Chemical Components of Aqueous Extracts of  Fruits and Their Effects on The Transcriptome of

HONG PENG; YING-SI WANG; JIE WANG; SU-JUAN LI; TING-LI SUN; TONG LIU; QING-SHAN SHI; GANG ZHOU; XIAO-BAO XIE

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Chemical Components of Aqueous Extracts of Melia azedarach Fruits and Their Effects on The Transcriptome of Staphylococcus aureus

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| 29 paź 2021

Polish Journal of Microbiology

Tom 70 (2021): Zeszyt 4 (December 2021)

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Article Category: original-paper

Data publikacji: 29 paź 2021

Zakres stron: 447 - 459

Otrzymano: 20 cze 2021

Przyjęty: 15 wrz 2021

DOI: https://doi.org/10.33073/pjm-2021-041

Słowa kluczowe
fruits, differentially expressed genes, biofilms, UHPLC-MS/MS

© 2021 Hong Peng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The planktonic growth (A) and biofilm formation (B) of Staphylococcus aureus can be inhibited by aqueous extracts of Melia azedarach fruits in a dose-dependent manner

Effect of aqueous extracts of Melia azedarach fruits on planktonic growth (A) and biofilm formation (B) of Staphylococcus aureus ATCC 6538. S. aureus ATCC 6538 was cultured on LB media supplemented with different concentrations of aqueous extracts of chinaberry fruits at 37°C for 48 h. The S. aureus planktonic cells and biofilms stained with crystal violet were measured at OD600 and OD590, respectively, using a Multiskan GO plate reader.

Representative CLSM images of Staphylococcus aureus ATCC 6538 biofilms grown in the presence of different concentrations of aqueous extracts of Melia azedarach fruits and stained with SYTO9. The biofilms were cultivated on the surface of glass coverslips for 48 h at 37°C. The constructed biofilms were stained with the fluorescent dye SYTO9 and then observed under a Zeiss LSM 710 CLSM. Scale bar = 50 μm. CLSM, confocal laser scanning microscopy.

Histogram depiction of enriched Gene Ontology classification of the DEGs of Staphylococcus aureus ATCC 6538 treated with 0.25 g/ml aqueous extracts of Melia azedarach fruits. The results were categorized into three main categories of biological process, molecular function, and cellular component. The x-axis indicates the gene numbers in each subcategory. The top 10 subcategories in each main category are shown here.

Functional analysis of DEGs of Staphylococcus aureus ATCC 6538 treated with 0.25 g/ml aqueous extracts of Melia azedarach fruits based on the KEGG pathway. Among the top 20 pathways, the first two pathways, the ribosome (pae03010) and valine, leucine, and isoleucine biosynthesis (pae00290), were significantly enriched (padj < 0.05). The right y-axis represents the KEGG pathway. The x-axis exhibits the enrichment factor, which denotes the ratio of the DEG numbers to the annotated gene numbers enriched in a specific pathway. DEG, differentially expressed genes; KEGG, Kyoto Encyclopedia of Genes and Genomes.

HMDB classification annotation of the components of aqueous extracts of Melia azedarach fruits in both positive and negative ion modes. The x-axis represents metabolite numbers, and the y-axis represents annotated HMDB entries.

Lipidmaps classification annotation of components of aqueous extracts of Melia azedarach fruits in both positive and negative ion modes. The x-axis represents metabolite numbers, and the y-axis represents annotated Lipidmaps entries.

KEGG enrichment chart of the components of aqueous extraction of Melia azedarach fruits in both positive and negative ion modes. The x-axis represents metabolite numbers, and the y-axis represents enriched KEGG pathways. KEGG, Kyoto Encyclopedia of Genes and Genomes.

Identification results of the top 25 metabolites from aqueous extracts of Melia azedarach fruits in a positive mode.

