Exploring the use of metabarcoding to reveal eukaryotic associations with mononchids nematodes

Adl, S. M., Simpson, A. G. B., Lane, C. E., Lukeš, J., Bass, D., Bowser, S. S., Brown, M. W., Burki, F., Dunthorn, M., Hampl, V., Heiss, A., Hoppenrath, M., Lara, E., Gall, L. L., Lynn, D. H., Mcmanus, H., Mitchell, E. A. D., Mozley-Stanridge, S. E., Parfrey, L. W, Pawlowski, J., Rueckert, S., Shadwick, L., Schoch, C. L., Smirnov, A. and Spiegel, F. W. 2012. The revised classification of eukaryotes. Journal of Eukaryotic Microbiology 59:429–514. DOI: 10.1111/j.1550-7408.2012.00644.x. AdlS. M. SimpsonA. G. B. LaneC. E. LukešJ. BassD. BowserS. S. BrownM. W. BurkiF. DunthornM. HamplV. HeissA. HoppenrathM. LaraE. GallL. L. LynnD. H. McmanusH. MitchellE. A. D. Mozley-StanridgeS. E. ParfreyL. W PawlowskiJ. RueckertS. ShadwickL. SchochC. L. SmirnovA. SpiegelF. W. 2012 The revised classification of eukaryotes Journal of Eukaryotic Microbiology 59 429 514 10.1111/j.1550-7408.2012.00644.x Open DOISearch in Google Scholar

Arpin, P. and Kilbertus, G. 1981. Ultrastructure du contenu digestif et de l’épithelium intestinal chez quelques nématodes prédateurs (Mononchida) et bactériophages. Revue Nematology 4:131–143. ArpinP. KilbertusG. 1981 Ultrastructure du contenu digestif et de l’épithelium intestinal chez quelques nématodes prédateurs (Mononchida) et bactériophages Revue Nematology 4 131 143 Search in Google Scholar

Berg, M., Stenuit, B., Ho, J., Wang, A., Parke, C., Knight, M., Alvarez-Cohen, L. and Shapira, M. 2016. Assembly of the Caenorhabditis elegans gut microbiota from diverse soil microbial environments. ISME Journal 10:1998–2009. DOI: 10.1038/ismej.2015.253. BergM. StenuitB. HoJ. WangA. ParkeC. KnightM. Alvarez-CohenL. ShapiraM. 2016 Assembly of the Caenorhabditis elegans gut microbiota from diverse soil microbial environments ISME Journal 10 1998 2009 10.1038/ismej.2015.253 Open DOISearch in Google Scholar

Bilgrami, A. L. 2008. Biological control potentials of predatory nematodes. Integrated Management and Biocontrol of Vegetable and Grain Crops Nematodes 3:28. DOI: 10.1007/978-1-4020-6063-2_1. BilgramiA. L. 2008 Biological control potentials of predatory nematodes Integrated Management and Biocontrol of Vegetable and Grain Crops Nematodes 3 28 10.1007/978-1-4020-6063-2_1 Open DOISearch in Google Scholar

Bilgrami, A. L., Ahmad, I. and Shamim, J. M. 1986. A study of the intestinal contents of some mononchs. Revue de Nématologie 9:191–194. BilgramiA. L. AhmadI. ShamimJ. M. 1986 A study of the intestinal contents of some mononchs Revue de Nématologie 9 191 194 Search in Google Scholar

Cabos, R. Y. M., Wang, K. H., Sipes, B. S., Heller, W. P. and Matsumoto, T. K. 2013. Detection of plant-parasitic nematode DNA in the gut of predatory and omnivorous nematodes. Nematropica 43:44–48. CabosR. Y. M. WangK. H. SipesB. S. HellerW. P. MatsumotoT. K. 2013 Detection of plant-parasitic nematode DNA in the gut of predatory and omnivorous nematodes Nematropica 43 44 48 Search in Google Scholar

Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A. and Holmes, S. P. 2016. DADA2: High-resolution sample inference from Illumina amplicon data. Nature Methods 13:581–583. DOI: 10.1038/nmeth.3869. CallahanB. J. McMurdieP. J. RosenM. J. HanA. W. JohnsonA. J. A. HolmesS. P. 2016 DADA2: High-resolution sample inference from Illumina amplicon data Nature Methods 13 581 583 10.1038/nmeth.3869 Open DOISearch in Google Scholar

Casper, R. M., Jarman, S. N., Gales, N. J. and Hindell, M. A. 2007. Combining DNA and morphological analyses of fecal samples improves insight into trophic interactions: A case study using a generalist predator. Marine Biology 152:815–825. DOI: 10.1007/s00227-007-0732-y. CasperR. M. JarmanS. N. GalesN. J. HindellM. A. 2007 Combining DNA and morphological analyses of fecal samples improves insight into trophic interactions: A case study using a generalist predator Marine Biology 152 815 825 10.1007/s00227-007-0732-y Open DOISearch in Google Scholar

