Determination of anti-phage antibodies in calf sera following application of Escherichia coli and Mannheimia haemolytica -specific bacteriophages
Data publikacji: 12 sie 2022
Zakres stron: 353 - 360
Otrzymano: 17 sty 2022
Przyjęty: 21 lip 2022
DOI: https://doi.org/10.2478/jvetres-2022-0041
Słowa kluczowe
© 2022 R. Urban-Chmiel. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
The widespread occurrence of drug-resistant bacteria has increased interest in alternatives to antibiotics for combatting bacterial infections, among which bacteriophages play an important role. The ability of phage proteins to induce an anti-phage immune response can significantly limit the effectiveness of treatment, which was the basis for the study described in this article. The aim of the study was to assess the effects of bacteriophages on the induction of an anti-phage humoral response in calves.
Material and Methods
The study was conducted using phage components of experimental preparations and sera from calves treated and not treated with phages. Levels of G, M and A immunoglobulins were analysed by ELISA. The assay plates were coated with whole
Results
The results confirmed an increased anti-phage response affecting all immunoglobulin classes in the calf sera. The highest significant (P ≤ 0.05) level of antibodies was observed for IgG in the sera of calves receiving phages. The phage neutralisation test showed a significant differences (P ≤ 0.05) in the reduction of phage titres in comparison to untreated calves.
Conclusion
Despite the induction of an anti-phage response, no significant negative effect on the antibacterial activity of phages was observed