Screening of Toxin Genes in Methicillin-Resistant Staphylococcus aureus Clinical Isolates from a Hospital Setting in a Tertiary Hospital in Northern Cyprus

Abbasi Montazeri E, Khosravi AD, Khazaei S, Sabbagh A. Prevalence of methicillin resistance and superantigenic toxins in Staphylococcus aureus strains isolated from patients with cancer. BMC Microbiol. 2021 Dec;21(1):262. https://doi.org/10.1186/s12866-021-02319-7 Abbasi Montazeri E Khosravi AD Khazaei S Sabbagh A Prevalence of methicillin resistance and superantigenic toxins in Staphylococcus aureus strains isolated from patients with cancer BMC Microbiol 2021 Dec211262 https://doi.org/10.1186/s12866-021-02319-710.1186/s12866-021-02319-7848260134587897Search in Google Scholar

Algammal AM, Hetta HF, Elkelish A, Alkhalifah DHH, Hozzein WN, Batiha GES, El Nahhas N, Mabrok MA. Methicillin-resistant Staphylococcus aureus (MRSA): One health perspective approach to the bacterium epidemiology, virulence factors, antibiotic-resistance, and zoonotic impact. Infect Drug Resist. 2020 Sep; 13:3255–3265. https://doi.org/10.2147/IDR.S272733 Algammal AM Hetta HF Elkelish A Alkhalifah DHH Hozzein WN Batiha GES El Nahhas N Mabrok MA Methicillin-resistant Staphylococcus aureus (MRSA): One health perspective approach to the bacterium epidemiology, virulence factors, antibiotic-resistance, and zoonotic impact Infect Drug Resist 2020 Sep 133255 3265 https://doi.org/10.2147/IDR.S27273310.2147/IDR.S272733751982933061472Search in Google Scholar

Amin DHM, Guler E, Baddal B. Prevalence of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus clinical isolates at a university hospital in Northern Cyprus: A pilot study. BMC Res Notes. 2020 Oct 20;13(1):490. https://doi.org/10.1186/s13104-020-05339-0 Amin DHM Guler E Baddal B Prevalence of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus clinical isolates at a university hospital in Northern Cyprus: A pilot study BMC Res Notes 2020 Oct 20131490 https://doi.org/10.1186/s13104-020-05339-010.1186/s13104-020-05339-0757672133081819Search in Google Scholar

Barbosa C, Nogueira S, Gadanho M, Chaves S. Chapter 7 – DNA extraction: finding the most suitable method. In: Cook N, D’Agostino M, Thompson KC, editors. Molecular microbial diagnostic methods. San Diego (USA): Academic Press; 2016. p. 135–154. https://doi.org/10.1016/B978-0-12-416999-9.00007-1 Barbosa C Nogueira S Gadanho M Chaves S Chapter 7 – DNA extraction: finding the most suitable method. In: Cook N, D’Agostino M, Thompson KC, editors. Molecular microbial diagnostic methods San Diego (USA) Academic Press; 2016 p 135 154 https://doi.org/10.1016/B978-0-12-416999-9.00007-110.1016/B978-0-12-416999-9.00007-1Search in Google Scholar

Barcudi D, Sosa EJ, Lamberghini R, Garnero A, Tosoroni D, Decca L, Gonzalez L, Kuyuk MA, Lopez T, Herrero I, et al.; Study Group of S. aureus in Córdoba, Argentina. MRSA dynamic circulation between the community and the hospital setting: new insights from a cohort study. J Infect. 2020 Jan;80(1):24–37. https://doi.org/10.1016/j.jinf.2019.10.001 Barcudi D Sosa EJ Lamberghini R Garnero A Tosoroni D Decca L Gonzalez L Kuyuk MA Lopez T Herrero I et al Study Group of S. aureus in Córdoba, Argentina. MRSA dynamic circulation between the community and the hospital setting: new insights from a cohort study J Infect 2020 Jan80124 37 https://doi.org/10.1016/j.jinf.2019.10.00110.1016/j.jinf.2019.10.00131606351Search in Google Scholar

Berger S, Kunerl A, Wasmuth S, Tierno P, Wagner K, Brügger J. Menstrual toxic shock syndrome: Case report and systematic review of the literature. Lancet Infect Dis. 2019 Sep;19(9):e313–e321. https://doi.org/10.1016/S1473-3099(19)30041-6 Berger S Kunerl A Wasmuth S Tierno P Wagner K Brügger J Menstrual toxic shock syndrome: Case report and systematic review of the literature Lancet Infect Dis 2019 Sep199e313 e321 https://doi.org/10.1016/S1473-3099(19)30041-610.1016/S1473-3099(19)30041-631151811Search in Google Scholar

