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Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Glehnia littoralis Based on Illumina Sequencing

XIAOWEI HUO
XIAOWEI HUO
, 
YUE WANG
YUE WANG
, 
DAWEI ZHANG
DAWEI ZHANG
, 
TING GAO
TING GAO
 oraz 
MENGMENG LIU
MENGMENG LIU
   | 08 wrz 2020
Polish Journal of Microbiology's Cover Image
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Article Category: original-paper
Data publikacji: 08 wrz 2020
Zakres stron: 283 - 291
Otrzymano: 17 kwi 2020
Przyjęty: 19 cze 2020
DOI: https://doi.org/10.33073/pjm-2020-031
Słowa kluczowe
© 2020 Xiaowei Huo et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Rarefaction curves based on the Shannon index OUT level. Error bars represent the standard error of four replicates.
Rarefaction curves based on the Shannon index OUT level. Error bars represent the standard error of four replicates.

Fig. 2.

Venn diagram showing the OTUs shared among different samples associated with G. littoralis.
Venn diagram showing the OTUs shared among different samples associated with G. littoralis.

Fig. 3.

The bacterial abundance of different tissues at the phylum level.
The bacterial abundance of different tissues at the phylum level.

Fig. 4.

Heatmap displaying the relative abundances of the most dominant genera (top 50) in each sample. The dendrogram represents complete-linkage agglomerative clustering, based on Euclidean dissimilarities.
Heatmap displaying the relative abundances of the most dominant genera (top 50) in each sample. The dendrogram represents complete-linkage agglomerative clustering, based on Euclidean dissimilarities.

Fig. 5.

Statistical comparison of the relative abundance at the family level by the Kruskal-Wallis H test. P < 0.05 was considered statistically significant.
Statistical comparison of the relative abundance at the family level by the Kruskal-Wallis H test. P < 0.05 was considered statistically significant.

Fig. 6.

Hierarchical cluster analysis of different microbiota in different samples using pairwise weighted UniFrac distances. L1, L2, L3, L4, four repetitions of the leaf; S1, S2, S3, S4, four repetitions of the stem; R1, R2, R3, R4, four repetitions of the root.
Hierarchical cluster analysis of different microbiota in different samples using pairwise weighted UniFrac distances. L1, L2, L3, L4, four repetitions of the leaf; S1, S2, S3, S4, four repetitions of the stem; R1, R2, R3, R4, four repetitions of the root.

Fig. 7.

Principal coordinate analysis (PCoA) based on Bray-Curtis dissimilarity between different samples.
Principal coordinate analysis (PCoA) based on Bray-Curtis dissimilarity between different samples.

Characteristics of effective tags from samples of endophytic bacteria and rhizosphere bacteria associated with G. littoralis.

SampleSample siteNumber of tagsTotal length (bp)Average length (bp)Effective (%)
Leaf1161,49124,250,81639486.74
Leaf2270,53927,826,64739487.38
Leaf3372,50228,614,92639480.78
Leaf4456,67922,401,39339571.51
Stem1174,17629,259,67039485.41
Stem2271,88328,374,40839479.27
Stem3373,23228,877,13839466.46
Stem4468,97127,201,00639481.54
Root1174,70529,478,19139479.50
Root2270,45627,847,10539570.56
Root3353,18321,019,55039572.88
Root4472,01728,464,45939573.40

Operational taxonomic unit (OTU) richness and diversity indices of different samples associated with G. littoralis with a 97% similarity cut-off.

Sample nameOTUs observedShannonChao1ACECoverage (%)
Leaf526 ± 34 b3.58 ± 0.43 b600 ± 66 b599 ± 69 b99.6
Steam555 ± 22 b3.73 ± 0.39 b613 ± 82 b616 ± 77 b99.6
Root694 ± 19 a4.60 ± 0.19 a803 ± 40 a818 ± 42 a99.6

The Adonis analysis of the difference among samples associated with G. littoralis.

TissuesLeafStem
RootR2 = 0.68, p = 0.027R2 = 0.46, p = 0.041
StemR2 = 0.09, p = 0.748
eISSN:
2544-4646
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Microbiology and Virology
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