Forest tree breeding faces challenges due to long generation times and high costs. To address these issues, the concept of “breeding without breeding” (BwB) offers the opportunity to accelerate breeding cycles by using naturally generated half- sib and full-sib families. An isolated population of trees may provide an ideal environment to identify full-sib families for genetic mapping without the need for controlled pollination. In this study, paternity analysis based on genotyping of 16 SSR markers in 1232 seedlings identified three large full-sib families of