Bispo PJM, Ung L, Chodosh J, Gilmore MS. Hospital-associated multidrug-resistant MRSA lineages are trophic to the ocular surface and cause severe microbial keratitis. Front Public Health. 2020 Jun 3;8:204. https://doi.org/10.3389/fpubh.2020.00204 Bispo PJM Ung L Chodosh J Gilmore MS Hospital-associated multidrug-resistant MRSA lineages are trophic to the ocular surface and cause severe microbial keratitis Front Public Health 2020 Jun 38204 https://doi.org/10.3389/fpubh.2020.0020410.3389/fpubh.2020.00204728349432582610Search in Google Scholar

Chang RYK, Nang SC, Chan HK, Li J. Novel antimicrobial agents for combating antibiotic-resistant bacteria. Adv Drug Deliv Rev. 2022 Aug;187:114378. https://doi.org/10.1016/j.addr.2022.114378 Chang RYK Nang SC Chan HK Li J Novel antimicrobial agents for combating antibiotic-resistant bacteria Adv Drug Deliv Rev 2022 Aug187114378 https://doi.org/10.1016/j.addr.2022.11437810.1016/j.addr.2022.11437835671882Search in Google Scholar

Choi JH, Lee H, Choi EH. Antimicrobial resistance and molecular analysis of Staphylococcus aureus in staphylococcal scalded skin syndrome among children in Korea. J Korean Med Sci. 2021;36(3):e22. https://doi.org/10.3346/jkms.2021.36.e22 Choi JH Lee H Choi EH Antimicrobial resistance and molecular analysis of Staphylococcus aureus in staphylococcal scalded skin syndrome among children in Korea J Korean Med Sci 2021363e22 https://doi.org/10.3346/jkms.2021.36.e2210.3346/jkms.2021.36.e22781358633463096Search in Google Scholar

EUCAST. Antimicrobial susceptibility testing EUCAST disk diffusion method. Version 8.0 January 2020. Basel (Switzerland): The European Committee on Antimicrobial Susceptibility Testing; 2020 [cited 2022 Apr 8]. Available from https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Disk_test_documents/2020_manuals/Manual_v_8.0_EUCAST_Disk_Test_2020.pdf EUCAST. Antimicrobial susceptibility testing EUCAST disk diffusion method. Version 8.0 January 2020 Basel (Switzerland) The European Committee on Antimicrobial Susceptibility Testing 2020 [cited 2022 Apr 8]. Available from https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Disk_test_documents/2020_manuals/Manual_v_8.0_EUCAST_Disk_Test_2020.pdfSearch in Google Scholar

Gagliotti C, Högberg LD, Billström H, Eckmanns T, Giske CG, Heuer OE, Jarlier V, Kahlmeter G, Lo Fo Wong D, Monen J, et al.; EARS-Net study group participants. Staphylococcus aureus bloodstream infections: diverging trends of meticillin-resistant and meticillin-susceptible isolates, EU/EEA, 2005 to 2018. Euro Surveill. 2021 Nov 18;26(46):2002094. https://doi.org/10.2807/1560-7917.ES.2021.26.46.2002094 Gagliotti C Högberg LD Billström H Eckmanns T Giske CG Heuer OE Jarlier V Kahlmeter G Lo Fo Wong D Monen J et al EARS-Net study group participants. Staphylococcus aureus bloodstream infections: diverging trends of meticillin-resistant and meticillin-susceptible isolates, EU/EEA 2005 to 2018. Euro Surveill. 2021 Nov 1826462002094 https://doi.org/10.2807/1560-7917.ES.2021.26.46.200209410.2807/1560-7917.ES.2021.26.46.2002094860340634794536Search in Google Scholar

He S, Deng Q, Liang B, Yu F, Yu X, Guo D, Liu X, Dong H. Suppressing alpha-hemolysin as potential target to screen of flavonoids to combat bacterial coinfection. Molecules. 2021 Dec 14;26(24):7577. https://doi.org/10.3390/molecules26247577 He S Deng Q Liang B Yu F Yu X Guo D Liu X Dong H Suppressing alpha-hemolysin as potential target to screen of flavonoids to combat bacterial coinfection Molecules 2021 Dec 1426247577 https://doi.org/10.3390/molecules2624757710.3390/molecules26247577870938534946657Search in Google Scholar

