Development of human cell biosensor system for genotoxicity detection based on DNA damage-induced gene expression

Elespuru RK, Agarwal R, Atrakchi AH, Bigger CAH, Heflich RH, Jagannath DR, et al. Current and Future Application of Genetic Toxicity Assays: The Role and Value of In Vitro Mammalian Assays. Toxicol Sci 2009; 109: 172-9.10.1093/toxsci/kfp067Search in Google Scholar

Sutton MD, Smith BT, Godoy VG, Walker GC. The SOS response: Recent insights into umuDC-dependent mutagenesis and DNA damage tolerance. Ann Rev Genet 2000; 34: 479-97.10.1146/annurev.genet.34.1.479Search in Google Scholar

Putnam CD, Jaehnig EJ, Kolodner RD. Perspectives on the DNA damage and replication checkpoint responses in Saccharomyces cerevisiae. DNA Repair 2009; 8: 974-82.10.1016/j.dnarep.2009.04.021Search in Google Scholar

Holbrook NJ, Fornace AJ. Response to adversity - molecular control of gene activation following genotoxic stress. New Biologist 1991; 3: 825-33.Search in Google Scholar

Quillardet P, Huisman O, Dari R, Hofnung M. SOS chromotest, a direct assay of induction of an sos function in escherichia-coli k-12 to measure genotoxicity. P Natl Acad Sci USA 1982; 79: 5971-5.10.1073/pnas.79.19.5971Search in Google Scholar

Oda Y, Nakamura S, Oki I, Kató T, Shinagawa H. Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. Mutat Res 1985; 147: 219-29.10.1016/0165-1161(85)90062-7Search in Google Scholar

Walmsley RM, Billinton N, Walsh L, Barker MG, Knight AW, Cahill PA. A yeast RAD54-GFP genotoxicity assay, is effective in identifying direct acting mutagens in addition to clastogens not detected by bacterial tests. Toxicol Sci 2003; 72: 1106.Search in Google Scholar

Liu X, Kramer JA, Swaffield JC, Hu Y, Chai G, Wilson AGE. Development of a highthroughput yeast-based assay for detection of metabolically activated genotoxins. Mutat Res-Gen Tox En 2008; 653: 63-9.10.1016/j.mrgentox.2008.03.00618485802Search in Google Scholar

Zhou B-BS, Elledge SJ. The DNA damage response: putting checkpoints in perspective. Nature 2000; 408: 433-9.10.1038/3504400511100718Search in Google Scholar

Sionov RV, Haupt Y. The cellular response to p53: the decision between life and death. Oncogene 1999; 18: 6145-57.10.1038/sj.onc.120313010557106Search in Google Scholar

Waldman T, Kinzler KW, Vogelstein B. P21 is necessary for the P53-mediated G1 arrest in human cancer cells. Cancer Res 1995; 55: 5187-90.Search in Google Scholar

Vogelstein B, Lane D, Levine AJ. Surfing the p53 network. Nature 2000; 408: 307-10.10.1038/3504267511099028Search in Google Scholar

Moldovan G-L, Pfander B, Jentsch S. PCNA, the maestro of the replication fork. Cell 2007; 129: 665-79.10.1016/j.cell.2007.05.00317512402Search in Google Scholar

Park SY, Lee SM, Ye SK, Yoon SH, Chung MH, Choi J. Benzo[a]pyrene-induced DNA damage and p53 modulation in human hepatoma HepG2 cells for the identification of potential biomarkers for PAH monitoring and risk assessment. Toxicol Lett 2006; 167: 27-33.10.1016/j.toxlet.2006.08.01117029827Search in Google Scholar

Zegura B, Zajc I, Lah TT, Filipic M. Patterns of microcystin-LR induced alteration of the expression of genes involved in response to DNA damage and apoptosis. Toxicon 2008; 51: 615-23.10.1016/j.toxicon.2007.11.00918191168Search in Google Scholar

