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Expression of bovine leukaemia virus (BLV) gp51 protein in blood and milk cells of cows with leukosis

Maria Szczotka
Maria Szczotka
 oraz 
Jacek Kuźmak
Jacek Kuźmak
   | 30 lip 2022
Journal of Veterinary Research's Cover Image
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Data publikacji: 30 lip 2022
Zakres stron: 305 - 315
Otrzymano: 29 gru 2021
Przyjęty: 24 cze 2022
DOI: https://doi.org/10.2478/jvetres-2022-0035
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© 2022 M. Szczotka, J. Kuźmak. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

The difference in the percentages of cluster of differentiation CD19+ and CD19+ immunoglobulin M+ B and CD4+ and CD8+ T lymphocyte subpopulations depending on bovine leukaemia virus infection stage AL – aleukaemic stage of infection; PL – persistently lymphocytotic stage of infection. Median values were used to provide a better measure of the central tendency
The difference in the percentages of cluster of differentiation CD19+ and CD19+ immunoglobulin M+ B and CD4+ and CD8+ T lymphocyte subpopulations depending on bovine leukaemia virus infection stage AL – aleukaemic stage of infection; PL – persistently lymphocytotic stage of infection. Median values were used to provide a better measure of the central tendency

Fig. 2

The effect of BLV gp51 antigen expression in the of cluster of differentiation CD19+ and CD19+ immunoglobulin M (IgM)+ B and CD4+ and CD8+ T lymphocyte subpopulations AL – aleukaemic stage of infection; PL – persistently lymphocytotic stage of infection
The effect of BLV gp51 antigen expression in the of cluster of differentiation CD19+ and CD19+ immunoglobulin M (IgM)+ B and CD4+ and CD8+ T lymphocyte subpopulations AL – aleukaemic stage of infection; PL – persistently lymphocytotic stage of infection

Fig. 3

The effect of BLV gp51 antigen expression in the number of cluster of differentiation CD19+ and CD19+ immunoglobulin M (IgM)+ B and CD4+ and CD8+ T lymphocyte subpopulations AL – aleukaemic stage of infection; PL – persistently lymphocytotic stage of infection
The effect of BLV gp51 antigen expression in the number of cluster of differentiation CD19+ and CD19+ immunoglobulin M (IgM)+ B and CD4+ and CD8+ T lymphocyte subpopulations AL – aleukaemic stage of infection; PL – persistently lymphocytotic stage of infection

Fig. 4

The expression of glycoprotein 51 in blood and milk lymphocytes of bovine leukaemia virus-infected cows with clearly much higher expression in blood lymphocytes than in milk cells
The expression of glycoprotein 51 in blood and milk lymphocytes of bovine leukaemia virus-infected cows with clearly much higher expression in blood lymphocytes than in milk cells

Fig. 5

The expression of BLV glycoprotein (gp) 51 antigen in milk: in cluster of differentiation CD19+, CD19+ immunoglobulin M (IgM)+ B and CD4+, CD8+ T lymphocyte subpopulations A – AL stage of infection; B – PL stage of infection
The expression of BLV glycoprotein (gp) 51 antigen in milk: in cluster of differentiation CD19+, CD19+ immunoglobulin M (IgM)+ B and CD4+, CD8+ T lymphocyte subpopulations A – AL stage of infection; B – PL stage of infection

Fig. 6

Immunofluorescence showing the expression of bovine leukaemia virus glycoprotein 51 in blood lymphocytes of a cow with persistent lymphocytosis
Immunofluorescence showing the expression of bovine leukaemia virus glycoprotein 51 in blood lymphocytes of a cow with persistent lymphocytosis

Fig. 7

Immunofluorescence showing the expression of bovine leukaemia virus glycoprotein51 in cells from a milk sample from a leukaemic cow
Immunofluorescence showing the expression of bovine leukaemia virus glycoprotein51 in cells from a milk sample from a leukaemic cow

