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Analysis of the effect of polymorphisms within the CATHL7 gene on dairy performance parameters

Sonia Hiller
Sonia Hiller
, 
Inga Kowalewska
Inga Kowalewska
, 
Ewa Czerniawska-Piątkowska
Ewa Czerniawska-Piątkowska
 oraz 
Dorota Banaszewska
Dorota Banaszewska
   | 28 mar 2023
Journal of Veterinary Research's Cover Image
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Data publikacji: 28 mar 2023
Zakres stron: 123 - 129
Otrzymano: 05 sty 2023
Przyjęty: 10 mar 2023
DOI: https://doi.org/10.2478/jvetres-2023-0018
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© 2023 S. Hiller et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Agarose gel electrophoresis (3%) of PCR-restriction fragment length polymorphism result for CATHL7/HhaI polymorphism. From left: pUC19/MspI DNA ladder (501/489, 404, 331, 242, 190, 147, 111/110, 67 and 34 base pairs (bp)), CC genotype (324 and 23 bp), CG genotype (347, 324 and 23 bp), and GG genotype (347 bp); the 23 bp fragment is not visible because of its size and diffusion in gel
Agarose gel electrophoresis (3%) of PCR-restriction fragment length polymorphism result for CATHL7/HhaI polymorphism. From left: pUC19/MspI DNA ladder (501/489, 404, 331, 242, 190, 147, 111/110, 67 and 34 base pairs (bp)), CC genotype (324 and 23 bp), CG genotype (347, 324 and 23 bp), and GG genotype (347 bp); the 23 bp fragment is not visible because of its size and diffusion in gel

Fig. 2

Agarose gel electrophoresis (3%) of PCR-restriction fragment length polymorphism result for CATHL7/HinfI polymorphism. From left: pUC19/MspI DNA ladder (501/489, 404, 331, 242, 190, 147, 111/110, 67 and 34 base pairs (bp)), CC genotype (161, 81, 58, 34 and 14 bp), CG genotype (219, 161, 81, 58, 34 and 13 bp), and GG genotype (219, 81, 34 and 13 bp)
Agarose gel electrophoresis (3%) of PCR-restriction fragment length polymorphism result for CATHL7/HinfI polymorphism. From left: pUC19/MspI DNA ladder (501/489, 404, 331, 242, 190, 147, 111/110, 67 and 34 base pairs (bp)), CC genotype (161, 81, 58, 34 and 14 bp), CG genotype (219, 161, 81, 58, 34 and 13 bp), and GG genotype (219, 81, 34 and 13 bp)

PCR conditions, product size, restriction enzyme, and fragment size after restriction endonuclease digestion

SNP PCR Conditions Product size Restriction enzyme Genotype Fragment size after digestion
initial: 95ºC/5 min CC
 324, 23 bp
2,383C > G
 HhaI
 CG
 347, 324, 23 bp
35 cycles: denaturation:95ºC/30 s GG
 347 bp
annealing: 52ºC/45 s extension:72ºC/30 s 347 bp CC
 161, 81, 58, 34, 14 bp
2,468 C > G HinfI CG
 219, 161, 81, 58, 34, 13 bp
final extension: 72ºC/7 min GG
 219, 81, 34, 13 bp

Means and standard deviation of traits in relation to genotypes

N Genotype Milk yield kg Fat content % Protein content % Lactose content % LnSCC
CATHL7/HhaI
138 CC 33.93A ± 7.86 3.84A ± 0.72 3.52A ± 0.33 4.95AB ± 0.16 4.18A ± 0.82
68 CG 33.81B ± 8.18 3.80B ± 0.75 3.52B ± 0.35 4.89B ± 0.17 4.28 ± 0.84
17
 GG
 30.42AB ± 6.29
 4.07AB ± 0.70
 3.59AB ± 0.37
 4.90A ± 0.16
 4.34A ± 0.75
CATHL7/HinfI
117 CC 33.48 ± 7.59 3.87 ± 0.71 3.53A ± 0.34 4.94a ± 0.16 4.18 ± 0.85
91 CG 33.87 ± 8.34 3.81 ± 0.77 3.51a ± 0.34 4.91a ± 0.16 4.27 ± 0.81
15 GG 34.42 ± 7.89 3.80 ± 0.67 3.47Aa ± 0.31 4.92 ± 0.17 4.25 ± 0.73

Genotype and allele frequencies of CATHL7

N Genotype frequencies Allele frequencies χ2
169 CC 0.606
CATHL7/HhaI
 91 CG 0.326 C 0.769 1.897
19
 GG
 0.068
 G
 0.231
144 CC 0.516
CATHL7/HinfI 115 CG 0.412 C 0.722 0.208
20 GG 0.072 G 0.278
eISSN:
2450-8608
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
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