Hreljac I, Zajc I, Lah T, Filipic M. Effects of model organophosphorous pesticides on DNA damage and proliferation of HepG2 cells. Environ Mol Mutagen 2008; 49: 360-7.10.1002/em.2039218418871Search in Google Scholar

Ellinger-Ziegelbauer H, Stuart B, Wahle B, Bomann W, Ahr HJ. Comparison of the expression profiles induced by genotoxic and nongenotoxic carcinogens in rat liver. Mutat Res-Gen Tox En 2005; 575: 61-84.10.1016/j.mrfmmm.2005.02.00415890375Search in Google Scholar

Knasmuller S, Mersch-Sundermann V, Kevekordes S, Darroudi F, Huber WW, Hoelzl C, et al.. Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants; current state of knowledge. Toxicology 2004; 198: 315-28.10.1016/j.tox.2004.02.00815138058Search in Google Scholar

Bressac B, Galvin KM, Liang TJ, Isselbacher KJ, Wands JR, Ozturk M. Abnormal structure and expression of p53 gene in human hepatocellular-carcinoma. P Natl Acad Sci USA 1990; 87: 1973-7.10.1073/pnas.87.5.1973536072155427Search in Google Scholar

Mesojednik S, Kamensek U, Cemazar M. Evaluation of shRNA-mediated gene silencing by electroporation in LPB fibrosarcoma cells. Radiol Oncol 2008; 42: 82-92.10.2478/v10019-008-0007-3Search in Google Scholar

Todd MD, Lee MJ, Williams JL, Nalezny JM, Gee P, Benjamin MB, et al. The cat-tox (l) assay - a sensitive and specific measure of stress-induced transcription in transformed human liver-cells. Fund Appl Toxicol 1995; 28: 118-28.10.1006/faat.1995.11538566476Search in Google Scholar

Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature 2000; 404: 42-9.10.1038/3500350610716435Search in Google Scholar

Guittet O, Hakansson P, Voevodskaya N, Fridd S, Graslund A, Arakawa H, et al. Mammalian p53R2 protein forms an active ribonucleotide reductase in vitro with the R1 protein, which is expressed both in resting cells in response to DNA damage and in proliferating cells. J Biol Chem 2001; 276: 40647-51.10.1074/jbc.M10608820011517226Search in Google Scholar

Ohno K, Tanaka-Azuma Y, Yoneda Y, Yamada T. Genotoxicity test system based on p53R2 gene expression in human cells: Examination with 80 chemicals. Mutat Res-Gen Tox En 2005; 588: 47-57.10.1016/j.mrgentox.2005.09.002Search in Google Scholar

Ohno K, Ishihata K, Tanaka-Azuma Y, Yamada T. A genotoxicity test system based on p53R2 gene expression in human cells: Assessment of its reactivity to various classes of genotoxic chemicals. Mutat Res-Gen Tox En 2008; 656: 27-35.10.1016/j.mrgentox.2008.07.002Search in Google Scholar

Siafakas RA, Richardson DR. Growth arrest and DNA damage-45 alpha (GADD45α). Int J BiochemCell B 2009; 41: 986-9.Search in Google Scholar

Hastwell PW, Chai LL, Roberts KJ, Webster TW, Harvey JS, Rees RW, et al. High-specificity and high-sensitivity genotoxicity assessment in a human cell line: Validation of the GreenScreen HC GADD45a-GFP genotoxicity assay. Mutat Res-Gen Tox En 2006; 607: 160-75.10.1016/j.mrgentox.2006.04.011Search in Google Scholar

Birrell L, Cahill P, Hughes C, Tate M, Walmsley RM. GADD45a-GFP GreenScreen HC assay results for the ECVAM recommended lists of genotoxic and non-genotoxic chemicals for assessment of new genotoxicity tests. Mutat Res-Gen Tox En 2010; 695: 87-95.10.1016/j.mrgentox.2009.12.008Search in Google Scholar

