Evaluation of three common methods of bulk lipid quantification in soft tissues of marine benthic invertebrates

Abele, D., & Puntarulo, S. (2004). Formation of reactive species and induction of antioxidant defence systems in polar and temperate marine invertebrates and fish. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 138(4), 405–415. https://doi.org/10.1016/j.cbpb.2004.05.013 Abele D. Puntarulo S. ( 2004 ). Formation of reactive species and induction of antioxidant defence systems in polar and temperate marine invertebrates and fish . Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology , 138 ( 4 ), 405 – 415 . https://doi.org/10.1016/j.cbpb.2004.05.013 Search in Google Scholar

Acevedo-Whitehouse, K., & Duffus, A. L. J. (2009). Effects of environmental change on wildlife health. Philosophical Transactions of the Royal Society B: Biological Sciences, 364(1534), 3429–3438. https://doi.org/10.1098/rstb.2009.0128 Acevedo-Whitehouse K. Duffus A. L. J. 1 ( 2009 ). Effects of environmental change on wildlife health . Philosophical Transactions of the Royal Society B: Biological Sciences , 364 ( 1534 ), 3429 – 3438 . https://doi.org/10.1098/rstb.2009.0128 Search in Google Scholar

Anglade, I., Dahl, T. H., Kristensen, B. S. B., Hagemann, A., Malzahn, A. M., & Reitan, K. I. (2023). Biochemical composition of Hediste diversicolor (OF Müller, 1776) (Annelida: Nereidae) reared on different types of aquaculture sludge. Frontiers in Marine Science, 10, 1197052. https://doi.org/10.3389/fmars.2023.1197052 Anglade I. Dahl T. H. Kristensen B. S. B. Hagemann A. Malzahn A. M. Reitan K. I. ( 2023 ). Biochemical composition of Hediste diversicolor (OF Müller, 1776) (Annelida: Nereidae) reared on different types of aquaculture sludge . Frontiers in Marine Science , 10 , 1197052 . https://doi.org/10.3389/fmars.2023.1197052 Search in Google Scholar

Araujo, P. (2009). Key aspects of analytical method validation and linearity evaluation. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877(23), 2224–2234. https://doi.org/10.1016/j. jchromb.2008.09.030 Araujo P. ( 2009 ). Key aspects of analytical method validation and linearity evaluation . Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , 877 ( 23 ), 2224 – 2234 . https://doi.org/10.1016/j.jchromb.2008.09.030 Search in Google Scholar

Barthel, D. (1986). On the ecophysiology of the sponge Halichondria panicea in Kiel Bight. I. Substrate specificity, growth and reproduction. Marine Ecology Progress Series, 32(1/2), 291–298. https://doi.org/10.3354/meps032291 Barthel D. ( 1986 ). On the ecophysiology of the sponge Halichondria panicea in Kiel Bight. I. Substrate specificity, growth and reproduction . Marine Ecology Progress Series , 32 ( 1/2 ), 291 – 298 . https://doi.org/10.3354/meps032291 Search in Google Scholar

Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911–917. https://doi. org/10.1139/o59-099 Bligh E. G. Dyer W. J. ( 1959 ). A rapid method of total lipid extraction and purification . Canadian Journal of Biochemistry and Physiology , 37 ( 8 ), 911 – 917 . https://doi.org/10.1139/o59-099 Search in Google Scholar

Christian, G. D. (2009). Herramientas y operaciones básicas de la química analítica. In G. D. Christian (Ed.), Química Analítica (pp. 20–64). McGraw-Hill Education. (in Spanish). Christian G. D. ( 2009 ). Herramientas y operaciones básicas de la química analítica . In Christian G. D. (Ed.), Química Analítica (pp. 20 – 64 ). McGraw-Hill Education . (in Spanish). Search in Google Scholar

da Costa, F., Nóvoa, S., Ojea, J., & Martínez-Patiño, D. (2013). Biochemical and fatty acid dynamics during larval development in the razor clam Ensis arcuatus (Bivalvia: Pharidae). Aquaculture Research, 44(12), 1926–1939. https://doi.org/10.1111/j.1365-2109.2012.03197.x da Costa F. Nóvoa S. Ojea J. Martínez-Patiño D. ( 2013 ). Biochemical and fatty acid dynamics during larval development in the razor clam Ensis arcuatus (Bivalvia: Pharidae) . Aquaculture Research , 44 ( 12 ), 1926 – 1939 . https://doi.org/10.1111/j.1365-2109.2012.03197.x Search in Google Scholar

