technique for circulating Endothelial Progenitor Cells (EPCs) detection. (A) To filter out granulocytes, dead cells, and debris, mononuclear cells were gated in a forward-scatter (FSC) side-scatter (SSC) plot by means of blood samples. Then, cells were successively gated depending on the expression of (B) CD45dim and CD34, (C) Kinase domain receptor (KDR), and (D) CD133. FMO (fluorescence minus one) staining was used to create the gates depicting the (E) KDR and (F) CD133 events. EPCs in circulation were classified as CD45dim/CD34/KDR or CD45dim/CD34/KDR/CD133 cells.

Figure 2.

Expression of circulating Endothelial Progenitor Cells (EPCs) during healthy and Intra-uterine Growth Restriction (FGR) complicated pregnancy in maternal blood. (A) Pregnancies complicated by FGR at comparable gestational ages resulted in considerably decreased levels of EPCs, which were recognized by flow cytometry as CD45dim/CD34/KDR cells, in the I trimester when differentiated with non-pregnant women and continued to decrease. (B) There were no appreciable differences in EPCs classified as CD45dim/CD34/KDR/CD133 cells between FGR and healthy pregnancies. *p < 0.05, **p < 0.02, ***p ≤ 0.001.
Expression of circulating Endothelial Progenitor Cells (EPCs) during healthy and Intra-uterine Growth Restriction (FGR) complicated pregnancy in maternal blood. (A) Pregnancies complicated by FGR at comparable gestational ages resulted in considerably decreased levels of EPCs, which were recognized by flow cytometry as CD45dim/CD34/KDR cells, in the I trimester when differentiated with non-pregnant women and continued to decrease. (B) There were no appreciable differences in EPCs classified as CD45dim/CD34/KDR/CD133 cells between FGR and healthy pregnancies. *p < 0.05, **p < 0.02, ***p ≤ 0.001.

Figure 3.

Concentration of Placental Growth Factor (PlGF) and Stromal Derived Factor (SDF-1) in healthy and Fetal Growth Restriction (FGR) pregnancy. (A) During healthy pregnancy, PlGF levels gradually increased; however, at the same gestational age, they were considerably lower in FGR than in healthy pregnancy. (B) SDF-1 levels grew during normal pregnancy, peaking in the first trimester and gradually decreasing as the pregnancy progressed, although they were lower in FGR than during normal pregnancy. *p < 0.05, **p < 0.02, ***p ≤ 0.001.
Concentration of Placental Growth Factor (PlGF) and Stromal Derived Factor (SDF-1) in healthy and Fetal Growth Restriction (FGR) pregnancy. (A) During healthy pregnancy, PlGF levels gradually increased; however, at the same gestational age, they were considerably lower in FGR than in healthy pregnancy. (B) SDF-1 levels grew during normal pregnancy, peaking in the first trimester and gradually decreasing as the pregnancy progressed, although they were lower in FGR than during normal pregnancy. *p < 0.05, **p < 0.02, ***p ≤ 0.001.
eISSN:
2719-535X
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
Medicine, Clinical Medicine, Pediatrics and Juvenile Medicine, Paediatric Haematology and Oncology, Public Health
Kanał RSS czasopisma