Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay
, oraz
30 lip 2013
O artykule
Data publikacji: 30 lip 2013
Zakres stron: 11 - 14
DOI: https://doi.org/10.2478/johr-2013-0002
Słowa kluczowe
This content is open access.
A one-step RT-PCR procedure was developed for specific detection of Narcissus latent virus (NLV) isolates. Following alignment of RNA sequences of three NLV isolates, the conserved sequence fragments were identified in viral polyprotein gene and 3’UTR region. Based on those fragments, a forward NLs1 and two reverse: NLa1 and NLSCPR2 primers were designed. Primer pairs NLs1-NLa1 and NLs1- NLSCPR2 proved to be effective in amplification of a 669 bp and 1295 bp products, respectively. Sequence analysis of amplified products confirmed their specificity.