da Silva-Castiglioni, D., Oliveira, G. T., & Buckup, L. (2010). Metabolic responses of Parastacus defossus and Parastacus brasiliensis (Crustacea, Decapoda, Parastacidae) to hypoxia. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 156(4), 436–444. https://doi.org/10.1016/j.cbpa.2010.03.025 da Silva-Castiglioni D. Oliveira G. T. Buckup L. ( 2010 ). Metabolic responses of Parastacus defossus and Parastacus brasiliensis (Crustacea, Decapoda, Parastacidae) to hypoxia . Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology , 156 ( 4 ), 436 – 444 . https://doi.org/10.1016/j.cbpa.2010.03.025 Search in Google Scholar

Dong-Young, L., Jung Hyun, K., Tae Hee, P., Hee Yoon, K., Dongyoung, K., Sungmin, H., & Hyun Je, P. (2022). Seasonal variations in biochemical and stable isotope compositions of particulate organic matter in two contrasting temperate coastal lagoons of Korea. Frontiers in Marine Science, 9, 953648. https://doi.org/10.3389/fmars.2022.953648 Dong-Young L. Jung Hyun K. Tae Hee P. Hee Yoon K. Dongyoung K. Sungmin H. Hyun Je P. ( 2022 ). Seasonal variations in biochemical and stable isotope compositions of particulate organic matter in two contrasting temperate coastal lagoons of Korea . Frontiers in Marine Science , 9 , 953648 . https://doi.org/10.3389/fmars.2022.953648 Search in Google Scholar

Friedrich, C., & Hagen, W. (1994). Lipid contents of five species of notothenioid fish from high-Antarctic waters and ecological implications. Polar Biology, 14, 359–369. https://doi.org/10.1007/BF00240256 Friedrich C. Hagen W. ( 1994 ). Lipid contents of five species of notothenioid fish from high-Antarctic waters and ecological implications . Polar Biology , 14 , 359 – 369 . https://doi.org/10.1007/BF00240256 Search in Google Scholar

Frings, C. S., & Dunn, R. T. (1970). A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction. American Journal of Clinical Pathology, 53(1), 89–91. https://doi.org/10.1093/ajcp/53.1.89 Frings C. S. Dunn R. T. ( 1970 ). A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction . American Journal of Clinical Pathology , 53 ( 1 ), 89 – 91 . https://doi.org/10.1093/ajcp/53.1.89 Search in Google Scholar

García-Alonso, J., Müller, C. T., & Hardege, J. D. (2008). Influence of food regimes and seasonality on fatty acid composition in the ragworm. Aquatic Biology, 4(1), 7–13. https://doi. org/10.3354/ab00090 García-Alonso J. Müller C. T. Hardege J. D. ( 2008 ). Influence of food regimes and seasonality on fatty acid composition in the ragworm . Aquatic Biology , 4 ( 1 ), 7 – 13 . https://doi.org/10.3354/ab00090 Search in Google Scholar

Gardner, W. S., Frez, W. A., Cichocki, E. A., & Parrish, C. C. (1985). Micromethod for lipids in aquatic invertebrates. Limnology and Oceanography, 30(5), 1099–1105. https://doi.org/10.4319/lo.1985.30.5.1099 Gardner W. S. Frez W. A. Cichocki E. A. Parrish C. C. ( 1985 ). Micromethod for lipids in aquatic invertebrates . Limnology and Oceanography , 30 ( 5 ), 1099 – 1105 . https://doi.org/10.4319/lo.1985.30.5.1099 Search in Google Scholar

González, F. J. E., Hernández Torres, M. E., Frenich, A. G., Martínez Vidal, J. L., & Campaña, A. M. G. (2004). Internal qualitycontrol and laboratory-management tools for enhancing the stability of results in pesticide multi-residue analytical methods. Trends in Analytical Chemistry, 23(5), 361–369. https://doi.org/10.1016/S0165-9936(04)00520-5 González F. J. E. Hernández Torres M. E. Frenich A. G. Martínez Vidal J. L. Campaña A. M. G. ( 2004 ). Internal qualitycontrol and laboratory-management tools for enhancing the stability of results in pesticide multi-residue analytical methods . Trends in Analytical Chemistry , 23 ( 5 ), 361 – 369 . https://doi.org/10.1016/S0165-9936(04)00520-5 Search in Google Scholar