Horino T, Hori S. Metastatic infection during Staphylococcus aureus bacteremia. J Infect Chemother. 2020 Feb;26(2):162–169. https://doi.org/10.1016/j.jiac.2019.10.003 Horino T Hori S Metastatic infection during Staphylococcus aureus bacteremia J Infect Chemother 2020 Feb262162 169 https://doi.org/10.1016/j.jiac.2019.10.00310.1016/j.jiac.2019.10.00331676266Search in Google Scholar

Jernigan JA, Hatfield KM, Wolford H, Nelson RE, Olubajo B, Reddy SC, McCarthy N, Paul P, McDonald LC, Kallen A, et al. Multidrug- resistant bacterial infections in U.S. hospitalized patients, 2012–2017. N Engl J Med. 2020 Apr 02;382(14):1309–1319. https://doi.org/10.1056/NEJMoa1914433 Jernigan JA Hatfield KM Wolford H Nelson RE Olubajo B Reddy SC McCarthy N Paul P McDonald LC Kallen A et al Multidrug- resistant bacterial infections in U.S hospitalized patients, 2012–2017. N Engl J Med 2020 Apr 02382141309 1319 https://doi.org/10.1056/NEJMoa191443310.1056/NEJMoa191443332242356Search in Google Scholar

Jorgensen SCJ, Lagnf AM, Bhatia S, Singh NB, Shammout LK, Davis SL, Rybak MJ. Diagnostic stewardship: A clinical decision rule for blood cultures in community-onset methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infections. Infect Dis Ther. 2019 Jun;8(2):229–242. https://doi.org/10.1007/s40121-019-0238-1 Jorgensen SCJ Lagnf AM Bhatia S Singh NB Shammout LK Davis SL Rybak MJ Diagnostic stewardship: A clinical decision rule for blood cultures in community-onset methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infections Infect Dis Ther 2019 Jun82229 242 https://doi.org/10.1007/s40121-019-0238-110.1007/s40121-019-0238-1652257730783995Search in Google Scholar

Junnila J, Hirvioja T, Rintala E, Auranen K, Rantakokko-Jalava K, Silvola J, Lindholm L, Gröndahl-Yli-Hannuksela K, Marttila H, Vuopio J. Changing epidemiology of methicillin-resistant Staphylococcus aureus in a low endemicity area – new challenges for MRSA control. Eur J Clin Microbiol Infect Dis. 2020 Dec;39(12):2299–2307. https://doi.org/10.1007/s10096-020-03824-9 Junnila J Hirvioja T Rintala E Auranen K Rantakokko-Jalava K Silvola J Lindholm L Gröndahl-Yli-Hannuksela K Marttila H Vuopio J Changing epidemiology of methicillin-resistant Staphylococcus aureus in a low endemicity area – new challenges for MRSA control Eur J Clin Microbiol Infect Dis 2020 Dec39122299 2307 https://doi.org/10.1007/s10096-020-03824-910.1007/s10096-020-03824-9766980031989375Search in Google Scholar

Kot B, Wierzchowska K, Piechota M, Gruzewska A. Antimicrobial resistance patterns in methicillin-resistant Staphylococcus aureus from patients hospitalized during 2015-2017 in hospitals in Poland. Med Princ Pract. 2020;29(1):61–68. https://doi.org/10.1159/000501788 Kot B Wierzchowska K Piechota M Gruzewska A Antimicrobial resistance patterns in methicillin-resistant Staphylococcus aureus from patients hospitalized during 2015-2017 in hospitals in Poland Med Princ Pract 202029161 68 https://doi.org/10.1159/00050178810.1159/000501788702485831256152Search in Google Scholar

Kourtis AP, Hatfield K, Baggs J, Mu Y, See I, Epson E, Nadle J, Kainer MA, Dumyati G, Petit S, et al.; Emerging Infections Program MRSA author group. Vital Signs: Epidemiology and recent trends in methicillin-resistant and in methicillin- susceptible Staphylococcus aureus bloodstream infections – United States. MMWR Morb Mortal Wkly Rep. 2019 Mar 08;68(9):214–219. https://doi.org/10.15585/mmwr.mm6809e1 Kourtis AP Hatfield K Baggs J Mu Y See I Epson E Nadle J Kainer MA Dumyati G Petit S et al Emerging Infections Program MRSA author group. Vital Signs: Epidemiology and recent trends in methicillin-resistant and in methicillin- susceptible Staphylococcus aureus bloodstream infections – United States MMWR Morb Mortal Wkly Rep 2019 Mar 08689214 219 https://doi.org/10.15585/mmwr.mm6809e110.15585/mmwr.mm6809e1642196730845118Search in Google Scholar