No.	Compound_ID	Name	Formula	Molecular Weight	Retention Time (min)
1	Com_2_pos	D-Pyrrolidine-2-Carboxylic acid	C₅H₉NO₂	115.0634	1.382
2	Com_3_pos	DL-Arginine	C₆H₁₄N₄O₂	174.1115	1.425
3	Com_6_pos	Choline	C₅H₁₃NO	103.0999	1.275
4	Com_49_pos	L-Glutamic acid	C₅H₉NO₄	147.0529	1.434
5	Com_28_pos	Nicotinic acid	C₆H₅NO₂	123.0321	1.773
6	Com_44_pos	Perillartine	C₁₀H₁₅NO	165.1152	7.342
7	Com_30_pos	6-Hydroxynicotinic acid	C₆H₅NO₃	139.0268	1.988
8	Com_59_pos	Maltol	C₆H₆O₃	126.0318	5.271
9	Com_21_pos	Isoamylamine	C₅H₁₃N	87.10517	4.875
10	Com_75_pos	5-oxoproline	C₅H₇NO₃	129.0426	1.45
11	Com_71_pos	Ethyl 4-amino-2-(methylsulfanyl)-1,3-thiazole-5-carboxylate	C₇H₁₀N₂O₂S₂	218.019	1.265
12	Com_77_pos	DL-Tryptophan	C₁₁H₁₂N₂O₂	204.0897	6.801
13	Com_98_pos	Ecgonine methyl ester	C₁₀H₁₇NO₃	199.1206	8.349
14	Com_103_pos	Scopoletin	C₁₀H₈O₄	192.0421	8.708
15	Com_105_pos	Gamma-Aminobutyric acid	C₄H₉NO₂	103.0637	1.292
16	Com_85_pos	Kinetin	C₁₀H₉N₅O	237.0635	2.048
17	Com_104_pos	N-Acetyl-DL-serine	C₅H₉NO₄	147.0529	1.284
18	Com_116_pos	Pipecolinic acid	C₆H₁₁NO₂	129.079	1.734
19	Com_119_pos	2-Methylenesuccinic acid	C₅H₆O₄	130.0263	1.284
20	Com_168_pos	2-Picolinic acid	C₆H₅NO₂	123.0321	1.523
21	Com_165_pos	6-O-(2-Methylbutanoyl)-α-D-glucopyranosyl α-D-glucopyranoside	C₁₇H₃₀O₁₂	443.1997	8.133
22	Com_135_pos	(+/-)12(13)-DiHOME	C₁₈H₃₄O₄	296.2345	14.142
23	Com_171_pos	Corylifol A	C₂₅H₂₆O₄	390.1823	10.9
24	Com_78_pos	Oleoyl ethylamide	C₂₀H₃₉NO	309.3025	15.542
25	Com_114_pos	Cytidine	C₉H₁₃N₃O₅	243.0893	8.013

Quantification of biofilm topologies in the presence of different concentrations of aqueous extracts of Melia azedarach fruits.

Parameters	Control	0.25 g/ml	0.33 g/ml	0.50 g/ml	1.00 g/ml
Maximum thickness (μm)	10 ± 0.00^a	10 ± 0.00^a	10 ± 0.00^a	10 ± 0.00^a	10 ± 0.00^a
Average thickness (μm)	7.75 ± 0.10^a	6.68 ± 0.36^b	3.37 ± 0.17^c	2.11 ± 0.19^d	1.11 ± 0.03^e
Total biomass (μm³/μm²)	9.81 ± 1.78^a	6.97 ± 0.08^b	2.98 ± 0.25^c	1.40 ± 0.24^cd	0.22 ± 0.02^d

Identification results of the top 25 metabolites from aqueous extracts of Melia azedarach fruits in a negative mode.