Gora, A., Jayasankar, V., Rehman, S., Kizhakudan, J. K., Laxmilatha, P., & Vijayagopal, P. (2018). Biochemical responses of juvenile rock spiny lobster Panulirus homarus under different feeding regimes. Journal of Applied Animal Research, 46(1), 1462–1468. https://doi.org/10.1080/0971 2119.2018.1533475 Gora A. Jayasankar V. Rehman S. Kizhakudan J. K. Laxmilatha P. Vijayagopal P. ( 2018 ). Biochemical responses of juvenile rock spiny lobster Panulirus homarus under different feeding regimes . Journal of Applied Animal Research , 46 ( 1 ), 1462 – 1468 . https://doi.org/10.1080/09712119.2018.1533475 Search in Google Scholar

Graham, Z. A., de Jesus Florentino, J., Smithers, S. P., Menezes, J. C. T., de Carvalho, J. E., & Palaoro, A. V. (2024). Claw coloration in the fiddler crab Leptuca uruguayensis has no correlation with male quality. Current Zoology, 71(1), 109–123. https://doi.org/10.1093/cz/zoae035 Graham Z. A. de Jesus Florentino J. Smithers S. P. Menezes J. C. T. de Carvalho J. E. Palaoro A. V. ( 2024 ). Claw coloration in the fiddler crab Leptuca uruguayensis has no correlation with male quality . Current Zoology , 71 ( 1 ), 109 – 123 . https://doi.org/10.1093/cz/zoae035 Search in Google Scholar

Harvey, D. (2000). The language of analytical chemistry. In D. Harvey (Ed.), Modern analytical chemistry (pp. 35–52). McGraw-Hill Higher Education. Harvey D. ( 2000 ). The language of analytical chemistry . In Harvey D. (Ed.), Modern analytical chemistry (pp. 35 – 52 ). McGraw-Hill Higher Education . Search in Google Scholar

Hines, A., Yeung, W. H., Craft, J., Brown, M., Kennedy, J., Bignell, J., Stentiford, G. D., & Viant, M. R. (2007). Comparison of histological, genetic, metabolomics, and lipid-based methods for sex determination in marine mussels. Analytical Biochemistry, 369(2), 175–186. https://doi. org/10.1016/j.ab.2007.06.008 Hines A. Yeung W. H. Craft J. Brown M. Kennedy J. Bignell J. Stentiford G. D. Viant M. R. ( 2007 ). Comparison of histological, genetic, metabolomics, and lipid-based methods for sex determination in marine mussels . Analytical Biochemistry , 369 ( 2 ), 175 – 186 . https://doi.org/10.1016/j.ab.2007.06.008 Search in Google Scholar

Imbs, A. B., Ermolenko, E. V., Grigorchuk, V. P., Sikorskaya, T. V., & Velansky, P. V. (2021). Current progress in lipidomics of marine invertebrates. Marine Drugs, 19(12), 660. https://doi.org/10.3390/md19120660 Imbs A. B. Ermolenko E. V. Grigorchuk V. P. Sikorskaya T. V. Velansky P. V. ( 2021 ). Current progress in lipidomics of marine invertebrates . Marine Drugs , 19 ( 12 ), 660 . https://doi.org/10.3390/md19120660 Search in Google Scholar

Inouye, L. S., & Lotufo, G. R. (2006). Comparison of macro-gravimetric and micro-colorimetric lipid determination methods. Talanta, 70(3), 584–587. https://doi.org/10.1016/j.talanta.2006.01.024 Inouye L. S. Lotufo G. R. ( 2006 ). Comparison of macro-gravimetric and micro-colorimetric lipid determination methods . Talanta , 70 ( 3 ), 584 – 587 . https://doi.org/10.1016/j.talanta.2006.01.024 Search in Google Scholar

Jacobs, S. L., & Henry, R. J. (1962). Studies on the gravimetric determination of serum lipids. Clinica Chimica Acta, 7(2), 270–276. https://doi.org/10.1016/0009-8981(62)90020-7 Jacobs S. L. Henry R. J. ( 1962 ). Studies on the gravimetric determination of serum lipids . Clinica Chimica Acta , 7 ( 2 ), 270 – 276 . https://doi.org/10.1016/0009-8981(62)90020-7 Search in Google Scholar

