Cytogenetic karyotype analysis in selected species of the Erinaceidae family
Data publikacji: 13 wrz 2019
Zakres stron: 353 - 358
Otrzymano: 21 gru 2019
Przyjęty: 02 lip 2019
DOI: https://doi.org/10.2478/jvetres-2019-0041
Słowa kluczowe
© 2019 A. Grzesiakowska et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Comparative analysis of the karyotype structure was made in two hedgehog species: the northern white-breasted hedgehog (
Material and Methods
The cytogenetic analysis used differential staining techniques (DAPI, Ag-NOR, and C-banding/DAPI) and sequential QFQ/FISH banding with NOR20 and TEL20 probes which showed 45S rDNA and (TTAGGG)n repeat sequences, respectively, on hedgehog chromosomes.
Results
It was confirmed that the somatic cells of the hedgehogs have a constant number of chromosomes (2n = 48,XY). Differences were observed in the NOR number between the species. NORs were identified on three autosome pairs in the northern white-breasted hedgehog and on only two pairs in the African pygmy hedgehog. Chromosome analysis by C-banding/DAPI showed large segments of heterochromatin rich in A-T pairs on three autosome pairs in both the northern white-breasted and African pygmy hedgehogs. The heterochromatin segments encompassed large fragments of the longer arm of chromosome pairs 13, 14 and 20. The (TTAGGG)n repeat sequences on the hedgehog chromosomes were only observed in the terminal position of all the chromosomes in both species.
Conclusion
Our observations provide new information on the level of diversity within the