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New Look on Antifungal Activity of Silver Nanoparticles (AgNPs)

BARBARA ŻAROWSKA

BARBARA ŻAROWSKA

Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life SciencesWrocław, Poland
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ŻAROWSKA, BARBARA
, 
TOMASZ KOŹLECKI

TOMASZ KOŹLECKI

Department of Chemical Engineering, Wrocław University of TechnologyWrocław, Poland
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KOŹLECKI, TOMASZ
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MICHAŁ PIEGZA

MICHAŁ PIEGZA

Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life SciencesWrocław, Poland
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PIEGZA, MICHAŁ
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KATARZYNA JAROS-KOŹLECKA

KATARZYNA JAROS-KOŹLECKA

TK NanoWrocław, Poland
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JAROS-KOŹLECKA, KATARZYNA
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MAŁGORZATA ROBAK

MAŁGORZATA ROBAK

Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life SciencesWrocław, Poland
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ROBAK, MAŁGORZATA
  
05 gru 2019
New Look on Antifungal Activity of Silver Nanoparticles (AgNPs)'s Cover Image
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Kategoria artykułu: original-paper
Data publikacji: 05 gru 2019
Zakres stron: 515 - 525
Otrzymano: 12 cze 2019
Przyjęty: 19 paź 2019
DOI: https://doi.org/10.33073/pjm-2019-051
Słowa kluczowe
© 2019 Barbara Żarowska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Nanoparticles’ shape (A) and dimensions (B).
Nanoparticles’ shape (A) and dimensions (B).

Fig. 2.

Microorganism growth in BioscreenC with and without AgNPs: (A) Bacteria: S.a, Staphylococcus aureus; P.a, Pseudomonas aeruginosa; E.c, Escherichia coli; (B) yeasts Y.l, Yarrowia lipolytica, and fungi: Smix, the mixture of spores of five species of filamentous fungi; A.b, Aspergillus brasiliensis; P.p, Penicillium pinophilum; P.v, Paecilomyces variotii; T.v, Trichoderma virens, and Ch.g, Chaetomium globosum.
Microorganism growth in BioscreenC with and without AgNPs: (A) Bacteria: S.a, Staphylococcus aureus; P.a, Pseudomonas aeruginosa; E.c, Escherichia coli; (B) yeasts Y.l, Yarrowia lipolytica, and fungi: Smix, the mixture of spores of five species of filamentous fungi; A.b, Aspergillus brasiliensis; P.p, Penicillium pinophilum; P.v, Paecilomyces variotii; T.v, Trichoderma virens, and Ch.g, Chaetomium globosum.

Fig. 3.

TEM images of Aspergillus brasiliensis after the growth in the BioscreenC: (a) without AgNPs; (b) with AgNPs visible inside the cell (black points); (c, d) accumulated within the cell wall.
TEM images of Aspergillus brasiliensis after the growth in the BioscreenC: (a) without AgNPs; (b) with AgNPs visible inside the cell (black points); (c, d) accumulated within the cell wall.

Fig. 4.

TEM images of filamentous fungi after the growth with AgNPs in the Bioscreen C: (a, b) Paecilomyces variotii; (c, d) Penicillium pinophilum; AgNPs, the black structures observed inside the cell and hypha.
TEM images of filamentous fungi after the growth with AgNPs in the Bioscreen C: (a, b) Paecilomyces variotii; (c, d) Penicillium pinophilum; AgNPs, the black structures observed inside the cell and hypha.

Fig. 5.

TEM images of the bacterium and yeasts after the growth in the BioscreenC: (a) Pseudomonas aeruginosa without AgNPs; (b) P. aeruginosa with AgNPs; (c, d) Candida albicans with AgNPs, the black structures visible inside and outside of cells.
TEM images of the bacterium and yeasts after the growth in the BioscreenC: (a) Pseudomonas aeruginosa without AgNPs; (b) P. aeruginosa with AgNPs; (c, d) Candida albicans with AgNPs, the black structures visible inside and outside of cells.

Lag phase duration and final OD after 96 hours of filamentous fungal and yeast growth in BioscreenC without or with AgNPs_

Filamentous fungi and yeast speciesLag phase [h]OD
at 96 hcompared to
SM [%]initial [× folds]
SM: spores mix (control)101.59210036
SM: spores mix +AgNPs201.308825.8
A. brasiliensis +AgNPs401.6001008.1
P. pinophilum +AgNPs301.517959.2
P. variotii +AgNPs~ 680.633403.7
T. virens +AgNPs181.306826.5
C. globosum +AgNPs481.063675.2
C. albicans +AgNPs150.953–3.8
Y. lipolytica +AgNPs> 960.194–0

AgNP inhibition of the filamentous fungi growth_

Fungal speciesAgNPs in the medium [mg/l]
02.144.2821.4
Growth [%]
Aspergillus brasiliensis10010083.13.74
Trichoderma virens10010033.99.72
Paecilomyces variotii10010000
Penicillium pinophilum10057.344.80
Chaetomium globosum10063.961.20

The size and distribution (inside, outside, and within the cell wall) of AgNPs after their interaction with microorganisms_

AgNPs Size [nm][%]Cell distribution according o the size [%]
InsideOutsideIn the cell wallTotal
21.45 ± 5.979.137.451.611100
54.49 ± 9.113.993.86.250100
94.62 ± 18.167.085.714.30100
Total10048.742.68.7100
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Nauki biologiczne, Mikrobiologia i wirusologia
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