The expression of gp51 in blood lymphocyte subsets of bovine leukaemia virus infected cattle (%)

Cow sample Stage of infection Lymphocytes/mm3 CD markers + gp51 (%)
 Cells with gp51 expression
CD19+ CD19+ IgM+ CD4+ CD8+
1 AL 7,600 15 20 0 16 51
2 7,800 25 7 1 7 40
3 8,400 29 20 0 10 59
4 8,800 38 12 2 10 62
5 9,000 30 18 3 21 72
6 9,600 38 13 0 17 68
7 9,600 1 3 0 2 6
8 10,200 24 10 0 1 35
9 10,300 3 3 0 0 6
10 10,400 28 19 3 12 62
11 11,200 1 1 0 0 2
Mean value 9,354 21.0 11.4 0.8 8.7 41.3
1 PL 12,800 16 10 1 4 31
2 15,200 21 24 0 17 62
3 15,600 19 30 0 5 54
4 26,000 31 9 0 2 42
5 26,400 9 8 1 5 23
6 39,000 34 23 2 2 61
7 39,000 28 18 0 2 58
8 63,200 68 20 1 4 93
Mean value 29,650 28.25 17.7 0.6 5.2 53.0

The expression of gp51 in milk lymphocyte subsets of bovine leukaemia virus–infected cows (%)

Cow sample Stage of infection Lymphocytes/mm3 CD markers (%)
 Expression of gp51 in milk lymphocytes (%)
ELISA PCR CD19+ CD4+ CD8+ CD19+ IgM+
1 AL 7,600 + + 1 − − 1 2
2 7,800 + + 2 − − − 2
3 8,400 + + − − − 3 3
4 8,800 + + − − − 2 2
5 9,000 + + 2 − 1 − 3
6 9,600 + + 1 − 1 − 2
7 9,600 + + 1 − − 3 4
8 10,200 + + 1 − 1 1 3
9 10,300 + + 3 − − − 3
10 10,400 + + 18 − − 4 21
11 11,200 + + 10 − − 6 20
Mean value 9,354 + + 3.5 - 0.3 1.8 5.7
1 PL 12,800 + + 1 − − 1 2
2 15,200 + + 3 − − 2 5
3 15,600 + + 1 − − 2 3
4 26,000 + + 2 − 1 − 3
5 26,400 + + 9 1 2 3 15
6 39,000 + + 4 − − 3 7
7 39,000 + + 10 − 1 2 13
8 63,200 + + 21 − 4 9 33
Mean value 29,650 6.3 0.1 1.0 2.7 10.2

The percentage of peripheral blood lymphocyte subpopulations of bovine leukaemia virus–infected cattle in the aleukaemic (AL) and persistently lymphocytotic (PL) stages

Cow sample ELISA PCR Phase of infection Lymphocytes/mm3 CD19+ CD19+ IgM+ CD4+ CD8+
1 + + AL 7,600 25 46 25 3
2 + + 7,800 67 75 15 17
3 + + 8,400 54 80 27 23
4 + + 8,800 45 27 31 10
5 + + 9,000 54 45 26 32
6 + + 9,600 54 61 23 27
7 + + 9,600 67 64 33 20
8 + + 10,200 47 18 30 6
9 + + 10,300 82 68 1 2
10 + + 10,400 96 20 2 2
11 + + 11,200 51 19 30 4
Mean value 9,354 58.3 47.5 22.1 13.5
1 + + PL 12,800 66 65 21 4
2 + + 15,200 55 77 27 31
3 + + 15,600 39 62 13 5
4 + + 26,000 83 81 6 2
5 + + 26,400 61 81 5 2
6 + + 39,000 96 61 8 2
7 + + 39,000 73 36 7 2
8 + + 63,200 96 86 4 4
Mean value 29,650 71.1 68.6 11.4 6.5
eISSN:
2450-8608
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
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