Lebughe M, Phaku P, Niemann S, Mumba D, Peters G, Muyembe-Tamfum JJ, Mellmann A, Strauß L, Schaumburg F. The impact of the Staphylococcus aureus virulome on infection in a developing country: A cohort study. Front Microbiol. 2017 Aug 29;8(AUG):1662. https://doi.org/10.3389/fmicb.2017.01662 Lebughe M Phaku P Niemann S Mumba D Peters G Muyembe-Tamfum JJ Mellmann A Strauß L Schaumburg F The impact of the Staphylococcus aureus virulome on infection in a developing country: A cohort study Front Microbiol 2017 Aug 298AUG1662 https://doi.org/10.3389/fmicb.2017.0166210.3389/fmicb.2017.01662558193428900424Search in Google Scholar

Li X, Huang T, Xu K, Li C, Li Y. Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China. BMC Infect Dis. 2019 Dec;19(1):873. https://doi.org/10.1186/s12879-019-4547-5 Li X Huang T Xu K Li C Li Y Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China BMC Infect Dis 2019 Dec191873 https://doi.org/10.1186/s12879-019-4547-510.1186/s12879-019-4547-5680558231640587Search in Google Scholar

Li Z, Zhuang H, Wang G, Wang H, Dong Y. Prevalence, predictors, and mortality of bloodstream infections due to methicillin-resistant Staphylococcus aureus in patients with malignancy: Systemic review and meta-analysis. BMC Infect Dis. 2021 Dec;21(1):74. https://doi.org/10.1186/s12879-021-05763-y Li Z Zhuang H Wang G Wang H Dong Y Prevalence, predictors, and mortality of bloodstream infections due to methicillin-resistant Staphylococcus aureus in patients with malignancy: Systemic review and meta-analysis BMC Infect Dis 2021 Dec21174 https://doi.org/10.1186/s12879-021-05763-y10.1186/s12879-021-05763-y780979833446122Search in Google Scholar

Liu B, Sun H, Pan Y, Zhai Y, Cai T, Yuan X, Gao Y, He D, Liu J, Yuan L, et al. Prevalence, resistance pattern, and molecular characterization of Staphylococcus aureus isolates from healthy animals and sick populations in Henan Province, China. Gut Pathog. 2018 Jul 17;10(1):31. https://doi.org/10.1186/s13099-018-0254-9 Liu B Sun H Pan Y Zhai Y Cai T Yuan X Gao Y He D Liu J Yuan L et al Prevalence, resistance pattern, and molecular characterization of Staphylococcus aureus isolates from healthy animals and sick populations in Henan Province, China Gut Pathog 2018 Jul 1710131 https://doi.org/10.1186/s13099-018-0254-910.1186/s13099-018-0254-9604877430026814Search in Google Scholar

Lu H, Zhao L, Si Y, Jian Y, Wang Y, Li T, Dai Y, Huang Q, Ma X, He L, et al. The surge of hypervirulent ST398 MRSA lineage with >higher biofilm-forming ability is a critical threat to clinics. Front Microbiol. 2021 Mar 4;12(March):636788. https://doi.org/10.3389/fmicb.2021.636788 Lu H Zhao L Si Y Jian Y Wang Y Li T Dai Y Huang Q Ma X He L et al The surge of hypervirulent ST398 MRSA lineage with >higher biofilm-forming ability is a critical threat to clinics Front Microbiol 2021 Mar 412March636788 https://doi.org/10.3389/fmicb.2021.63678810.3389/fmicb.2021.636788796981533746929Search in Google Scholar

Maalej SM, Trabelsi JJ, Claude-alexandre G, Boutiba I, Mastouri M, Besbes S, Barguellil F, Laurent F, Hammami A. Antimicrobial susceptibility and molecular epidemiology of methicillin-resistant Staphylococcus aureus in Tunisia: Results of a multicenter study. J Infect Dis Epidemiol. 2019 Mar 11;5(2):071. https://doi.org/10.23937/2474-3658/1510071 Maalej SM Trabelsi JJ Claude-alexandre G Boutiba I Mastouri M Besbes S Barguellil F Laurent F Hammami A Antimicrobial susceptibility and molecular epidemiology of methicillin-resistant Staphylococcus aureus in Tunisia: Results of a multicenter study J Infect Dis Epidemiol 2019 Mar 1152071 https://doi.org/10.23937/2474-3658/151007110.23937/2474-3658/1510071Search in Google Scholar

