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<p>Whole-genome sequencing and genome mining are recently considered an efficient approach to shine more light on the underlying secondary metabolites of <italic>Streptomyces</italic>. The present study unearths the biosynthetic potential of endophytic SX6 as a promising source of biologically active substances and plant-derived compounds for the first time. Out of 38 isolates associated with <italic>Aegiceras corniculatum</italic> (L.) Blanco, <italic>Streptomyces parvulus</italic> SX6 was highly active against <italic>Pseudomonas aeruginosa</italic> ATCC® 9027™ and methicillin-resistant <italic>Staphylococcus epidermidis</italic> (MRSE) ATCC® 35984™. Additionally, <italic>S. parvulus</italic> SX6 culture extract showed strong cytotoxicity against Hep3B, MCF-7, and A549 cell lines at a concentration of 30 μg/ml, but not in non-cancerous HEK-293 cells. The genome contained 7.69 Mb in size with an average G + C content of 72.8% and consisted of 6,779 protein-coding genes. AntiSMASH analysis resulted in the identification of 29 biosynthetic gene clusters (BGCs) for secondary metabolites. Among them, 4 BGCs showed low similarity (28–67% of genes show similarity) to actinomycin, streptovaricin, and polyoxypeptin gene clusters, possibly attributed to antibacterial and anticancer activities observed. In addition, the complete biosynthetic pathways of plant-derived compounds, including daidzein and genistein were identified using genome mining and HPLC-DAD-MS analysis. These findings portray an exciting avenue for future characterization of promising secondary metabolites from mangrove endophytic <italic>S. parvulus</italic>.</p>
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</abstract>

Whole-genome sequencing and genome mining are recently considered an efficient approach to shine more light on the underlying secondary metabolites of Streptomyces. The present study unearths the biosynthetic potential of endophytic SX6 as a promising source of biologically active substances and plant-derived compounds for the first time. Out of 38 isolates associated with Aegiceras corniculatum (L.) Blanco, Streptomyces parvulus SX6 was highly active against Pseudomonas aeruginosa ATCC® 9027™ and methicillin-resistant Staphylococcus epidermidis (MRSE) ATCC® 35984™. Additionally, S. parvulus SX6 culture extract showed strong cytotoxicity against Hep3B, MCF-7, and A549 cell lines at a concentration of 30 μg/ml, but not in non-cancerous HEK-293 cells. The genome contained 7.69 Mb in size with an average G + C content of 72.8% and consisted of 6,779 protein-coding genes. AntiSMASH analysis resulted in the identification of 29 biosynthetic gene clusters (BGCs) for secondary metabolites. Among them, 4 BGCs showed low similarity (28–67% of genes show similarity) to actinomycin, streptovaricin, and polyoxypeptin gene clusters, possibly attributed to antibacterial and anticancer activities observed. In addition, the complete biosynthetic pathways of plant-derived compounds, including daidzein and genistein were identified using genome mining and HPLC-DAD-MS analysis. These findings portray an exciting avenue for future characterization of promising secondary metabolites from mangrove endophytic S. parvulus.

Genome-Guided Investigation Provides New Insights into Secondary Metabolites of <italic>Streptomyces parvulus</italic> SX6 from <italic>Aegiceras corniculatum</italic>

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Genome-Guided Investigation Provides New Insights into Secondary Metabolites of  SX6 from 

Whole-genome sequencing and genome mining are recently considered an efficient approach to shine more light on the underlying secondary metabolites of...

Genome-Guided Investigation Provides New Insights into Secondary Metabolites of Streptomyces parvulus SX6 from Aegiceras corniculatum

Article Category: Original Paper

Data publikacji: 24 wrz 2022

Zakres stron: 381 - 394

Otrzymano: 16 mar 2022

Przyjęty: 13 lip 2022

DOI: https://doi.org/10.33073/pjm-2022-034

Słowa kluczowe
genome mining, plant-derived compounds, secondary metabolites

© 2022 Ngoc Tung Quach et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Genome-Guided Investigation Provides New Insights into Secondary Metabolites of Streptomyces parvulus SX6 from Aegiceras corniculatum

Article Category: Original Paper

Data publikacji: 24 wrz 2022

Zakres stron: 381 - 394

Otrzymano: 16 mar 2022

Przyjęty: 13 lip 2022

DOI: https://doi.org/10.33073/pjm-2022-034

Słowa kluczowegenome mining, plant-derived compounds, secondary metabolites

© 2022 Ngoc Tung Quach et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Słowa kluczowe
genome mining, plant-derived compounds, secondary metabolites