Matrine and icariin can inhibit bovine viral diarrhoea virus replication by promoting type I interferon response in vitro
Dongjie Cai
Profil Orcid, Zifan Shen
Profil Orcid, Bin Tian
Profil Orcid, Jie Chen
Profil Orcid, Yilin Zhang
- College of Veterinary Medicine, Sichuan Agricultural UniversityChengdu, China
- Laboratory of Animal Disease Prevention and Control Center, Agriculture and Rural Affairs Bureau of Luoping CountyQujing, China
Profil Orcid, Liuhong Shen
Profil Orcid, Ya Wang
Profil Orcid, Xiaoping Ma
Profil Orcid oraz Zhicai Zuo
Profil Orcid 
Data publikacji: 23 mar 2024
Zakres stron: 35 - 44
Otrzymano: 19 sie 2023
Przyjęty: 01 mar 2024
DOI: https://doi.org/10.2478/jvetres-2024-0013
Słowa kluczowe
© 2024 Dongjie Cai et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Bovine viral diarrhoea virus (BVDV) can cause diarrhoea (BVD) in an animal herd, leading to heavy economic losses. There are limited drugs available for treating and controlling BVD. This research aims to investigate the antiviral and immunoregulatory effects of two traditional Chinese herb extracts against BVDV infection. The extracts are matrine and icariin, which have been proved to have immunostimulant and antiviral effects.
Material and Methods
A cell counting kit-8 assay was used to analyse the toxicity of matrine and icariin to Madin–Darby bovine kidney (MDBK) cells. The model of MDBK cells infected with BVDV was utilised to uncover the antiviral mechanism of matrine and icariin, which along with their immunoregulatory ability was evaluated by quantitative reverse-transcription PCR and ELISA.
Results
The results showed that matrine and icariin can significantly inhibit the gene expression level of the BVDV 5′ untranslated region through various pathways. Both matrine and icariin can statistically upregulate the gene expression level of interferon alpha, interferon beta (IFN-β), toll-like receptor 3, retinoic acid–inducible gene I and interferon regulatory factor 3, and raise the concentration of IFN-β after BVDV infection.
Conclusion
This study proves that both matrine and icariin have inhibitory effects on BVDV replication by activating IFN production and the IFN signalling pathway. The finding is promising and should open up the possibility of larger-scale