Validation of the Analytical Method for the Determination of Flavonoids in Broccoli

Australian Pesticides & Veterinary Medicines Authority (APVMA). Guidelines for the validation of analy–tical methods for active constituent, agricultural and veterinary chemical products. October 2004.Search in Google Scholar

De Rijke E., Out P., Niessen W.M.A., Ariese F., Gooijer C., Brinkman U.A.T. 2006. Analytical separation and detection methods for flavonoids. J. Chromatogr. A 1112: 31-63. DOI: 10.1016/j.chroma.2006.01.019.10.1016/j.chroma.2006.01.019Search in Google Scholar

Dixon R., Paiva N. 1995. Stress-induced phenylpro-panoid metabolism. Plant Cell 7: 1085-1097. DOI: 10.1105/tpc.7.7.1085.10.1105/tpc.7.7.1085Search in Google Scholar

Duthie G., Crozier A. 2000. Plant derived phenolic anti-oxidants. Curr. Opin. Lipidol. 11: 43-47.10.1097/00041433-200002000-00007Search in Google Scholar

Erlund I. 2004. Review of the flavonoids quercetin, hes-peretin, and naringenin. Dietary sources, bioactivi-ties, bioavailability, and epidemiology. Nutr. Res. 24: 851-874. DOI: 10.1016/j.nutres.2004.07.005.10.1016/j.nutres.2004.07.005Search in Google Scholar

Gliszczyńska-Świgło A., Ciska E., Pawlak-Lemańska K., Chmielewski J., Borkowski T., Tyrakowska B. 2006. Changes in the content of health-promoting com–pounds and antioxidant activity of broccoli after do-mestic processing. Food Addit. Contam. 23(11): 1088-1098. DOI: 10.1080/02652030600887594.10.1080/02652030600887594Search in Google Scholar

Hertog M.G.L., Hollman P.C.H., Venema D.P. 1992. Optimization of a quantitative HPLC determina–tion of potentially anticarcinogenic flavonoids in vegetables and fruits. J. Agric. Food Chem. 40: 1591-1598.10.1021/jf00021a023Search in Google Scholar

Horbowicz M. 1999. Changes of flavonols content in onion during the vegetation period and storage. Veget. Crops Res. Bull. 50: 81-91.Search in Google Scholar

International Conference on Harmonization (ICH). “Validation of Analytical Procedures -PA/PH/OMCL (05) 47 DEF”, elaborated by OMCL Network/EDQM of the Council of Europe; June 2005.Search in Google Scholar

Koh E., Wimalasiri K.M.S., Chassy A.W., Mitchell A.E. 2009. Content of ascorbic acid, quercetin, kaempferol and total phenolics in commercial broccoli. J. Food Com. Anal. 22: 637-643. DOI: 10.1016/j.jfca.2009.01.019.10.1016/j.jfca.2009.01.019Search in Google Scholar

Manach C., Mazur A., Scalbert A. 2005. Polyphe–nols and prevention of cardiovascular dis–eases. Curr. Opin. Lipidol. 16: 77-84. DOI: 10.1097/00041433-200502000-00013.10.1097/00041433-200502000-00013Search in Google Scholar

Merken H.M., Beecher G.R. 2000. Liquid chromato-graphic method for the separation and quantifi–cation of prominent flavonoid aglycones. J. Chromatogr. A 897: 177-184. DOI: 10.1016/S0021-9673(00)00826-8.10.1016/S0021-9673(00)00826-8Search in Google Scholar

Middleton E. Jr., Kandaswami C., Theoharides T.C. 2000. The effects of plant flavonoids on mamma–lian cells: implications for inflammation, heart dis–ease, and cancer. Pharmacol. Rev. 52(4): 673-751.Search in Google Scholar

Nijveldt R.J., van Nood E., van Hoorn D.E.C., Boe-lens P.G., van Norren K., van Leeuwen P.A.M. 2001. Flavonoids: a review of probable me–chanisms of action and potential applications. Am. J. Clin. Nutr. 74: 418-425.10.1093/ajcn/74.4.41811566638Search in Google Scholar

Nuutila A.M., Kammiovirta K., Oksman-Caldentey K.M. 2002. Comparison of methods for the hydrol–ysis flavonoids and phenolic acids from onion and spinach for HPLC analysis. Food Chem. 76(4): 519-525. DOI: 10.1016/S0308-8146(01)00305-3.10.1016/S0308-8146(01)00305-3Search in Google Scholar

Patil B.S., Pike L.M., Yoo K.S. 1995. Variation in the quercetin content in different colored onions (Allium cepa L.). J. Am. Soc. Hort. Sci. 120(6): 909-913.Search in Google Scholar

Perez-Vizcaino F., Bishop-Bailley D., Lodi F., Duarte J., Cogolludo A., Moreno L., Bosca L., Mitchell J.A., Warner T.D. 2006. The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells. Biochem. Biophys. Res. Commun. 346(3): 919-925.Search in Google Scholar

Pietta P.G. 2000. Flavonoids as antioxidants. J. Nat. Prod. 63(7): 1035-1042. DOI: 10.1021/np9904509.10.1021/np9904509Search in Google Scholar