Mariutti RB, Tartaglia NR, Seyffert N, de Paula Castro T, Arni RK, Azevedo VA, Le Loir Y, Nishifuji K. Exfoliative toxins of Staphylococcus aureus. In: Enany S, Alexander LEC, editors. The rise of virulence and antibiotic resistance in Staphylococcus aureus. London (UK): IntechOpen; 2017. https://doi.org/10.5772/66528 Mariutti RB Tartaglia NR Seyffert N de Paula Castro T Arni RK Azevedo VA Le Loir Y Nishifuji K Exfoliative toxins of Staphylococcus aureus In Enany S Alexander LEC editors The rise of virulence and antibiotic resistance in Staphylococcus aureus London (UK) IntechOpen 2017 https://doi.org/10.5772/6652810.5772/66528Search in Google Scholar

Mohseni M, Rafiei F, Ghaemi EA. High frequency of exfoliative toxin genes among Staphylococcus aureus isolated from clinical specimens in the north of Iran: Alarm for the health of individuals under risk. Iran J Microbiol. 2018 Jun; 10(3):158–165. Mohseni M Rafiei F Ghaemi EA High frequency of exfoliative toxin genes among Staphylococcus aureus isolated from clinical specimens in the north of Iran: Alarm for the health of individuals under risk Iran J Microbiol 2018 Jun 103158 165Search in Google Scholar

Naorem RS, Pangabam BD, Bora SS, Goswami G, Barooah M, Hazarika DJ, Fekete C. Identification of putative vaccine and drug targets against the methicillin-resistant Staphylococcus aureus by reverse vaccinology and subtractive genomics approaches. Molecules. 2022 Mar 24;27(7):2083. https://doi.org/10.3390/molecules27072083 Naorem RS Pangabam BD Bora SS Goswami G Barooah M Hazarika DJ Fekete C Identification of putative vaccine and drug targets against the methicillin-resistant Staphylococcus aureus by reverse vaccinology and subtractive genomics approaches Molecules 2022 Mar 242772083 https://doi.org/10.3390/molecules2707208310.3390/molecules27072083900051135408485Search in Google Scholar

Nisar S, Kirkpatrick LD, Shupp JW. Bacterial virulence factors and their contribution to pathophysiology after thermal injury. Surg Infect (Larchmt). 2021 Feb;22(1):69–76. https://doi.org/10.1089/sur.2020.188 Nisar S Kirkpatrick LD Shupp JW Bacterial virulence factors and their contribution to pathophysiology after thermal injury Surg Infect (Larchmt) 2021 Feb22169 76 https://doi.org/10.1089/sur.2020.18810.1089/sur.2020.18832735479Search in Google Scholar

Okwu MU, Olley M, Akpoka AO, Izevbuwa OE. Methicillin-resistant Staphylococcus aureus (MRSA) and anti-MRSA activities of extracts of some medicinal plants: A brief review. AIMS Microbiol. 2019 Apr 15;5(2):117–137. https://doi.org/10.3934/microbiol.2019.2.117 Okwu MU Olley M Akpoka AO Izevbuwa OE Methicillin-resistant Staphylococcus aureus (MRSA) and anti-MRSA activities of extracts of some medicinal plants: A brief review AIMS Microbiol 2019 Apr 1552117 137 https://doi.org/10.3934/microbiol.2019.2.11710.3934/microbiol.2019.2.117664290731384707Search in Google Scholar

Pannewick B, Baier C, Schwab F, Vonberg RP. Infection control measures in nosocomial MRSA outbreaks – Results of a systematic analysis. PLoS One. 2021 Apr 7;16(4):e0249837. https://doi.org/10.1371/journal.pone.0249837 Pannewick B Baier C Schwab F Vonberg RP Infection control measures in nosocomial MRSA outbreaks – Results of a systematic analysis PLoS One 2021 Apr 7164e0249837 https://doi.org/10.1371/journal.pone.024983710.1371/journal.pone.0249837802605633826678Search in Google Scholar

