The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon
Bartłomiej Szymczak
Profil Orcid, Andrzej Junkuszew
Profil Orcid, Krzysztof Patkowski
Profil Orcid, Tomasz Szponder
Profil Orcid, Dominika Nguyen Ngoc
Profil Orcid, Beata Drzewiecka
Profil Orcid, Aleksandra Sobczyńska-Rak
Profil Orcid oraz Joanna Wessely-Szponder
Profil Orcid 
Data publikacji: 23 mar 2024
Zakres stron: 167 - 174
Otrzymano: 28 lip 2023
Przyjęty: 15 sty 2024
DOI: https://doi.org/10.2478/jvetres-2024-0003
Słowa kluczowe
© 2024 Bartłomiej Szymczak et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Macrophages are crucial immune cells that play a role in tissue repair and can exhibit pro- or anti-inflammatory behaviour based on environmental stimulation. Their functional phenotype can be affected by platelet-derived products as determined by those products’ composition. When the inflammatory response caused by implantation is excessive, it can lead to rejection of the implant. Therefore, a thorough evaluation of implant haemocompatibility is necessary to minimise undesirable consequences.
Material and Methods
In an
Results
Platelet-poor plasma (PPP) and pure platelet-rich plasma (P-PRP) stimulation increased arginase-1 activity, while leukocyte-rich PRP stimulation produced a mixed response involving higher O2− (6.49 ± 2.43 nM
Conclusion
Using PPP and P-PRP stimulation in post-implantation procedures may contribute to the polarisation of macrophages towards the M2-like pro-resolving phenotype, thereby accelerating wound healing. This would also prevent implant degradation due to an excessive inflammatory process.