No.	Compound_ID	Name	Formula	Molecular Weight	Retention Time (min)
1	Com_2_neg	D-Saccharic acid	C₆H₁₀O₈	210.0372	1.174
2	Com_6_neg	Palmitic acid	C₁₆H₃₂O₂	256.2397	14.6
3	Com_4_neg	Citric acid	C₆H₈O₇	192.0267	1.214
4	Com_11_neg	N-Acetylneuraminic acid	C₁₁H₁₉NO₉	309.1055	1.296
5	Com_17_neg	Elaidic acid	C₁₈H₃₄O₂	282.2553	14.704
6	Com_15_neg	Sucrose	C₁₂H₂₂O₁₁	402.137	1.39
7	Com_22_neg	D-(–)-Lyxose	C₅H₁₀O₅	150.0526	1.268
8	Com_21_neg	Gluconic acid	C₆H₁₂O₇	196.058	1.256
9	Com_29_neg	DL-Malic acid	C₄H₆O₅	134.0214	1.186
10	Com_23_neg	4-Oxoproline	C₅H₇NO₃	83.03709	1.293
11	Com_31_neg	Pyruvic acid	C₃H₄O₃	88.01595	1.186
12	Com_25_neg	D-(–)-Fructose	C₆H₁₂O₆	180.0632	1.307
13	Com_48_neg	Glutaconic acid	C₅H₆O₄	130.0265	1.172
14	Com_56_neg	2-Furoic acid	C₅H₄O₃	112.016	1.155
15	Com_47_neg	Toosendanin	C₃₀H₃₈O₁₁	574.2414	11.083
16	Com_57_neg	α-Eleostearic acid	C₁₈H₃₀O₂	278.2241	14.157
17	Com_50_neg	α-Lactose	C₁₂H₂₂O₁₁	388.1213	1.373
18	Com_71_neg	Nomilin	C₂₈H₃₄O₉	514.2193	10.413
19	Com_54_neg	4-Acetamidobutanoic acid	C₆H₁₁NO₃	145.0739	1.439
20	Com_59_neg	D-(+)-Glucose	C₆H₁₂O₆	180.0632	1.484
21	Com_70_neg	6-Sialyllactose	C₂₃H₃₉NO₁₉	633.2127	1.319
22	Com_78_neg	Purine	C₅H₄N₄	120.0422	1.274
23	Com_77_neg	2-C-methyl D-erythritol 4-phosphate	C₅H₁₃O₇P	216.0399	1.27
24	Com_72_neg	Azelaic acid	C₉H₁₆O₄	188.1047	5.304
25	Com_81_neg	16-Hydroxyhexadecanoic acid	C₁₆H₃₂O₃	254.2242	14.26

eISSN:: 2544-4646
Język:: Angielski

Częstotliwość wydawania:: 4 razy w roku
Dziedziny czasopisma:: Life Sciences, Microbiology and Virology

Kanał RSS czasopisma

Chemical Components of Aqueous Extracts of Melia azedarach Fruits and Their Effects on The Transcriptome of Staphylococcus aureus

Article Category: original-paper

Data publikacji: 29 paź 2021

Zakres stron: 447 - 459

Otrzymano: 20 cze 2021

Przyjęty: 15 wrz 2021

DOI: https://doi.org/10.33073/pjm-2021-041

Słowa kluczowe
fruits, differentially expressed genes, biofilms, UHPLC-MS/MS

© 2021 Hong Peng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

Identification results of the top 25 metabolites from aqueous extracts of Melia azedarach fruits in a positive mode.

Quantification of biofilm topologies in the presence of different concentrations of aqueous extracts of Melia azedarach fruits.

Identification results of the top 25 metabolites from aqueous extracts of Melia azedarach fruits in a negative mode.

Chemical Components of Aqueous Extracts of Melia azedarach Fruits and Their Effects on The Transcriptome of Staphylococcus aureus

Article Category: original-paper

Data publikacji: 29 paź 2021

Zakres stron: 447 - 459

Otrzymano: 20 cze 2021

Przyjęty: 15 wrz 2021

DOI: https://doi.org/10.33073/pjm-2021-041

Słowa kluczowefruits, differentially expressed genes, biofilms, UHPLC-MS/MS

© 2021 Hong Peng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

Identification results of the top 25 metabolites from aqueous extracts of Melia azedarach fruits in a positive mode.

Quantification of biofilm topologies in the presence of different concentrations of aqueous extracts of Melia azedarach fruits.

Identification results of the top 25 metabolites from aqueous extracts of Melia azedarach fruits in a negative mode.

Słowa kluczowe
fruits, differentially expressed genes, biofilms, UHPLC-MS/MS