Koutsouveli, V., Balgoma, D., Checa, A., Hedeland, M., Riesgo, A., & Cárdenas, P. (2022). Oogenesis and lipid metabolism in the deep-sea sponge Phakellia ventilabrum (Linnaeus, 1767). Scientific Reports, 12(1), 6317. https://doi. org/10.1038/s41598-022-10058-6 Koutsouveli V. Balgoma D. Checa A. Hedeland M. Riesgo A. Cárdenas P. ( 2022 ). Oogenesis and lipid metabolism in the deep-sea sponge Phakellia ventilabrum (Linnaeus, 1767) . Scientific Reports , 12 ( 1 ), 6317 . https://doi.org/10.1038/s41598-022-10058-6 Search in Google Scholar

Lasota, R., Sokołowski, A., Smolarz, K., Sromek, L., & Dublinowska, M. (2018). Multimarker response to salinity stress in two estuarine bivalves of different genetic diversity: Mya arenaria and Limecola balthica from the Gulf of Gdańsk (southern Baltic Sea). Invertebrate Biology, 137(3), 250–263. https://doi.org/10.1111/ivb.12224 Lasota R. Sokołowski A. Smolarz K. Sromek L. Dublinowska M. ( 2018 ). Multimarker response to salinity stress in two estuarine bivalves of different genetic diversity: Mya arenaria and Limecola balthica from the Gulf of Gdańsk (southern Baltic Sea) . Invertebrate Biology , 137 ( 3 ), 250 – 263 . https://doi.org/10.1111/ivb.12224 Search in Google Scholar

Lee, R. F., Hagen, W., & Kattner, G. (2006). Lipid storage in marine zooplankton. Marine Ecology Progress Series, 307, 273–306. https://doi.org/10.3354/meps307273 Lee R. F. Hagen W. Kattner G. ( 2006 ). Lipid storage in marine zooplankton . Marine Ecology Progress Series , 307 , 273 – 306 . https://doi.org/10.3354/meps307273 Search in Google Scholar

Lehtonen, K. K. (1996). Ecophysiology of the benthic amphipod Monoporeia affinis in an open-sea area of the northern Baltic Sea: Seasonal variations in body composition, with bioenergetic considerations. Marine Ecology Progress Series, 143, 87–98. https://doi.org/10.3354/meps143087 Lehtonen K. K. ( 1996 ). Ecophysiology of the benthic amphipod Monoporeia affinis in an open-sea area of the northern Baltic Sea: Seasonal variations in body composition, with bioenergetic considerations . Marine Ecology Progress Series , 143 , 87 – 98 . https://doi.org/10.3354/meps143087 Search in Google Scholar

Lehtonen, K. K. (2004). Seasonal variations in the physiological condition of the benthic amphipods Monoporeia affinis and Pontoporeia femorata in the Gulf of Riga (Baltic Sea). Aquatic Ecology, 38(3), 441–456. https://doi.org/10.1023/B:AECO.0000035165.97619.78 Lehtonen K. K. ( 2004 ). Seasonal variations in the physiological condition of the benthic amphipods Monoporeia affinis and Pontoporeia femorata in the Gulf of Riga (Baltic Sea) . Aquatic Ecology , 38 ( 3 ), 441 – 456 . https://doi.org/10.1023/B:AECO.0000035165.97619.78 Search in Google Scholar

Lehtonen, K. K., & Andersin, A. B. (1998). Population dynamics, response to sedimentation and role in benthic metabolism of the amphipod Monoporeia affinis in an open-sea area of the northern Baltic Sea. Marine Ecology Progress Series, 168, 71–85. https://doi.org/10.3354/meps168071 Lehtonen K. K. Andersin A. B. ( 1998 ). Population dynamics, response to sedimentation and role in benthic metabolism of the amphipod Monoporeia affinis in an open-sea area of the northern Baltic Sea . Marine Ecology Progress Series , 168 , 71 – 85 . https://doi.org/10.3354/meps168071 Search in Google Scholar

Lordan, R., Tsoupras, A., & Zabetakis, I. (2017). Phospholipids of animal and marine origin: Structure, function, and antiinflammatory properties. Molecules, 22(11), 1964. https://doi.org/10.3390/molecules22111964 Lordan R. Tsoupras A. Zabetakis I. ( 2017 ). Phospholipids of animal and marine origin: Structure, function, and antiinflammatory properties . Molecules , 22 ( 11 ), 1964 . https://doi.org/10.3390/molecules22111964 Search in Google Scholar

