Evaluation of a real-time PCR in combination with a cultivation method for the detection of Brucella abortus in organs of infected cattle in southern Italy
, , , , , , , , , , , oraz | 18 gru 2022
O artykule
Data publikacji: 18 gru 2022
Zakres stron: 559 - 563
Otrzymano: 18 maj 2022
Przyjęty: 26 paź 2022
DOI: https://doi.org/10.2478/jvetres-2022-0062
Słowa kluczowe
© 2022 V. Manzulli et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1
Results of serological, culture and real-time PCR analyses in organs of slaughtered cattle following a brucellosis outbreak
| Animal | Serological status | Lymph node |
Udder | Spleen | Cotyledon | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Submandibular |
Supramammary |
Retropharyngeal |
Internal iliac |
||||||||||||
| PCR | Culture | PCR | Culture | PCR | Culture | PCR | Culture | PCR | Culture | PCR | Culture | PCR | Culture | ||
| A | RBT+ / CFT+ | + (•) | + (•••) | − | − | + (•) | + (••) | + (••) | + (•••) | − | − | + (••) | + (•••) | Not | sampled |
| B | RBT+ / CFT+ | + (•) | + (•••) | − | − | − | − | + (hom) | + (••) | − | − | + (•) | + (•••) | Not | sampled |
| C | RBT+ / CFT+ | + (•) | + (••) | − | − | + (•) | + (••) | + (•) | + (••) | + (•) | + (•••) | + (•) | + (••) | + (•) | + (••) |
| D | RBT+ / CFT+ | + (•) | + (•••) | − | − | + (•) | + (••) | + (•) | + (••) | − | − | − | − | + (•) | + (••) |
| E | RBT+ / CFT+ | + (•) | + (••) | + (•) | + (••) | − | − | + (•) | + (••) | − | − | − | − | + (•) | + (••) |
| F | RBT+ / CFT+ | + (•) | + (•) | + (•) | + (••) | + (•) | + (••) | + (•) | + (•) | + (•) | + (••) | + (•) | + (••) | + (hom) | + (•) |
| G | RBT+ / CFT+ | + (•) | + (••) | − | − | + (•) | + (••) | + (•) | + (••) | − | − | + (•) | + (••) | Not | sampled |
| H | RBT+ / CFT− | + (•) | + (••) | + (•) | + (••) | + (•) | + (••) | + (•) | + (••) | − | − | − | − | − | − |
| I | RBT− / CFT− | + (••) | + (•••) | − | − | + (•) | + (••) | + (•) | + (••) | − | − | + (•) | + (••) | + (hom) | + (•) |
| L | RBT− / CFT− | + (•) | + (••) | − | − | + (hom) | + (••) | − | − | − | − | − | − | + (•) | + (••) |