Protein sequence features of H1N1 swine influenza A viruses detected on commercial swine farms in Serbia
, , , , , oraz | 16 cze 2023
O artykule
Data publikacji: 16 cze 2023
Zakres stron: 147 - 154
Otrzymano: 07 lut 2023
Przyjęty: 02 cze 2023
DOI: https://doi.org/10.2478/jvetres-2023-0034
Słowa kluczowe
© 2023 J.M. Zorić et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
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Analysed amino acid mutations within proteins of Serbian swine influenza A virus strains and their significance
| Protein | Analysed mutation/amino acid position | Significance | References |
|---|---|---|---|
| PB2 | E627K | • Polymerase activity in mammalian cells | 5, 15, 39 |
| D701N | • Polymerase activity in mammalian cells | ||
| G590S/Q591R | • Polymerase activity in mammalian cells | ||
| T271A | • Enhanced polymerase activity and virus growth of H1N1pdm09 viruses in mammalian hosts | ||
| D9N | • B-interferon inhibition in mitochondria | ||
| R251K | • Virulence of H1avN1 viruses | ||
| PA | I38T | • Antiviral drug (baloxavir marboxil) resistance | 35 |
| HA | Sa (128, 129, 158, 160, 162–167) | • Antigenic drift | 7, 42 |
| Sb (156, 159 187–198) | |||
| Ca1 (169–173 206–208 238–240) | |||
| Ca2 (140–145 224–225) | |||
| Cb (74–79, 83,122) | |||
| NP | N313, V100I, 350K/T, 371V/M, 444V/I, 456L/V | • Lineage characteristics | 34, 45 |
| NA | E119G/V, Q136K/R, K150T, D199G, I223K/R/V, S247N, H275Y, N295S | • Resistance to neuraminidase inhibitors (oseltamivir, zanamivir, peramivir) | 6, 16, 19 |
| M2 | L26F/I, V27A/T, A30T/V, S31N, G34E, L38F | • Resistance to adamantane M2 ion channel inhibitors (amantadine, rimantadine) | 11 |
| NS1 | D125G | • Adaptation to new host species, synthesis of NS3 protein | 40 |
| K108R, D125E, D189G | • Antiviral activity of host cells | 12 |
Results of the comparison of haemagglutinin (HA) antigenic determinants between Serbian swine influenza A virus strains and vaccine strains
| SRB/sw/2017/2pdm | SRB/sw/2016/3pdm | |||
| Vaccine strain | H1 HA antigenic site | Amino acid substitution | ||
| A/Jena/VI5258/2009 (H1N1pdm09) | Sa | G158V, L164I | N129D, G158V, L164I | |
| Sb | - | - | ||
| Ca1 | - | S206T | ||
| Ca2 | S146G | A144E | ||
| Cb | - | - | ||
| SRB/sw/2017/1av | SRB/sw/2016/5av | SRB/sw/2017/7av | ||
| H1 HA antigenic site | Amino acid substitution | |||
| A/swine/Haselünne/2617/2003 (H1avN1) | Sa | K166I | N129E, S165N | |
| Sb | N159G, Q192L | |||
| Ca1 | T169A | Q239K | ||
| Ca2 | H141Y, G225E | A144S, G225E | A144T, G225E | |
| Cb | L78F | |||
Genotype composition of Serbian swine influenza A viruses
| Virus (subtype) | Abbreviation | Genotype | |||||||
|---|---|---|---|---|---|---|---|---|---|
| PB2 | PB1 | PA | HA | NP | NA | M | NS | ||
| A/swine/Serbia/1/2017(H1N1) | SRB/sw/2017/1 | ||||||||
| A/swine/Serbia/2/2017(H1N1) | SRB/sw/2017/2 | ||||||||
| A/swine/Serbia/3/2016(H1N1) | SRB/sw/2016/3 | ||||||||
| A/swine/Serbia/5/2016(H1N1) | SRB/sw/2016/5 | ||||||||
| A/swine/Serbia/7/2017(H1N1) | SRB/sw/2017/7 | ||||||||