Papadimitriou-Olivgeris M, Drougka E, Fligou F, Dodou V, Kolonitsiou F, Filos KS, Anastassiou ED, Petinaki E, Marangos M, Spiliopoulou I. Spread of Tst-positive Staphylococcus aureus strains belonging to ST30 clone among patients and healthcare workers in two intensive care units. Toxins (Basel). 2017 Sep 4;9(9):270. https://doi.org/10.3390/TOXINS9090270 Papadimitriou-Olivgeris M Drougka E Fligou F Dodou V Kolonitsiou F Filos KS Anastassiou ED Petinaki E Marangos M Spiliopoulou I Spread of Tst-positive Staphylococcus aureus strains belonging to ST30 clone among patients and healthcare workers in two intensive care units Toxins (Basel) 2017 Sep 499270 https://doi.org/10.3390/TOXINS909027010.3390/toxins9090270561820328869541Search in Google Scholar

Park KH, Greenwood-Quaintance KE, Cunningham SA, Raja-gopalan G, Chia N, Jeraldo PR, Mandrekar J, Patel R. Lack of correlation of virulence gene profiles of Staphylococcus aureus bacteremia isolates with mortality. Microb Pathog. 2019 Aug;133:103543. https://doi.org/10.1016/j.micpath.2019.103543 Park KH Greenwood-Quaintance KE Cunningham SA Raja-gopalan G Chia N Jeraldo PR Mandrekar J Patel R Lack of correlation of virulence gene profiles of Staphylococcus aureus bacteremia isolates with mortality Microb Pathog 2019 Aug133103543 https://doi.org/10.1016/j.micpath.2019.10354310.1016/j.micpath.2019.10354331102653Search in Google Scholar

Peterson JC, Durkee H, Miller D, Maestre-Mesa J, Arboleda A, Aguilar MC, Relhan N, Flynn HW Jr, Amescua G, Parel JM, et al. Molecular epidemiology and resistance profiles among healthcare-and community-associated Staphylococcus aureus keratitis isolates. Infect Drug Resist. 2019 Apr;12:831–843. https://doi.org/10.2147/IDR.S190245 Peterson JC Durkee H Miller D Maestre-Mesa J Arboleda A Aguilar MC Relhan N Flynn HW Jr Amescua G Parel JM et al Molecular epidemiology and resistance profiles among healthcare-and community-associated Staphylococcus aureus keratitis isolates Infect Drug Resist 2019 Apr12831 843 https://doi.org/10.2147/IDR.S19024510.2147/IDR.S190245646946931043797Search in Google Scholar

Pomorska-Wesołowska M, Różańska A, Natkaniec J, Gryglewska B, Szczypta A, Dzikowska M, Chmielarczyk A, Wójkowska-Mach J. Longevity and gender as the risk factors of methicillin-resistant Staphylococcus aureus infections in southern Poland. BMC Geriatr. 2017 Dec;17(1):51. https://doi.org/10.1186/s12877-017-0442-3 Pomorska-Wesołowska M Różańska A Natkaniec J Gryglewska B Szczypta A Dzikowska M Chmielarczyk A Wójkowska-Mach J Longevity and gender as the risk factors of methicillin-resistant Staphylococcus aureus infections in southern Poland BMC Geriatr 2017 Dec17151 https://doi.org/10.1186/s12877-017-0442-310.1186/s12877-017-0442-3530324328187785Search in Google Scholar

Rahman MM, Amin KB, Rahman SMM, Khair A, Rahman M, Hossain A, Rahman AKMA, Parvez MS, Miura N, Alam MM. Investigation of methicillin-resistant Staphylococcus aureus among clinical isolates from humans and animals by culture methods and multiplex PCR. BMC Vet Res. 2018 Oct 3;14(1):300. https://doi.org/10.1186/s12917-018-1611-0 Rahman MM Amin KB Rahman SMM Khair A Rahman M Hossain A Rahman AKMA Parvez MS Miura N Alam MM Investigation of methicillin-resistant Staphylococcus aureus among clinical isolates from humans and animals by culture methods and multiplex PCR BMC Vet Res 2018 Oct 3141300 https://doi.org/10.1186/s12917-018-1611-010.1186/s12917-018-1611-0616906430285752Search in Google Scholar

