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The diagnostic value of selected immune parameters in peripheral blood of dogs with malignant mammary tumours – a preliminary study

Urszula Lisiecka
Urszula Lisiecka
, 
Piotr Brodzki
Piotr Brodzki
, 
Anna Śmiech
Anna Śmiech
, 
Katarzyna Michalak
Katarzyna Michalak
, 
Stanisław Winiarczyk
Stanisław Winiarczyk
, 
Beata Żylińska
Beata Żylińska
 oraz 
Łukasz Adaszek
Łukasz Adaszek
   | 20 cze 2024
Journal of Veterinary Research's Cover Image
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Data publikacji: 20 cze 2024
Zakres stron: 271 - 278
Otrzymano: 28 mar 2024
Przyjęty: 13 cze 2024
DOI: https://doi.org/10.2478/jvetres-2024-0035
Słowa kluczowe
© 2024 Urszula Lisiecka et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Frequencies of CD3+ T lymphocytes (A) and CD21+ B lymphocytes (B), and the CD3+/CD21+ lymphocyte ratio (C) in blood from canine mammary tumour patients and healthy controls. Mean and median percentages of cells in the control group (H), in the group without metastases (WM), and in the group with metastases (M). Boxes cover the 25th–75th percentiles and the median is shown as a line across the box * – statistically significant difference (P-value < 0.05) between group WM and group M; X – statistically significant difference (P-value < 0.05) between groups WM or M and group H
Frequencies of CD3+ T lymphocytes (A) and CD21+ B lymphocytes (B), and the CD3+/CD21+ lymphocyte ratio (C) in blood from canine mammary tumour patients and healthy controls. Mean and median percentages of cells in the control group (H), in the group without metastases (WM), and in the group with metastases (M). Boxes cover the 25th–75th percentiles and the median is shown as a line across the box * – statistically significant difference (P-value < 0.05) between group WM and group M; X – statistically significant difference (P-value < 0.05) between groups WM or M and group H

Fig. 2.

Frequencies of CD4+ T helper lymphocytes (A) and CD8+ cytotoxic T lymphocytes (B), and the CD4+/CD8+ lymphocyte ratio (C) in blood from canine mammary tumour patients and healthy controls. Mean and median percentages of cells in the control group (H), in the group without metastases (WM), and in the group with metastases (M). Boxes cover the 25th–75th percentiles and the median is shown as a line across the box * – statistically significant difference (P-value < 0.05) between group WM and group M; X – statistically significant difference (P-value < 0.05) between groups WM or M and group H
Frequencies of CD4+ T helper lymphocytes (A) and CD8+ cytotoxic T lymphocytes (B), and the CD4+/CD8+ lymphocyte ratio (C) in blood from canine mammary tumour patients and healthy controls. Mean and median percentages of cells in the control group (H), in the group without metastases (WM), and in the group with metastases (M). Boxes cover the 25th–75th percentiles and the median is shown as a line across the box * – statistically significant difference (P-value < 0.05) between group WM and group M; X – statistically significant difference (P-value < 0.05) between groups WM or M and group H

Descriptive statistics of variables (full sample set n = 30)

Variable Health status Mean Median SD SE Minimum Maximum ANOVA P-value Tukey pairs Tukey P-values
% CD3+ without metastasis 66.6 69.3 8.9 2.8 52.7 80.6 0.0003 H vs MWM vs M 0.0010.0011
metastasis 46.1 46.4 14.7 4.7 22.3 68.9
healthy 66.7 67.1 9.3 2.9 52.5 85.1
% CD21+ without metastasis 8.5 8.6 2.5 0.8 5.2 11.9 0.0018 H vs M WM vs M 0.01390.0021
metastasis 20.1 17.9 10.4 3.3 8.4 38.2
healthy 10.9 10.9 4.9 1.6 4.6 21.4
% CD4+ without metastasis 35.5 34.9 9.5 3.0 22.6 50.4 0.0002 H vs WMWM vs M 0.00020.0061
metastasis 23.2 25.9 8.3 2.6 10.3 34.6
healthy 40.4 42.3 6.2 2.0 31.4 52.0
% CD8+ without metastasis 20.7 19.4 8.0 2.5 9.5 35.4 No statistically significant difference
metastasis 19.3 20.3 9.0 2.8 4.5 36.4
healthy 18.4 18.3 2.3 0.7 15.1 21.9
CD3+/CD21+ratio without metastasis 8.5 8.6 2.8 0.9 4.4 13.3 0.0028 H vs MWM vs M 0.01250.0041
metastasis 3.0 2.7 2.1 0.7 0.6 8.1
healthy 7.8 6.5 4.9 1.5 2.8 18.0
CD4+/CD8+ ratio without metastasis 1.9 1.9 0.9 0.3 0.8 3.3 0.0348 H vs M 0.03291
metastasis 1.4 1.3 0.5 0.2 0.6 2.3
healthy 1.2 2.2 0.7 0.2 1.4 3.4

Characteristics of canine patients enrolled in the study

Characteristics Number of patients with different stages of tumour
Without metastases (N0M0) With metastases (N1, N0M1 or N1M1)
G1 complex carcinoma 2 0
G2 complex carcinoma 2 2
G1 tubular carcinoma 3 2
G2 tubular carcinoma 2 3
osteosarcoma 1 3
eISSN:
2450-8608
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Molecular Biology, other, Microbiology and Virology, Medicine, Veterinary Medicine
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