Marshall, C. T., Yaragina, N. A., Adlandsvik, B., & Dolgov, A. V. (2000). Reconstructing the stock-recruit relationship for Northeast Arctic cod using a bioenergetic index of reproductive potential. Canadian Journal of Fisheries and Aquatic Sciences, 57 (12), 2433–2442. https://doi.org/10.1139/f00-222 Marshall C. T. Yaragina N. A. Adlandsvik B. Dolgov A. V. ( 2000 ). Reconstructing the stock-recruit relationship for Northeast Arctic cod using a bioenergetic index of reproductive potential . Canadian Journal of Fisheries and Aquatic Sciences , 57 ( 12 ), 2433 – 2442 . https://doi.org/10.1139/f00-222 Search in Google Scholar

Marsh, J. B., & Weinstein, D. B. (1966). Simple charring method for determination of lipids. Journal of Lipid Research, 7(4), 574–576. https://doi.org/10.1016/s0022-2275(20)39274-9 Marsh J. B. Weinstein D. B. ( 1966 ). Simple charring method for determination of lipids . Journal of Lipid Research , 7 ( 4 ), 574 – 576 . https://doi.org/10.1016/s0022-2275(20)39274-9 Search in Google Scholar

Martínez-Pita, I., Sánchez-Lazo, C., Ruíz-Jarabo, I., Herrera, M., & Mancera, J. M. (2012). Biochemical composition, lipid classes, fatty acids and sexual hormones in the mussel Mytilus galloprovincialis from cultivated populations in south Spain. Aquaculture, 35(8–359), 274–283. https://doi.org/10.1016/j. aquaculture.2012.06.003 Martínez-Pita I. Sánchez-Lazo C. Ruíz-Jarabo I. Herrera M. Mancera J. M. ( 2012 ). Biochemical composition, lipid classes, fatty acids and sexual hormones in the mussel Mytilus galloprovincialis from cultivated populations in south Spain . Aquaculture , 35 ( 8–359 ), 274 – 283 . https://doi.org/10.1016/j.aquaculture.2012.06.003 Search in Google Scholar

Mika, A., Golbiowski, M., Skorkowski, E. F., & Stepnowski, P. (2012). Composition of fatty acids and sterols composition in brown shrimp Crangon crangon and herring Clupea harengus membras from the Baltic Sea. Oceanological and Hydrobiological Studies, 41(2), 57–64. https://doi. org/10.2478/s13545-012-0017-z Mika A. Golbiowski M. Skorkowski E. F. Stepnowski P. ( 2012 ). Composition of fatty acids and sterols composition in brown shrimp Crangon crangon and herring Clupea harengus membras from the Baltic Sea . Oceanological and Hydrobiological Studies , 41 ( 2 ), 57 – 64 . https://doi.org/10.2478/s13545-012-0017-z Search in Google Scholar

Moore, J. W. (1976). The proximate and fatty acid composition of some estuarine crustaceans. Estuarine and Coastal Marine Science, 4(2), 215–224. https://doi.org/10.1016/0302-3524(76)90043-8 Moore J. W. ( 1976 ). The proximate and fatty acid composition of some estuarine crustaceans . Estuarine and Coastal Marine Science , 4 ( 2 ), 215 – 224 . https://doi.org/10.1016/0302-3524(76)90043-8 Search in Google Scholar

Naeun, J., Seok-Hyun, Y., HuiTae, J., Hyo Keun, J., Yejin, K., Sanghoon, O., Jaesoon, K., Kwanwoo, K., Jae Joong, K., & Sang Heon, L. (2022). Seasonal variations in biochemical (biomolecular and amino acid) compositions and protein quality of particulate organic matter in the Southwestern East/Japan Sea. Frontiers in Marine Science, 9, 979137. https://doi.org/10.3389/fmars.2022.979137 Naeun J. Seok-Hyun Y. HuiTae J. Hyo Keun J. Yejin K. Sanghoon O. Jaesoon K. Kwanwoo K. Jae Joong K. Sang Heon L. ( 2022 ). Seasonal variations in biochemical (biomolecular and amino acid) compositions and protein quality of particulate organic matter in the Southwestern East/Japan Sea . Frontiers in Marine Science , 9 , 979137 . https://doi.org/10.3389/fmars.2022.979137 Search in Google Scholar

Nielsen, D., Hyldig, G., Nielsen, J., & Nielsen, H. H. (2005). Lipid content in herring (Clupea harengus L.) -Influence of biological factors and comparison of different methods of analyses: Solvent extraction, Fatmeter, NIR and NMR. LWT - Food Science and Technology, 38(5), 537–548. https://doi. org/10.1016/j.lwt.2004.07.010 Nielsen D. Hyldig G. Nielsen J. Nielsen H. H. ( 2005 ). Lipid content in herring (Clupea harengus L.) -Influence of biological factors and comparison of different methods of analyses: Solvent extraction, Fatmeter, NIR and NMR . LWT - Food Science and Technology , 38 ( 5 ), 537 – 548 . https://doi.org/10.1016/j.lwt.2004.07.010 Search in Google Scholar