Chen, S., Zhou, Y., Chen, Y. and Gu, J. 2018. Fastp: An ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34:i884–i890. DOI: 10.1093/bioinformatics/bty560. ChenS. ZhouY. ChenY. GuJ. 2018 Fastp: An ultra-fast all-in-one FASTQ preprocessor Bioinformatics 34 i884 i890 10.1093/bioinformatics/bty560 Open DOISearch in Google Scholar

Cobb, N. A. 1917. The Mononchs: a genus of free-living predatory nematodes. Soil Science 3:431–486. DOI: 10.1097/00010694-191705000-00004. CobbN. A. 1917 The Mononchs: a genus of free-living predatory nematodes Soil Science 3 431 486 10.1097/00010694-191705000-00004 Open DOISearch in Google Scholar

Deagle, B. E., Kirkwood, R. and Jarman, S. N. 2009. Analysis of Australian fur seal diet by pyrosequencing prey DNA in feces. Molecular Ecology 18:2022–2038. DOI: 10.1111/j.1365-294X.2009.04158.x. DeagleB. E. KirkwoodR. JarmanS. N. 2009 Analysis of Australian fur seal diet by pyrosequencing prey DNA in feces Molecular Ecology 18 2022 2038 10.1111/j.1365-294X.2009.04158.x Open DOISearch in Google Scholar

Derycke, S., De Meester, N., Rigaux, A., Creer, S., Bik, H., Thomas, W. K. and Moens, T. 2016. Coexisting cryptic species of the Litoditis marina complex (Nematoda) show differential resource use and have distinct microbiomes with high intraspecific variability. Molecular Ecology 25:2093–2110. DOI: 10.1111/mec.13597. DeryckeS. De MeesterN. RigauxA. CreerS. BikH. ThomasW. K. MoensT. 2016 Coexisting cryptic species of the Litoditis marina complex (Nematoda) show differential resource use and have distinct microbiomes with high intraspecific variability Molecular Ecology 25 2093 2110 10.1111/mec.13597 Open DOISearch in Google Scholar

Engelbrektson, A., Kunin, V., Wrighton, K. C., Zvenigorodsky, N., Chen, F., Ochman, H. and Hugenholtz, P. 2010. Experimental factors affecting PCR-based estimates of microbial species richness and evenness. ISME Journal 4:642–647. DOI: 10.1038/ismej.2009.153. EngelbrektsonA. KuninV. WrightonK. C. ZvenigorodskyN. ChenF. OchmanH. HugenholtzP. 2010 Experimental factors affecting PCR-based estimates of microbial species richness and evenness ISME Journal 4 642 647 10.1038/ismej.2009.153 Open DOISearch in Google Scholar

Euringer, K. and Lueders, T. 2008. An optimized PCR/T-RFLP fingerprinting approach for the investigation of protistan communities in groundwater environments. Journal of Microbiological Methods 75:262–268. DOI: 10.1016/j.mimet.2008.06.012. EuringerK. LuedersT. 2008 An optimized PCR/T-RFLP fingerprinting approach for the investigation of protistan communities in groundwater environments Journal of Microbiological Methods 75 262 268 10.1016/j.mimet.2008.06.012 Open DOISearch in Google Scholar

Ferris, H., Bongers, T. and de Goede, R. G. M. 2001. A framework for soil food web diagnostics: extension of the nematode faunal analysis concept. Applied Soil Ecology 18:13–29. DOI: 10.1016/s0929-1393(01)00152-4. FerrisH. BongersT. de GoedeR. G. M. 2001 A framework for soil food web diagnostics: extension of the nematode faunal analysis concept Applied Soil Ecology 18 13 29 10.1016/s0929-1393(01)00152-4 Open DOISearch in Google Scholar

Hall, M. and Beiko, R. G. 2018. 16S rRNA Gene Analysis with QIIME2. in: R. Beiko, W. Hsiao, J. Parkinson, eds. Microbiome Analysis. Methods in Molecular Biology. V. 1849. New York: Humana Press. DOI: 10.1007/978-1-4939-8728-3_8. HallM. BeikoR. G. 2018 16S rRNA Gene Analysis with QIIME2 in: BeikoR. HsiaoW. ParkinsonJ. eds. Microbiome Analysis. Methods in Molecular Biology. V. 1849 New York Humana Press 10.1007/978-1-4939-8728-3_8 Open DOISearch in Google Scholar

