<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2023-0004_s_005"><h3>Introduction</h3>
<p><italic>Escherichia coli</italic> is the most common pathogen isolated from the urine of dogs with urinary tract infections (UTIs). While there are many studies in humans investigating the potential for the prevention of UTIs by dietary consumption of cranberry, few analogous studies have been carried out in dogs.</p></sec>
<sec id="j_jvetres-2023-0004_s_006"><h3>Material and Methods</h3>
<p>Eight dogs, four male and four female, were successively fed two diets, first a control without cranberry, and then the second diet containing cranberry extracts. Naturally excreted urine was collected on the tenth day after the start of each diet for 24 h and used for bacterial growth. Madin-Darby canine kidney cell adherence by the uropathogenic <italic>E. coli</italic> G1473 strain expressing type 1 pili and positive for P pili and haemolysin gene markers was quantified after growth in urine samples.</p></sec>
<sec id="j_jvetres-2023-0004_s_007"><h3>Results</h3>
<p>Significant reductions in bacterial adherence to MDCK cells (from −16.5 to −73.4%, P &lt; 0.05) were observed in the four females but not in the males after consumption of the cranberry extracts compared to the same animals consuming the control diet.</p></sec>
<sec id="j_jvetres-2023-0004_s_008"><h3>Conclusion</h3>
<p>Dietary supplementation with cranberry may provide some degree of protection to female dogs against adhesion of uropathogenic <italic>E. coli</italic> to urinary epithelial cells.</p></sec>
</abstract>

Introduction
Escherichia coli is the most common pathogen isolated from the urine of dogs with urinary tract infections (UTIs). While there are many studies in humans investigating the potential for the prevention of UTIs by dietary consumption of cranberry, few analogous studies have been carried out in dogs.
Material and Methods
Eight dogs, four male and four female, were successively fed two diets, first a control without cranberry, and then the second diet containing cranberry extracts. Naturally excreted urine was collected on the tenth day after the start of each diet for 24 h and used for bacterial growth. Madin-Darby canine kidney cell adherence by the uropathogenic E. coli G1473 strain expressing type 1 pili and positive for P pili and haemolysin gene markers was quantified after growth in urine samples.
Results
Significant reductions in bacterial adherence to MDCK cells (from −16.5 to −73.4%, P < 0.05) were observed in the four females but not in the males after consumption of the cranberry extracts compared to the same animals consuming the control diet.
Conclusion
Dietary supplementation with cranberry may provide some degree of protection to female dogs against adhesion of uropathogenic E. coli to urinary epithelial cells.

Adherence of uropathogenic <italic>Escherichia coli</italic> in dog urine after consumption of food supplemented with cranberry (<italic>Vaccinium macrocarpon</italic>)

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Adherence of uropathogenic  in dog urine after consumption of food supplemented with cranberry ()

Introduction
Escherichia coli is the most common pathogen isolated from the urine of dogs with urinary tract infections (UTIs). While there are many studies in...

Sex	Dogs	Urine samples	Mean	SD	P Student’s t test
Male	1	U1	4.22	2.91	0.055
	1	UCR1	5.78	4.42	0.055
	2	U2	4.5	1.54	0.51
	2	UCR2	5.02	3.53	0.51
	3	U3	3.07	0.11	0.57
	3	UCR3	3.55	1.76	0.57
	4	U4	3.25	1.06	0.009
	4	UCR4	3.92	2.04	0.009
Female	5	U5	7.89	4.33	1.34 10^-37
	5	UCR5	2.10	1.62	1.34 10^-37
	6	U6	6.75	3.45	4.97 10^-17
	6	UCR6	3.38	2.05	4.97 10^-17
	7	U7	11.04	7.89	1.67 10^-24
	7	UCR7	4.68	3.99	1.67 10^-24
	8	U8	5.47	2.75	0.028
	8	UCR8	4.57	2.43	0.028
Male + Female	1 to 8	U Total	5.65	2.39	1.1 10^-39
Male + Female	1 to 8	UCR Total	3.91	0.95	1.1 10^-39

Adherence of uropathogenic Escherichia coli in dog urine after consumption of food supplemented with cranberry (Vaccinium macrocarpon)

Data publikacji: 09 mar 2023

Zakres stron: 49 - 54

Otrzymano: 17 lip 2022

Przyjęty: 27 sty 2023

DOI: https://doi.org/10.2478/jvetres-2023-0004

Słowa kluczowe
dog, urinary tract infection, cranberry, adherence

© 2023 A. Carvajal-Campos et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Adherence indices of the G1473 UPEC strain to the MDCK cell-line

Adherence of uropathogenic Escherichia coli in dog urine after consumption of food supplemented with cranberry (Vaccinium macrocarpon)

Data publikacji: 09 mar 2023

Zakres stron: 49 - 54

Otrzymano: 17 lip 2022

Przyjęty: 27 sty 2023

DOI: https://doi.org/10.2478/jvetres-2023-0004

Słowa kluczowedog, urinary tract infection, cranberry, adherence

© 2023 A. Carvajal-Campos et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Adherence indices of the G1473 UPEC strain to the MDCK cell-line

Słowa kluczowe
dog, urinary tract infection, cranberry, adherence