Parrish, C. C. (1987). Separation of aquatic lipid classes by Chromarod thin-layer chromatography with measurement by Iatroscan flame ionization detection. Canadian Journal of Fisheries and Aquatic Sciences, 44(4), 722–731. https://doi.org/10.1139/f87-087 Parrish C. C. ( 1987 ). Separation of aquatic lipid classes by Chromarod thin-layer chromatography with measurement by Iatroscan flame ionization detection . Canadian Journal of Fisheries and Aquatic Sciences , 44 ( 4 ), 722 – 731 . https://doi.org/10.1139/f87-087 Search in Google Scholar

Parrish, C. C. (2013). Lipids in marine ecosystems. International Scholarly Research Notices, 604045, 16. https://doi. org/10.5402/2013/604045 Parrish C. C. ( 2013 ). Lipids in marine ecosystems . International Scholarly Research Notices , 604045 , 16 . https://doi.org/10.5402/2013/604045 Search in Google Scholar

Parrish, C. C. (2025). Production, transport, fate and effects of lipids in the marine environment. Marine Drugs, 23(2), 52. https://doi.org/10.3390/md23020052 Parrish C. C. ( 2025 ). Production, transport, fate and effects of lipids in the marine environment . Marine Drugs , 23 ( 2 ), 52 . https://doi.org/10.3390/md23020052 Search in Google Scholar

Peters, J., Renz, J., Van Beusekom, J., Boersma, M., & Hagen, W. (2006). Trophodynamics and seasonal cycle of the copepod Pseudocalanus acuspes in the Central Baltic Sea (Bornholm Basin): Evidence from lipid composition. Marine Biology, 149(6), 1417–1429. https://doi.org/10.1007/s00227-006-0290-8 Peters J. Renz J. Van Beusekom J. Boersma M. Hagen W. ( 2006 ). Trophodynamics and seasonal cycle of the copepod Pseudocalanus acuspes in the Central Baltic Sea (Bornholm Basin): Evidence from lipid composition . Marine Biology , 149 ( 6 ), 1417 – 1429 . https://doi.org/10.1007/s00227-006-0290-8 Search in Google Scholar

Prato, E., Biandolino, F., Parlapiano, I., Giandomenico, S., Denti, G., Calò, M., Spada, L., & Di Leo, A. (2019). Proximate, fatty acids and metals in edible marine bivalves from Italian market: Beneficial and risk for consumers health. Science of the Total Environment, 648(6), 153–163. https://doi. org/10.1016/j.scitotenv.2018.07.382 Prato E. Biandolino F. Parlapiano I. Giandomenico S. Denti G. Calò M. Spada L. Di Leo A. ( 2019 ). Proximate, fatty acids and metals in edible marine bivalves from Italian market: Beneficial and risk for consumers health . Science of the Total Environment , 648 ( 6 ), 153 – 163 . https://doi.org/10.1016/j.scitotenv.2018.07.382 Search in Google Scholar

Pusceddu, A., Dell’Anno, A., Fabiano, M., & Danovaro, R. (2009). Quantity and bioavailability of sediment organic matter as signatures of benthic trophic status. Marine Ecology Progress Series, 5, 41–52. https://doi.org/10.3354/meps07735 Pusceddu A. Dell’Anno A. Fabiano M. Danovaro R. ( 2009 ). Quantity and bioavailability of sediment organic matter as signatures of benthic trophic status . Marine Ecology Progress Series , 5 , 41 – 52 . https://doi.org/10.3354/meps07735 Search in Google Scholar

Reinhardt, S. B., & Van Vleet, E. S. (1986). Lipid composition of twenty-two species of Antarctic midwater zooplankton and fish. Marine Biology, 91, 149–159. https://doi. org/10.1007/BF00569431 Reinhardt S. B. Van Vleet E. S. ( 1986 ). Lipid composition of twenty-two species of Antarctic midwater zooplankton and fish . Marine Biology , 91 , 149 – 159 . https://doi.org/10.1007/BF00569431 Search in Google Scholar