Zhang R, Niu YJ, Do HR, Cao XW, Shi D, Hao QL, et al. A stable and sensitive testing system for potential carcinogens based on DNA damage-induced gene expression in human HepG2 cell. Toxicol In Vitro 2009; 23: 158-65.10.1016/j.tiv.2008.10.006Search in Google Scholar

Yang TT, Cheng LZ, Kain SR. Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein. Nucleic Acids Res 1996; 24: 4592-3.10.1093/nar/24.22.4592Search in Google Scholar

Cormack BP, Valdivia RH, Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996; 173: 33-8.10.1016/0378-1119(95)00685-0Search in Google Scholar

Lawley PD. Mutagens as carcinogens - development of current concepts. Mutat Res 1989; 213: 3-25.10.1016/0027-5107(89)90028-6Search in Google Scholar

Beranek DT. Distribution of methyl and ethyl adducts following alkylation with monofunctional alkylating-agents. Mutat Res 1990; 231: 11-30.10.1016/0027-5107(90)90173-2Search in Google Scholar

Jaiswal AS, Narayan S. S(N)2 DNA-alkylating agent-induced phosphorylation of p53 and activation of p21 gene expression. Mutat Res-Fund Mol M 2002; 500: 17-30.10.1016/S0027-5107(01)00296-2Search in Google Scholar

Perlow RA, Kolbanovskii A, Hingerty BE, Geacintov NE, Broyde S, Scicchitano DA. DNA adducts from a tumorigenic metabolite of benzo[a]pyrene block human RNA polymerase II elongation in a sequence- and stereochemistry-dependent manner. J Mol Biol 2002; 321: 29-47.10.1016/S0022-2836(02)00593-4Search in Google Scholar

Wang A, Gu J, Judson-Kremer K, Powell KL, Mistry H, Simhambhatla P, et al. Response of human mammary epithelial cells to DNA damage induced by BPDE: involvement of novel regulatory pathways. Carcinogenesis 2003; 24: 225-34.10.1093/carcin/24.2.22512584171Search in Google Scholar

Sadikovic B, Rodenhiser DI. Benzopyrene exposure disrupts DNA methylation and growth dynamics in breast cancer cells. Toxicol Appl Pharm 2006; 216: 458-68.10.1016/j.taap.2006.06.01216926039Search in Google Scholar

Jagger C, Tate M, Cahill PA, Hughes C, Knight AW, Billinton N, et al. Assessment of the genotoxicity of S9-generated metabolites using the GreenScreen HC GADD45a-GFP assay. Mutagenesis 2009; 24: 35-50.10.1093/mutage/gen05018787182Search in Google Scholar

Corda Y, Job C, Anin MF, Leng M, Job D. Spectrum of DNA platinum adduct recognition by prokaryotic and eukaryotic DNA-dependent RNA-polymerases. Biochemistry 1993; 32: 8582-8.10.1021/bi00084a0277689335Search in Google Scholar

Pabla N, Huang S, Mi QS, Daniel R, Dong Z. ATR-Chk2 signaling in p53 activation and DNA damage response during cisplatin-induced apoptosis. J Biol Chem 2008; 283: 6572-83.10.1074/jbc.M70756820018162465Search in Google Scholar

Marini F, Nardo T, Giannattasio M, Minuzzo M, Stefanini M, Plevani P, et al.. DNA nucleotide excision repair-dependent signaling to checkpoint activation. P Natl Acad Sci USA 2006; 103: 17325-30.10.1073/pnas.0605446103185992917088560Search in Google Scholar

Owellen RJ, Hartke CA, Dickerson RM, Hains FO. Inhibition of tubulin-microtubule polymerization by drugs of vinca alkaloid class. Cancer Res 1976; 36: 1499-502.Search in Google Scholar

Tishler RB, Lamppu DM, Park S, Price BD. Microtubule-active drugs taxol, vinblastine, and nocodazole increase the levels of transcriptionally active P53. Cancer Res 1995; 55: 6021-5.Search in Google Scholar