Price K.R., Casuscelli F., Colquhoun I.J., Rhodes M.J.C. 1998. Composition and content of flavonol glycosides in broccoli florets (Brassica olerácea) and their fate during cooking. J. Sci. Food Agr. 77(4): 468-472.10.1002/(SICI)1097-0010(199808)77:4<468::AID-JSFA66>3.0.CO;2-BSearch in Google Scholar

Repolles C., Herrero-Martinez J.M., Ràfolds C. 2006. Analysis of prominent flavonoid aglycones by high-performance liquid chromatography using a monolithic column. J. Chromatogr. A 1131: 51-57. DOI: 10.1016/j.chroma.2006.07.012.10.1016/j.chroma.2006.07.012Search in Google Scholar

Robards K. 2003. Strategies for the determination of bi-oactive phenols in plants, fruit and vegetables. J. Chromatogr. A 1000(1-2): 657-691. DOI: 10.1016/S0021-9673(03)00058-X.10.1016/S0021-9673(03)00058-XSearch in Google Scholar

Rodríguez-Delgado M.A., Malovaná S., Pérez J.P., Borges T., García Montelongo F.J. 2001. Separa–tion of phenolic compounds by high-performance liquid chromatography with absorbance and fluo-rimetric detection. J. Chromatogr. A 912(2): 249–257. DOI: 10.1016/S0021-9673(01)00598-2.10.1016/S0021-9673(01)00598-2Search in Google Scholar

Rossi M., Garavello W., Talamini R., La Vecchia C., Franceschi S., Lagiou P., Zambon P., Dal Maso L., Bosetti C., Negri E. 2007. Flavonoids and risk of squamous cell esophageal cancer. Int. J. Can–cer. 120(7): 1560-1564. DOI: 10.1002/ijc.22499.10.1002/ijc.22499Search in Google Scholar

Roy M.K., Juneja L.R., Isobe S., Tsushida T. 2009. Steam processed broccoli (Brassica oleracea) has higher antioxidant activity in chemical and cellu–lar assay systems. Food Chem. 114(1): 263-269. DOI: 10.1016/j.foodchem.2008.09.050.10.1016/j.foodchem.2008.09.050Search in Google Scholar

Schieber A., Keller P., Carle R. 2001. Determination of phenolic acids and flavonoids of apple and pear by high-performance chromatography. J. Chromatogr. A 910(2): 265-273. DOI: 10.1016/S0021-9673(00)01217-6.10.1016/S0021-9673(00)01217-6Search in Google Scholar

Schroeter H., Heiss C., Balzer J., Kleinbongard P., Keen C.L., Hollenberg N.K., Sies H., Kwik-Uribe C., Schmitz H., Kelm M. 2006. (-)-Epicatechin mediates beneficial effects of flavonol-rich cocoa on vascular function in humans. Proc. Natl. Acad. Sci. USA 103(4): 1024-1029. DOI: 10.1073/pnas.0510168103.10.1073/pnas.0510168103Search in Google Scholar

Sellappan S., Akoh C.C. 2002. Flavonoids and antioxi–dant capacity of Georgia-grown Vidalia onions. J. Agric. Food Chem. 50(19): 5338-5342. DOI: 10.1021/jf020333a.10.1021/jf020333aSearch in Google Scholar

Sousa de Brito E., Pessanha de Araújo M.C., Lin L.Z., Harnly J. 2007. Determination of the flavonoid components of cashew apple (Anacardium occi–dentale) by LC-DAD-ESI/MS. Food Chem. 105(3): 1112-1118. DOI: 10.1016/j.foodchem.2007.02.009.10.1016/j.foodchem.2007.02.009Search in Google Scholar

Steinmetz K.A., Potter J.D. 1996. Vegetables, fruit, and cancer prevention: a review. J. Am. Diet. Assoc. 96(10): 1027-1039. DOI: 10.1016/S0002-8223(96)00273-8.10.1016/S0002-8223(96)00273-8Search in Google Scholar

Vallejo F., Tomás-Barberán F.A., García-Viguera C. 2003. Health-promoting compounds in broccoli as influenced by refrigerated transport and retail sale period. J. Agric. Food Chem. 51(10): 3029–3034. DOI: 10.1021/jf021065j.10.1021/jf021065j12720387Search in Google Scholar

Vallejo F., Tomás-Barberán F.A., García-Viguera C. 2002. Potential bioactive compounds in health promotion from broccoli cultivars grown in Spain. J. Sci. Food Agric. 82(11): 1293-1297. DOI: 10.1002/jsfa.1183.10.1002/jsfa.1183Search in Google Scholar

Van Acker S.A.B.E., Tromp M.N.J.L., Haenen G.R.M.M., van der Vijgh W.J.F., Bast A. 1995. Flavonoids as scavengers of nitric oxide radical. Biochem. Biophys. Res. Commun. 214(3): 755-759.Search in Google Scholar

Zu Y., Li C., Fu Y., Zhao C. 2006. Simultaneous deter–mination of catechin, rutin, quercetin, kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD. J. Pharm. Biomed. Anal. 41(3): 714–719. DOI: 10.1016/j.jpba.2005.04.052.10.1016/j.jpba.2005.04.05216520013Search in Google Scholar