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Identification of Genetic Variants of Human Papillomavirus in a Group of Mexican HIV/AIDS Patients and Their Possible Association with Cervical Cancer

FELIPE ORTIZ-GUTIÉRREZ
FELIPE ORTIZ-GUTIÉRREZ
, 
LILIA SÁNCHEZ-MINUTTI
LILIA SÁNCHEZ-MINUTTI
, 
JOSÉ F. MARTÍNEZ-HERRERA
JOSÉ F. MARTÍNEZ-HERRERA
, 
INDIANA D. TORRES-ESCOBAR
INDIANA D. TORRES-ESCOBAR
, 
ELIAS B. PEZZAT-SAID
ELIAS B. PEZZAT-SAID
, 
LUIS MÁRQUEZ-DOMÍNGUEZ
LUIS MÁRQUEZ-DOMÍNGUEZ
 oraz 
AMADO I. GRANDES-BLANCO
AMADO I. GRANDES-BLANCO
   | 20 gru 2021
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Article Category: original-paper
Data publikacji: 20 gru 2021
Zakres stron: 501 - 509
Otrzymano: 11 wrz 2021
Przyjęty: 16 lis 2021
DOI: https://doi.org/10.33073/pjm-2021-047
Słowa kluczowe
© 2021 Felipe Ortiz-Gutiérrez et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

HPV genotype frequency detected by PCR. Red bars – HR-HPV types, green bars – LR-HPV.
HPV genotype frequency detected by PCR. Red bars – HR-HPV types, green bars – LR-HPV.

Fig. 2.

Frequency of HPV types. a) Raw data, b) calculated percentage. Age was classified into three groups: < 45, 45–54, ≥ 55 years.
Frequency of HPV types. a) Raw data, b) calculated percentage. Age was classified into three groups: < 45, 45–54, ≥ 55 years.

Fig. 3.

Comparison of nucleotide sequences of the L1 gene of HPV51 from clinical samples. The figure shows nucleotide sequences of seven HPV51-positive clinical isolates. The reference sequence is at the top. The nucleotides from the clinical samples that were homologous to the reference sequence are shown as points. Capital letters indicate differences concerning the reference sequence. Sequence alignment was performed using ClustalW multiple alignment software v1.4.
Comparison of nucleotide sequences of the L1 gene of HPV51 from clinical samples. The figure shows nucleotide sequences of seven HPV51-positive clinical isolates. The reference sequence is at the top. The nucleotides from the clinical samples that were homologous to the reference sequence are shown as points. Capital letters indicate differences concerning the reference sequence. Sequence alignment was performed using ClustalW multiple alignment software v1.4.

Fig. 4.

Comparison of amino acid sequences of the L1 gene of HPV51 from clinical samples. The figure shows amino acids sequences of seven HPV51-positive clinical isolates. The reference sequence is at the top. The amino acids from the clinical samples that were homologous to the reference sequence are shown as points. Capital letters indicate differences with respect to the reference sequence. Sequence alignment was performed using ClustalW multiple alignment software v1.4.
Comparison of amino acid sequences of the L1 gene of HPV51 from clinical samples. The figure shows amino acids sequences of seven HPV51-positive clinical isolates. The reference sequence is at the top. The amino acids from the clinical samples that were homologous to the reference sequence are shown as points. Capital letters indicate differences with respect to the reference sequence. Sequence alignment was performed using ClustalW multiple alignment software v1.4.

Fig. 5.

The L1 monomer of HPV51. The I21L, E26R, I52L, V71G, and F72I mutations are highlighted in magenta.
The L1 monomer of HPV51. The I21L, E26R, I52L, V71G, and F72I mutations are highlighted in magenta.

Clinical characteristics of the patients.

Patient ageHIV viral load (copies/ml)CD4+ (cell/ml)Pap cytology1HPV type2
Group I3
45< 50744I and II90 (LR)
30< 50726I and IIND
235,840655I11 (LR)
30< 50602I and II97 (LR)
1790,800580I and II51 (HR)
42< 50521IND
44< 50506I66 (HR)
50400503I and II16 (HR)
35< 50485I16 (HR)
27< 50481I and II51 (HR)
37< 50410IV51 (HR)
42< 50405I11 (LR)
6057388I58 (HR)
29< 50380I and II70 (LR)
314,940364I and IIND
Group II3
35< 50317I and IIND
35< 50294IVND
241,170282I and IIND
30< 50280III54 (LR)
42< 50258I and II102 (LR)
49< 50255I and II6 (LR)
35< 50208I84 (LR)
32369180I51(HR)
3613,900175I and II51 (HR)
37< 50161III81 (LR)
2867160I and IIND
2474,620146I and IIND
51< 50132III51 (HR)
3773127IV81 (LR)
485560104I and II6 (LR)
43< 50103I and II86 (LR)
37ND102I56 (HR)
31< 5076III52 (HR)
23> 100,00070I and II6 (LR)
3482265III58 (HR)
6345163I and IIND
445,14060I and II51 (HR)
259520030I and II81 (LR)
41ND22III33(HR)
31> 100,00016I and II70 (LR)
eISSN:
2544-4646
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Microbiology and Virology
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