Rasheed NA, Hussein NR. Characterization of different virulent factors in methicillin-resistant Staphylococcus aureus isolates recovered from Iraqis and Syrian refugees in Duhok city, Iraq. PLoS One. 2020 Aug 17;15(8):e0237714. https://doi.org/10.1371/journal.pone.0237714 Rasheed NA Hussein NR Characterization of different virulent factors in methicillin-resistant Staphylococcus aureus isolates recovered from Iraqis and Syrian refugees in Duhok city, Iraq PLoS One 2020 Aug 17158e0237714 https://doi.org/10.1371/journal.pone.023771410.1371/journal.pone.0237714743075332804961Search in Google Scholar

Schulz M, Calabrese S, Hausladen F, Wurm H, Drossart D, Stock K, Sobieraj AM, Eichenseher F, Loessner MJ, Schmelcher M, et al. Point-of-care testing system for digital single cell detection of MRSA directly from nasal swabs. Lab Chip. 2020 Jul 14;20(14):2549–2561. https://doi.org/10.1039/D0LC00294A Schulz M Calabrese S Hausladen F Wurm H Drossart D Stock K Sobieraj AM Eichenseher F Loessner MJ Schmelcher M et al Point-of-care testing system for digital single cell detection of MRSA directly from nasal swabs Lab Chip 2020 Jul 1420142549 2561 https://doi.org/10.1039/D0LC00294A10.1039/D0LC00294A32568322Search in Google Scholar

Shahini Shams-Abadi M, Halaji M, Hoseini-Alfatemi SM, Gholipour A, Mojtahedi A, Sedigh Ebrahim-Saraie H. Epidemiology of toxic shock syndrome toxin-1 harboring Staphylococcus aureus obtained from clinical samples in Iran: A systematic review and meta-analysis. Ann Ig. 2018 Sep–Oct;30(5):391–400. https://doi.org/10.7416/ai.2018.2239 Shahini Shams-Abadi M Halaji M Hoseini-Alfatemi SM Gholipour A Mojtahedi A Sedigh Ebrahim-Saraie H Epidemiology of toxic shock syndrome toxin-1 harboring Staphylococcus aureus obtained from clinical samples in Iran: A systematic review and meta-analysis Ann Ig 2018 Sep–Oct305391 400 https://doi.org/10.7416/ai.2018.2239Search in Google Scholar

Shettigar K, Murali TS. Virulence factors and clonal diversity of Staphylococcus aureus in colonization and wound infection with emphasis on diabetic foot infection. Eur J Clin Microbiol Infect Dis. 2020 Dec;39(12):2235–2246. https://doi.org/10.1007/s10096-020-03984-8 Shettigar K Murali TS Virulence factors and clonal diversity of Staphylococcus aureus in colonization and wound infection with emphasis on diabetic foot infection Eur J Clin Microbiol Infect Dis 2020 Dec39122235 2246 https://doi.org/10.1007/s10096-020-03984-810.1007/s10096-020-03984-8766977932683595Search in Google Scholar

Thorlacius-Ussing L, Sandholdt H, Larsen AR, Petersen A, Benfield T. Age-dependent increase in incidence of Staphylococcus aureus bacteremia, Denmark, 2008–2015. Emerg Infect Dis. 2019 May;25(5):875–882. https://doi.org/10.3201/eid2505.181733 Thorlacius-Ussing L Sandholdt H Larsen AR Petersen A Benfield T Age-dependent increase in incidence of Staphylococcus aureus bacteremia, Denmark, 2008–2015 Emerg Infect Dis 2019 May255875 882 https://doi.org/10.3201/eid2505.18173310.3201/eid2505.181733647819631002300Search in Google Scholar

Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG Jr. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev. 2015 Jul;28(3):603–661. https://doi.org/10.1128/CMR.00134-14 Tong SYC Davis JS Eichenberger E Holland TL Fowler VG Jr Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management Clin Microbiol Rev 2015 Jul283603 661 https://doi.org/10.1128/CMR.00134-1410.1128/CMR.00134-14445139526016486Search in Google Scholar

Tsuzuki S, Matsunaga N, Yahara K, Shibayama K, Sugai M, Ohmagari N. Disease burden of bloodstream infections caused by antimicrobial-resistant bacteria: A population-level study, Japan, 2015–2018. Int J Infect Dis. 2021 Jul;108:119–124. https://doi.org/10.1016/j.ijid.2021.05.018 Tsuzuki S Matsunaga N Yahara K Shibayama K Sugai M Ohmagari N Disease burden of bloodstream infections caused by antimicrobial-resistant bacteria: A population-level study, Japan, 2015–2018 Int J Infect Dis 2021 Jul108119 124 https://doi.org/10.1016/j.ijid.2021.05.01810.1016/j.ijid.2021.05.01833992765Search in Google Scholar

