Otwarty dostęp

Point-of-Care Verification of Blood Culture Volume in Neonates: A Feasibility Trial

Justyna Romańska
Justyna Romańska
, 
Tomasz Wawrzoniak
Tomasz Wawrzoniak
, 
Dominika Hołowaty
Dominika Hołowaty
, 
Natalia Mazanowska
Natalia Mazanowska
 oraz 
Paweł Krajewski
Paweł Krajewski
   | 10 sie 2023
Journal of Mother and Child's Cover Image
O artykule
Poprzedni artykuł
Następny artykuł
Zacytuj
Article Category: Research Article
Data publikacji: 10 sie 2023
Zakres stron: 83 - 92
Otrzymano: 24 paź 2022
Przyjęty: 01 lut 2023
DOI: https://doi.org/10.34763/jmotherandchild.20232701.d-22-00063
Słowa kluczowe
© 2023 Justyna Romańska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1.

Percentage of blood culture submissions with calculated volume by month. The horizontal line represents the pre-established criterion of 70% indicating the feasibility of the intervention.
Percentage of blood culture submissions with calculated volume by month. The horizontal line represents the pre-established criterion of 70% indicating the feasibility of the intervention.

Figure 2.

Blood volume summary by source of the material. The horizontal line depicts the minimum blood volume recommended for culture.
Blood volume summary by source of the material. The horizontal line depicts the minimum blood volume recommended for culture.

Figure S1.

Flow chart of included blood culture submissions throughout the study period.
Flow chart of included blood culture submissions throughout the study period.

Volumes submitted for culture according to source of the material

Blood volume measurement [ml] Material source p
Cord blood Umbilical catheter Peripheral vessel
Mean ± SD 1.72 ± 0.78a 1.20 ± 0.28a, b 1.45 ± 0.40b 0.006
Range 0.42 – 4.05 0.71 – 1.85 0.71 – 2.90

Proportion of positivity with adequate and inadequate blood culture volume

Positive blood cultures Adequate volume cultures ≥ 1 ml Inadequate volume cultures < 1 ml p
True-positive 13/179 = 7.26% 2/26 = 7.69% > 0.999
False-positive 8/179 = 4.47% 1/26 = 3.85% > 0.999

Volumes submitted for culture according to birthweight category

Blood volume measurement [ml] Birthweight All birthweight category samples p
≤ 1500 g 1501–2500 g > 2500 g
Mean ± SD 1.38 ± 0.42a 1.49 ± 0.56 1.68 ± 0.70a 1.52 ± 0.58 0.004
Range 0.42 – 2.68 0.68 – 3.53 0.69 – 4.05 0.42 – 4.05

Blood culture submissions characteristics

n (%)
Samples weighed 205 (100.0)
Samples with volume estimation 196 (95.6)
Birthweight 205
  ≤ 1500 g 53 (25.9)
  1501–2500 g 90 (43.9)
  > 2500 g 62 (30.2)
Material source 205
  Cord blood 72 (35.1)
  Umbilical catheter 20 (9.8)
  Peripheral vessel 112 (54.6)
  Not specified 1 (0.5)
Positive blood cultures 24
  True-positive blood cultures 15 (62.5)
  False-positive blood cultures 9 (37.5)

Comparison of blood volume measurement versus estimation

Blood volume measurement [ml]
< 1 ml ≥ 1 ml Total
Blood volume estimation [ml] < 1 ml 4 (2.0) 1 (0.5) 5 (2.6)
≥ 1 ml 22 (11.2) 169 (86.2) 191 (97.4)
Total 26 (13.3) 170 (86.7) 196 (100.0)

Medical personnel opinion on difficulty of blood culture collection and necessity of blood sample measurement.

Total group Doctors Nurses p
n 36 14 22
Blood culture collection is:
  Easy 11 (30.6) 6 (42.9) 5 (22.7) 0.469
  Moderately difficult 21 (58.3) 7 (50.0) 14 (63.6)
  Difficult 4 (11.1) 1 (7.1) 3 (13.6)
  Don’t know - - -
Measurement of blood volume collected for culture is:
  Unnecessary 2 (5.6) - 2 (9.1) 0.244
  Necessary, but a substantial impediment 3 (8.3) 1 (7.1) 2 (9.1)
  Necessary and not a substantial impediment 27 (75.0) 13 (92.9) 14 (63.6)
  Don’t know 4 (11.1) - 4 (18.2)
eISSN:
2719-535X
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
Medicine, Clinical Medicine, Pediatrics and Juvenile Medicine, Paediatric Haematology and Oncology, Public Health
Kanał RSS czasopisma