<abstract xmlns="http://www.w3.org/1999/xhtml">

In order to clarified characteristics and function of internalin G (inlG) in <italic>Listeria monocytogenes</italic> ATCC®19111 (1/2a) (LM), the immune protection of the inlG was evaluated in mice, the homologous recombination was used to construct <italic>inlG</italic> deletion strains, and their biological characteristics were studied by the transcriptomics analysis. As a result, the immunization of mice with the purified protein achieved a protective effect against bacterial infection. The deletion strain LM-AinlG was successfully constructed with genetic stability. The mouse infection test showed that the virulence of LM was decreased after the deletion of the <italic>inlG</italic> gene. The deletion strain showed enhanced adhesion to and invasion of Caco-2 cells. Compared to the wild strain, 18 genes were up-regulated, and 24 genes were down-regulated in the LM-AinlG. This study has laid a foundation for further research on the function of <italic>inlG</italic> and the pathogenesis of LM.
In this study, immunization of mice with the purified inlG protein achieved a protective effect against <italic>Listeria monocytogenes</italic> infection. The virulence of LM-ΔinlG was decreased by mouse infection. However, the adhesion and invasion ability to Caco-2 cell were enhanced. Compared to the wild strain, 18 genes were up-regulated, and 24 genes were down-regulated in the LM-ΔinlG. This study has laid a foundation for further study of the function of the inlG and the listeriosis.
<fig id="j_pjm-2022-009_fig_006" position="float" fig-type="figure"><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2022-009_fig_006.jpg"></graphic></fig>
</abstract>

In order to clarified characteristics and function of internalin G (inlG) in Listeria monocytogenes ATCC®19111 (1/2a) (LM), the immune protection of the inlG was evaluated in mice, the homologous recombination was used to construct inlG deletion strains, and their biological characteristics were studied by the transcriptomics analysis. As a result, the immunization of mice with the purified protein achieved a protective effect against bacterial infection. The deletion strain LM-AinlG was successfully constructed with genetic stability. The mouse infection test showed that the virulence of LM was decreased after the deletion of the inlG gene. The deletion strain showed enhanced adhesion to and invasion of Caco-2 cells. Compared to the wild strain, 18 genes were up-regulated, and 24 genes were down-regulated in the LM-AinlG. This study has laid a foundation for further research on the function of inlG and the pathogenesis of LM.
In this study, immunization of mice with the purified inlG protein achieved a protective effect against Listeria monocytogenes infection. The virulence of LM-ΔinlG was decreased by mouse infection. However, the adhesion and invasion ability to Caco-2 cell were enhanced. Compared to the wild strain, 18 genes were up-regulated, and 24 genes were down-regulated in the LM-ΔinlG. This study has laid a foundation for further study of the function of the inlG and the listeriosis.

The Characteristics and Function of Internalin G in <italic>Listeria monocytogenes</italic>

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Characteristics and Function of Internalin G in 

In order to clarified characteristics and function of internalin G (inlG) in Listeria monocytogenes ATCC®19111 (1/2a) (LM), the immune protection of the inlG...

The Characteristics and Function of Internalin G in Listeria monocytogenes

Article Category: Original Paper

Data publikacji: 30 mar 2022

Zakres stron: 63 - 71

Otrzymano: 22 lis 2021

Przyjęty: 10 lut 2022

DOI: https://doi.org/10.33073/pjm-2022-009

Słowa kluczowe
internalin G, immune protection, gene deletion, transcriptomics

© 2022 Huitian Gou et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Characteristics and Function of Internalin G in Listeria monocytogenes

Article Category: Original Paper

Data publikacji: 30 mar 2022

Zakres stron: 63 - 71

Otrzymano: 22 lis 2021

Przyjęty: 10 lut 2022

DOI: https://doi.org/10.33073/pjm-2022-009

Słowa kluczoweinternalin G, immune protection, gene deletion, transcriptomics

© 2022 Huitian Gou et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Słowa kluczowe
internalin G, immune protection, gene deletion, transcriptomics