Analysis of magnetic resonance contrast agent entrapment following reversible electroporation in vitro
Kategoria artykułu: Research Article
Data publikacji: 15 wrz 2024
Zakres stron: 406 - 415
Otrzymano: 31 lip 2024
Przyjęty: 09 sie 2024
DOI: https://doi.org/10.2478/raon-2024-0047
Słowa kluczowe
© 2024 Marko Strucic et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
Administering gadolinium-based contrast agent before electroporation allows the contrast agent to enter the cells and enables MRI assessment of reversibly electroporated regions. The aim of this study was evaluation of contrast agent entrapment in Chinese hamster ovary (CHO) cells and comparison of these results with those determined by standard
Materials and methods
Cell membrane permeabilization and cell membrane integrity experiments were performed using YO-PRO-1 dye and propidium iodide, respectively. Cell survival experiments were performed by assessing metabolic activity of cells using MTS assay. The entrapment of gadolinium-based contrast agent gadobutrol inside the cells was evaluated using T1 relaxometry of cell suspensions 25 min and 24 h after electroporation and confirmed by inductively coupled plasma mass spectrometry.
Results
Contrast agent was detected 25 min and 24 h after the delivery of electric pulses in cells that were reversibly electroporated. In addition, contrast agent was present in irreversibly electroporated cells 25 min after the delivery of electric pulses but was no longer detected in irreversibly electroporated cells after 24 h. Inductively coupled plasma mass spectrometry showed a proportional decrease in gadolinium content per cell with shortening of T1 relaxation time (
Conclusions
Our results demonstrate that the contrast agent is entrapped in cells exposed to reversible electroporation but exits from cells exposed to irreversible electroporation within 24 h, thus confirming the hypothesis on which detection experiments