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New Look on Antifungal Activity of Silver Nanoparticles (AgNPs)

BARBARA ŻAROWSKA
BARBARA ŻAROWSKA
, 
TOMASZ KOŹLECKI
TOMASZ KOŹLECKI
, 
MICHAŁ PIEGZA
MICHAŁ PIEGZA
, 
KATARZYNA JAROS-KOŹLECKA
KATARZYNA JAROS-KOŹLECKA
 oraz 
MAŁGORZATA ROBAK
MAŁGORZATA ROBAK
   | 05 gru 2019
Polish Journal of Microbiology's Cover Image
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Article Category: original-paper
Data publikacji: 05 gru 2019
Zakres stron: 515 - 525
Otrzymano: 12 cze 2019
Przyjęty: 19 paź 2019
DOI: https://doi.org/10.33073/pjm-2019-051
Słowa kluczowe
© 2019 Barbara Żarowska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Nanoparticles’ shape (A) and dimensions (B).
Nanoparticles’ shape (A) and dimensions (B).

Fig. 2.

Microorganism growth in BioscreenC with and without AgNPs: (A) Bacteria: S.a, Staphylococcus aureus; P.a, Pseudomonas aeruginosa; E.c, Escherichia coli; (B) yeasts Y.l, Yarrowia lipolytica, and fungi: Smix, the mixture of spores of five species of filamentous fungi; A.b, Aspergillus brasiliensis; P.p, Penicillium pinophilum; P.v, Paecilomyces variotii; T.v, Trichoderma virens, and Ch.g, Chaetomium globosum.
Microorganism growth in BioscreenC with and without AgNPs: (A) Bacteria: S.a, Staphylococcus aureus; P.a, Pseudomonas aeruginosa; E.c, Escherichia coli; (B) yeasts Y.l, Yarrowia lipolytica, and fungi: Smix, the mixture of spores of five species of filamentous fungi; A.b, Aspergillus brasiliensis; P.p, Penicillium pinophilum; P.v, Paecilomyces variotii; T.v, Trichoderma virens, and Ch.g, Chaetomium globosum.

Fig. 3.

TEM images of Aspergillus brasiliensis after the growth in the BioscreenC: (a) without AgNPs; (b) with AgNPs visible inside the cell (black points); (c, d) accumulated within the cell wall.
TEM images of Aspergillus brasiliensis after the growth in the BioscreenC: (a) without AgNPs; (b) with AgNPs visible inside the cell (black points); (c, d) accumulated within the cell wall.

Fig. 4.

TEM images of filamentous fungi after the growth with AgNPs in the Bioscreen C: (a, b) Paecilomyces variotii; (c, d) Penicillium pinophilum; AgNPs, the black structures observed inside the cell and hypha.
TEM images of filamentous fungi after the growth with AgNPs in the Bioscreen C: (a, b) Paecilomyces variotii; (c, d) Penicillium pinophilum; AgNPs, the black structures observed inside the cell and hypha.

Fig. 5.

TEM images of the bacterium and yeasts after the growth in the BioscreenC: (a) Pseudomonas aeruginosa without AgNPs; (b) P. aeruginosa with AgNPs; (c, d) Candida albicans with AgNPs, the black structures visible inside and outside of cells.
TEM images of the bacterium and yeasts after the growth in the BioscreenC: (a) Pseudomonas aeruginosa without AgNPs; (b) P. aeruginosa with AgNPs; (c, d) Candida albicans with AgNPs, the black structures visible inside and outside of cells.

Lag phase duration and final OD after 96 hours of filamentous fungal and yeast growth in BioscreenC without or with AgNPs.

Filamentous fungi and yeast speciesLag phase [h]OD
at 96 hcompared to
SM [%]initial [× folds]
SM: spores mix (control)101.59210036
SM: spores mix +AgNPs201.308825.8
A. brasiliensis +AgNPs401.6001008.1
P. pinophilum +AgNPs301.517959.2
P. variotii +AgNPs~ 680.633403.7
T. virens +AgNPs181.306826.5
C. globosum +AgNPs481.063675.2
C. albicans +AgNPs150.953–3.8
Y. lipolytica +AgNPs> 960.194–0

AgNP inhibition of the filamentous fungi growth.

Fungal speciesAgNPs in the medium [mg/l]
02.144.2821.4
Growth [%]
Aspergillus brasiliensis10010083.13.74
Trichoderma virens10010033.99.72
Paecilomyces variotii10010000
Penicillium pinophilum10057.344.80
Chaetomium globosum10063.961.20

The size and distribution (inside, outside, and within the cell wall) of AgNPs after their interaction with microorganisms.

AgNPs Size [nm][%]Cell distribution according o the size [%]
InsideOutsideIn the cell wallTotal
21.45 ± 5.979.137.451.611100
54.49 ± 9.113.993.86.250100
94.62 ± 18.167.085.714.30100
Total10048.742.68.7100
eISSN:
2544-4646
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Microbiology and Virology
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