Reisenbichler, K. R., & Bailey, T. G. (1991). Microextraction of total lipid from mesopelagic animals. Deep Sea Research Part A. Oceanographic Research Papers, 38(10), 1331–1339. https://doi.org/10.1016/0198-0149(91)90030-J Reisenbichler K. R. Bailey T. G. ( 1991 ). Microextraction of total lipid from mesopelagic animals . Deep Sea Research Part A. Oceanographic Research Papers , 38 ( 10 ), 1331 – 1339 . https://doi.org/10.1016/0198-0149(91)90030-J Search in Google Scholar

Ridgway, N., & McLeod, R. (2016). Biochemistry of lipids, lipoproteins and membranes (6th ed.). Elsevier. Ridgway N. McLeod R. ( 2016 ). Biochemistry of lipids, lipoproteins and membranes ( 6th ed.). Elsevier . Search in Google Scholar

Skoog, D. A., West, D. M., Holler, F. J., & Crouch, S. R. (2013). Random errors in chemical analysis. In D. A. Skoog, D. M. West, F. J. Holler, & S. R. Crouch (Eds.), Fundamental of analytical chemistry (pp. 93–122). Cengage Learning. Skoog D. A. West D. M. Holler F. J. Crouch S. R. ( 2013 ). Random errors in chemical analysis . In Skoog D. A. West D. M. Holler F. J. Crouch S. R. (Eds.), Fundamental of analytical chemistry (pp. 93 – 122 ). Cengage Learning . Search in Google Scholar

Sokołowski, A., Wołowicz, M., & Hummel, H. (2003). Free amino acids in the clam Macoma balthica L. (Bivalvia, Mollusca) from brackish waters of the southern Baltic Sea. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 134(3), 579–592. https://doi.org/10.1016/S1095-6433(02)00360-4 Sokołowski A. Wołowicz M. Hummel H. ( 2003 ). Free amino acids in the clam Macoma balthica L. (Bivalvia, Mollusca) from brackish waters of the southern Baltic Sea . Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology , 134 ( 3 ), 579 – 592 . https://doi.org/10.1016/S1095-6433(02)00360-4 Search in Google Scholar

Sokołowski, A., Ziółkowska, M., Balazy, P., Plichta, I., Kukliński, P., & Mudrak-Cegiołka, S. (2017). Recruitment pattern of benthic fauna on artificial substrates in brackish low-diversity system (the Baltic Sea). Hydrobiologia, 784(1), 125–141. https://doi.org/10.1007/s10750-016-2862-z Sokołowski A. Ziółkowska M. Balazy P. Plichta I. Kukliński P. Mudrak-Cegiołka S. ( 2017 ). Recruitment pattern of benthic fauna on artificial substrates in brackish low-diversity system (the Baltic Sea) . Hydrobiologia , 784 ( 1 ), 125 – 141 . https://doi.org/10.1007/s10750-016-2862-z Search in Google Scholar

Stefanov, K. L., Christie, W. W., Brechany, E. Y., Popov, S. S., & Andreev, S. N. (1992). Lipid composition of the red and green forms of Actina equinia from the black sea. Comparative Biochemistry and Physiology. Part B, Biochemistry, 103(3), 687–690. https://doi.org/10.1016/0305-0491(92)90391-4 Stefanov K. L. Christie W. W. Brechany E. Y. Popov S. S. Andreev S. N. ( 1992 ). Lipid composition of the red and green forms of Actina equinia from the black sea . Comparative Biochemistry and Physiology. Part B, Biochemistry , 103 ( 3 ), 687 – 690 . https://doi.org/10.1016/0305-0491(92)90391-4 Search in Google Scholar

Stelzer, P. S., Mazzuco, A. C. A., Gomes, L. E., Martins, J., Netto, S., & Bernardino, A. F. (2021). Taxonomic and functional diversity of benthic macrofauna associated with rhodolith beds in SE Brazil. PeerJ, 9, e11903. https://doi.org/10.7717/peerj.11903 Stelzer P. S. Mazzuco A. C. A. Gomes L. E. Martins J. Netto S. Bernardino A. F. ( 2021 ). Taxonomic and functional diversity of benthic macrofauna associated with rhodolith beds in SE Brazil . PeerJ , 9 , e11903 . https://doi.org/10.7717/peerj.11903 Search in Google Scholar