Tuffs SW, Herfst CA, Baroja ML, Podskalniy VA, DeJong EN, Coleman CEM, McCormick JK. Regulation of toxic shock syndrome toxin‐1 by the accessory gene regulator in Staphylococcus aureus is mediated by the repressor of toxins. Mol Microbiol. 2019 Oct;112(4):1163–1177. https://doi.org/10.1111/mmi.14353 Tuffs SW Herfst CA Baroja ML Podskalniy VA DeJong EN Coleman CEM McCormick JK Regulation of toxic shock syndrome toxin‐1 by the accessory gene regulator in Staphylococcus aureus is mediated by the repressor of toxins Mol Microbiol 2019 Oct11241163 1177 https://doi.org/10.1111/mmi.1435310.1111/mmi.1435331321813Search in Google Scholar

Turner NA, Sharma-Kuinkel BK, Maskarinec SA, Eichenberger EM, Shah PP, Carugati M, Holland TL, Fowler VG Jr. Methicillin-resistant Staphylococcus aureus: An overview of basic and clinical research. Nat Rev Microbiol. 2019 Apr;17(4):203–218. https://doi.org/10.1038/s41579-018-0147-4 Turner NA Sharma-Kuinkel BK Maskarinec SA Eichenberger EM Shah PP Carugati M Holland TL Fowler VG Jr Methicillin-resistant Staphylococcus aureus: An overview of basic and clinical research Nat Rev Microbiol 2019 Apr174203 218 https://doi.org/10.1038/s41579-018-0147-410.1038/s41579-018-0147-4693988930737488Search in Google Scholar

Uehara Y. Current status of staphylococcal cassette chromosome mec (SCCmec). Antibiotics (Basel). 2022 Jan 11;11(1):86. https://doi.org/10.3390/antibiotics11010086 Uehara Y Current status of staphylococcal cassette chromosome mec (SCCmec) Antibiotics (Basel) 2022 Jan 1111186 https://doi.org/10.3390/antibiotics1101008610.3390/antibiotics11010086877272635052963Search in Google Scholar

Urushibara N, Aung MS, Kawaguchiya M, Kobayashi N. Novel staphylococcal cassette chromosome mec (SCCmec) type XIV (5A) and a truncated SCCmec element in SCC composite islands carrying speG in ST5 MRSA in Japan. J Antimicrob Chemother. 2020 Jan 1;75(1):46–50. https://doi.org/10.1093/jac/dkz406 Urushibara N Aung MS Kawaguchiya M Kobayashi N Novel staphylococcal cassette chromosome mec (SCCmec) type XIV (5A) and a truncated SCCmec element in SCC composite islands carrying speG in ST5 MRSA in Japan J Antimicrob Chemother 2020 Jan 175146 50 https://doi.org/10.1093/jac/dkz40610.1093/jac/dkz40631617906Search in Google Scholar

Zhao H, Xu S, Yang H, He C, Xu X, Hu F, Shu W, Gong F, Zhang C, Liu Q. Molecular typing and variations in amount of tst gene expression of TSST-1-producing clinical Staphylococcus aureus isolates. Front Microbiol. 2019 Jun 19;10(JUN):1388. https://doi.org/10.3389/fmicb.2019.01388 Zhao H Xu S Yang H He C Xu X Hu F Shu W Gong F Zhang C Liu Q Molecular typing and variations in amount of tst gene expression of TSST-1-producing clinical Staphylococcus aureus isolates Front Microbiol 2019 Jun 1910JUN1388 https://doi.org/10.3389/fmicb.2019.0138810.3389/fmicb.2019.01388659435631275293Search in Google Scholar

Zhou W, Wu R, Duraiswamy S, Wang W, Zhu L, Wang Z. Development of microfluidic cartridge for culture-free detection of Staphylococcus aureus in blood. J Micromech Microeng. 2021 May 01; 31(5):055012. https://doi.org/10.1088/1361-6439/abf32f Zhou W Wu R Duraiswamy S Wang W Zhu L Wang Z Development of microfluidic cartridge for culture-free detection of Staphylococcus aureus in blood J Micromech Microeng 2021 May 01 315055012 https://doi.org/10.1088/1361-6439/abf32f10.1088/1361-6439/abf32fSearch in Google Scholar