<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Penaeus monodon</italic> is highly susceptible to vibriosis disease. Aims of the study were to identify the pathogen causing vibriosis in <italic>P. monodon</italic> through molecular techniques and develop a biocontrol method of the disease by application of herbal extracts. Shrimp samples were collected aseptically from the infected farm and the bacteria were isolated from the infected region of those samples. Based on phenotypic identification, several isolates were identified as <italic>Vibrio</italic> sp. 16S rRNA gene sequences of the selected isolates exhibited 100% homology with <italic>V. alginolyticus</italic> strain ATCC 17749. An <italic>in vivo</italic> infection challenge test was performed by immersion method with <italic>V. alginolyticus</italic> where these isolates caused high mortality in juvenile shrimp with prominent symptoms of hepatopancreatic necrosis. Antibiogram profile of the isolates was determined against eleven commercial antibiotic discs whereas the isolates were found resistant to multiple antibiotics. A total of twenty-one herbal extracts were screened where <italic>Emblica officinalis, Allium sativum</italic>, and <italic>Syzygium aromaticum</italic> strongly inhibited the growth of <italic>V. alginolyticus</italic> in <italic>in vitro</italic> conditions. In <italic>in vivo</italic> conditions, the ethyl acetate extracts of <italic>E. officinalis</italic> and <italic>A. sativum</italic> successfully controlled the vibriosis disease in shrimp at a dose of 10 mg/g feed. This is the first report on molecular identification and biocontrol of <italic>V. alginolyticus</italic> in shrimp in Bangladesh.</p></abstract>

Penaeus monodon is highly susceptible to vibriosis disease. Aims of the study were to identify the pathogen causing vibriosis in P. monodon through molecular techniques and develop a biocontrol method of the disease by application of herbal extracts. Shrimp samples were collected aseptically from the infected farm and the bacteria were isolated from the infected region of those samples. Based on phenotypic identification, several isolates were identified as Vibrio sp. 16S rRNA gene sequences of the selected isolates exhibited 100% homology with V. alginolyticus strain ATCC 17749. An in vivo infection challenge test was performed by immersion method with V. alginolyticus where these isolates caused high mortality in juvenile shrimp with prominent symptoms of hepatopancreatic necrosis. Antibiogram profile of the isolates was determined against eleven commercial antibiotic discs whereas the isolates were found resistant to multiple antibiotics. A total of twenty-one herbal extracts were screened where Emblica officinalis, Allium sativum, and Syzygium aromaticum strongly inhibited the growth of V. alginolyticus in in vitro conditions. In in vivo conditions, the ethyl acetate extracts of E. officinalis and A. sativum successfully controlled the vibriosis disease in shrimp at a dose of 10 mg/g feed. This is the first report on molecular identification and biocontrol of V. alginolyticus in shrimp in Bangladesh.

Molecular Identification of <italic>Vibrio alginolyticus</italic> Causing Vibriosis in Shrimp and Its Herbal Remedy

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Molecular Identification of  Causing Vibriosis in Shrimp and Its Herbal Remedy

Penaeus monodon is highly susceptible to vibriosis disease. Aims of the study were to identify the pathogen causing vibriosis in P. monodon through molecular...

Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy

Article Category: original-paper

Data publikacji: 31 paź 2019

Zakres stron: 429 - 438

Otrzymano: 19 kwi 2019

Przyjęty: 05 sie 2019

DOI: https://doi.org/10.33073/pjm-2019-042

Słowa kluczowe
hepatopancreatic necrosis, challenge test,

© 2019 Marta Kłos et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy

Article Category: original-paper

Data publikacji: 31 paź 2019

Zakres stron: 429 - 438

Otrzymano: 19 kwi 2019

Przyjęty: 05 sie 2019

DOI: https://doi.org/10.33073/pjm-2019-042

Słowa kluczowehepatopancreatic necrosis, challenge test,

© 2019 Marta Kłos et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Słowa kluczowe
hepatopancreatic necrosis, challenge test,