Sukhotin, A. A., Lajus, D. L., & Lesin, P. A. (2003). Influence of age and size on pumping activity and stress resistance in the marine bivalve Mytilus edulis L. The Journal of Experimental Marine Biology and Ecology, 284(1-2), 129–144. https://doi. org/10.1016/S0022-0981(02)00497-5 Sukhotin A. A. Lajus D. L. Lesin P. A. ( 2003 ). Influence of age and size on pumping activity and stress resistance in the marine bivalve Mytilus edulis L . The Journal of Experimental Marine Biology and Ecology , 284 ( 1-2 ), 129 – 144 . https://doi.org/10.1016/S0022-0981(02)00497-5 Search in Google Scholar

Szaniawska, A. (1983). Seasonal changes in energy content of Crangon crangon L. (Crustacea, Decapoda). Polish Archives of Hydrobiology, 30(1), 45–56. Szaniawska A. ( 1983 ). Seasonal changes in energy content of Crangon crangon L. (Crustacea, Decapoda) . Polish Archives of Hydrobiology , 30 ( 1 ), 45 – 56 . Search in Google Scholar

Taverniers, I., De Loose, M., & Van Bockstaele, E. (2004). Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance. Trends in Analytical Chemistry, 23(8), 535–552. https://doi.org/10.1016/j. trac.2004.04.001 Taverniers I. De Loose M. Van Bockstaele E. ( 2004 ). Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance . Trends in Analytical Chemistry , 23 ( 8 ), 535 – 552 . https://doi.org/10.1016/j.trac.2004.04.001 Search in Google Scholar

Taylor, J. (2022). An introduction to error analysis: The study of uncertainties in physical measurements (3rd. ed.). University Science Books. Taylor J. ( 2022 ). An introduction to error analysis: The study of uncertainties in physical measurements ( 3rd . ed.). University Science Books . Search in Google Scholar

Wang, H., Seekamp, I., Malzahn, A., Hagemann, A., Carvajal, A. K., Slizyte, R., Standal, I. B., Handå, A., & Reitan, K. I. (2019). Growth and nutritional composition of the polychaete Hediste diversicolor (OF Müller, 1776) cultivated on waste from landbased salmon SMOLT aquaculture. Aquaculture, 502(15), 232–241. https://doi.org/10.1016/j.aquaculture.2018.12.047 Wang H. Seekamp I. Malzahn A. Hagemann A. Carvajal A. K. Slizyte R. Standal I. B. Handå A. Reitan K. I. ( 2019 ). Growth and nutritional composition of the polychaete Hediste diversicolor (OF Müller, 1776) cultivated on waste from landbased salmon SMOLT aquaculture . Aquaculture , 502 ( 15 ), 232 – 241 . https://doi.org/10.1016/j.aquaculture.2018.12.047 Search in Google Scholar

Webster, G. K., Kotts, L., & Maloney, T. D. (2005). Considerations when implementing automated methods into GXP laboratories. Journal of Laboratory Automation, 10(3), 182–191. https://doi.org/10.1016/j.jala.2005.03.003 Webster G. K. Kotts L. Maloney T. D. ( 2005 ). Considerations when implementing automated methods into GXP laboratories . Journal of Laboratory Automation , 10 ( 3 ), 182 – 191 . https://doi.org/10.1016/j.jala.2005.03.003 Search in Google Scholar

Wiklund, A. K. E., Dahlgren, K., Sundelin, B., & Andersson, A. (2009). Effects of warming and shifts of pelagic food web structure on benthic productivity in a coastal marine system. Marine Ecology Progress Series, 396, 13–25. https://doi.org/10.3354/meps08290 Wiklund A. K. E. Dahlgren K. Sundelin B. Andersson A. ( 2009 ). Effects of warming and shifts of pelagic food web structure on benthic productivity in a coastal marine system . Marine Ecology Progress Series , 396 , 13 – 25 . https://doi.org/10.3354/meps08290 Search in Google Scholar

Wołowicz, M., Sokołowski, A., Bawazir, A. S., & Lasota, R. (2006). Effect of eutrophication on the distribution and ecophysiology of the mussel Mytilus trossulus (Bivalvia) in southern Baltic Sea (the Gulf of Gdańsk). Limnology and Oceanography, 51(1, part 2), 580–590. https://doi. org/10.4319/lo.2006.51.1_part_2.0580 Wołowicz M. Sokołowski A. Bawazir A. S. Lasota R. ( 2006 ). Effect of eutrophication on the distribution and ecophysiology of the mussel Mytilus trossulus (Bivalvia) in southern Baltic Sea (the Gulf of Gdańsk) . Limnology and Oceanography , 51 ( 1, part 2 ), 580 – 590 . https://doi.org/10.4319/lo.2006.51.1_part_2.0580 Search in Google Scholar