<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The oral cavity serves as another reservoir for gastric <italic>Helicobacter pylori</italic> and may contribute to the failure of gastric <italic>H. pylori</italic> eradication therapy. However, changes to the oral microbial composition after gastric <italic>H. pylori</italic> eradication therapy has not yet been identified. This study aims to dissect whether the oral microbiota is involved and which bacterium mediates the clinic failure in <italic>H. pylori</italic> eradication. In the present study, the oral microorganisms from patients who had received the gastric <italic>H. pylori</italic> eradication treatment were analyzed by a high-throughput 16S rRNA deep sequencing. We found that the β diversity and composition of oral microbiota were remarkably changed in the patients who had experienced successful gastric <italic>H. pylori</italic> eradication treatment (SE group) compared to the failure group (FE group). Significantly enriched families, including <italic>Prevotellaceae</italic>, <italic>Streptococcaceae</italic>, Caulobacteraceae, and Lactobacillaceae, were detected in the SE group. In contrast, the bacterial families, such as Weeksellaceae, Neisseriaceae, Peptostreptococcaceae, Spirochaetaceae, and Veillonellaceae, were abundantly expressed in the FE group. Five operational taxonomic units (OTUs) were positively correlated with DOB values, while two OTUs exhibited negative trends. These different enriched OTUs were extensively involved in the 20 metabolic pathways. These results suggest that a balanced environment in the oral microbiota contributes to <italic>H. pylori</italic> eradication and metabolic homeostasis in humans. Our data demonstrated that the changes in oral microbiota might contribute to the therapeutic effects of antibiotic therapy. Therefore, a different therapy on the detrimental oral microbiota will increase the therapeutic efficacy of antibiotics on <italic>H. pylori</italic> infection.</p>
<p><fig id="j_pjm-2022-020_fig_001a" position="float" fig-type="figure">
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2022-020_fig_001a.jpg"></graphic>
</fig></p>
</abstract>

The oral cavity serves as another reservoir for gastric Helicobacter pylori and may contribute to the failure of gastric H. pylori eradication therapy. However, changes to the oral microbial composition after gastric H. pylori eradication therapy has not yet been identified. This study aims to dissect whether the oral microbiota is involved and which bacterium mediates the clinic failure in H. pylori eradication. In the present study, the oral microorganisms from patients who had received the gastric H. pylori eradication treatment were analyzed by a high-throughput 16S rRNA deep sequencing. We found that the β diversity and composition of oral microbiota were remarkably changed in the patients who had experienced successful gastric H. pylori eradication treatment (SE group) compared to the failure group (FE group). Significantly enriched families, including Prevotellaceae, Streptococcaceae, Caulobacteraceae, and Lactobacillaceae, were detected in the SE group. In contrast, the bacterial families, such as Weeksellaceae, Neisseriaceae, Peptostreptococcaceae, Spirochaetaceae, and Veillonellaceae, were abundantly expressed in the FE group. Five operational taxonomic units (OTUs) were positively correlated with DOB values, while two OTUs exhibited negative trends. These different enriched OTUs were extensively involved in the 20 metabolic pathways. These results suggest that a balanced environment in the oral microbiota contributes to H. pylori eradication and metabolic homeostasis in humans. Our data demonstrated that the changes in oral microbiota might contribute to the therapeutic effects of antibiotic therapy. Therefore, a different therapy on the detrimental oral microbiota will increase the therapeutic efficacy of antibiotics on H. pylori infection.

Oral Microbiota, a Potential Determinant for the Treatment Efficacy of Gastric <italic>Helicobacter pylori</italic> Eradication in Humans

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Oral Microbiota, a Potential Determinant for the Treatment Efficacy of Gastric  Eradication in Humans

The oral cavity serves as another reservoir for gastric Helicobacter pylori and may contribute to the failure of gastric H. pylori eradication therapy....

Oral Microbiota, a Potential Determinant for the Treatment Efficacy of Gastric Helicobacter pylori Eradication in Humans

Article Category: Original Paper

Data publikacji: 31 maj 2022

Zakres stron: 227 - 239

Otrzymano: 05 sty 2022

Przyjęty: 22 mar 2022

DOI: https://doi.org/10.33073/pjm-2022-020

Słowa kluczowe
eradication, therapeutic effects, oral microbiota, sequencing

© 2022 Huixia Chen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Oral Microbiota, a Potential Determinant for the Treatment Efficacy of Gastric Helicobacter pylori Eradication in Humans

Article Category: Original Paper

Data publikacji: 31 maj 2022

Zakres stron: 227 - 239

Otrzymano: 05 sty 2022

Przyjęty: 22 mar 2022

DOI: https://doi.org/10.33073/pjm-2022-020

Słowa kluczoweeradication, therapeutic effects, oral microbiota, sequencing

© 2022 Huixia Chen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Słowa kluczowe
eradication, therapeutic effects, oral microbiota, sequencing