Harper, G. L., King, R. A., Dodd, C. S., Harwood, J. D., Glen, D. M., Bruford, M. W. and Symondson, W. O. C. 2005. Rapid screening of invertebrate predators for multiple prey DNA targets. Molecular Ecology 14:819–827. DOI: 10.1111/j.1365-294x.2005.02442.x. HarperG. L. KingR. A. DoddC. S. HarwoodJ. D. GlenD. M. BrufordM. W. SymondsonW. O. C. 2005 Rapid screening of invertebrate predators for multiple prey DNA targets Molecular Ecology 14 819 827 10.1111/j.1365-294x.2005.02442.x Open DOISearch in Google Scholar

Khan, Z. and Kim, Y. H. 2005. The predatory nematode, Mononchoides fortidens (Nematoda: Diplogasterida), suppresses the root-knot nematode, Meloidogyne arenaria, in potted field soil. Biological Control 35:78–82. DOI: 10.1016/j.biocontrol.2005.05.015. KhanZ. KimY. H. 2005 The predatory nematode, Mononchoides fortidens (Nematoda: Diplogasterida), suppresses the root-knot nematode, Meloidogyne arenaria, in potted field soil Biological Control 35 78 82 10.1016/j.biocontrol.2005.05.015 Open DOISearch in Google Scholar

King, R. A., Vaughan, I. P., Bell, J. R., Bohan, D. A. and Symondson, W. O. C. 2010. Prey choice by carabid beetles feeding on an earthworm community analyzed using species- and lineage-specific PCR primers. Molecular Ecology 19:1721–1732. DOI: 10.1111/j.1365-294X.2010.04602.x. KingR. A. VaughanI. P. BellJ. R. BohanD. A. SymondsonW. O. C. 2010 Prey choice by carabid beetles feeding on an earthworm community analyzed using species- and lineage-specific PCR primers Molecular Ecology 19 1721 1732 10.1111/j.1365-294X.2010.04602.x Open DOISearch in Google Scholar

Martin, M. 2011. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet Journal 17:10–12. MartinM. 2011 Cutadapt removes adapter sequences from high-throughput sequencing reads EMBnet Journal 17 10 12 Search in Google Scholar

McQueen, J. P., Gattoni, K., Gendron, E. M. S., Schmidt, S. K., Sommers, P. and Porazinska, D. L. 2022. Host identity is the dominant factor in the assembly of nematode and tardigrade gut microbiomes in Antarctic Dry Valley streams. Scientific Reports 12. DOI: 10.1038/s41598-022-24206-5. McQueenJ. P. GattoniK. GendronE. M. S. SchmidtS. K. SommersP. PorazinskaD. L. 2022 Host identity is the dominant factor in the assembly of nematode and tardigrade gut microbiomes in Antarctic Dry Valley streams Scientific Reports 12 10.1038/s41598-022-24206-5 Open DOISearch in Google Scholar

McQueen, J. P., Gattoni, K., Gendron, E. M. S., Schmidt, S. K., Sommers, P. and Porazinska, D. L. 2023. External and internal microbiomes of Antarctic nematodes are distinct, but more similar to each other than the surrounding environment. Journal of Nematology 55. DOI: 10.2478/jofnem-2023-0004. McQueenJ. P. GattoniK. GendronE. M. S. SchmidtS. K. SommersP. PorazinskaD. L. 2023 External and internal microbiomes of Antarctic nematodes are distinct, but more similar to each other than the surrounding environment Journal of Nematology 55 10.2478/jofnem-2023-0004 Open DOISearch in Google Scholar

Mikaeili, F., Kia, E.B., Sharbatkhori, M., Sharifdini, M., Jalalizand, N., Heidari, Z., Zarei, Z., Stensvold, C.R., et al. (2013). Comparison of six simple methods for extracting ribosomal and mitochondrial DNA from Toxocara and Toxascaris nematodes. Experimental Parasitology 134:155–159. DOI: 10.1016/j.exppara.2013.02.008. MikaeiliF. KiaE.B. SharbatkhoriM. SharifdiniM. JalalizandN. HeidariZ. ZareiZ. StensvoldC.R. 2013 Comparison of six simple methods for extracting ribosomal and mitochondrial DNA from Toxocara and Toxascaris nematodes Experimental Parasitology 134 155 159 10.1016/j.exppara.2013.02.008 Open DOISearch in Google Scholar

Moens, T. and Vincx, M. 1998. On the cultivation of free-living marine and estuarine nematodes. Helgoländer Meeresuntersuchungen 52:115–139. MoensT. VincxM. 1998 On the cultivation of free-living marine and estuarine nematodes Helgoländer Meeresuntersuchungen 52 115 139 Search in Google Scholar

Pompanon, F., Deagle, B. E., Symondson, W. O. C., Brown, D. S., Jarman, S. N. and Taberlet, P. 2012. Who is eating what: Diet assessment using next-generation sequencing. Molecular Ecology 21:1931–1950. DOI: 10.1111/j.1365-294X.2011.05403.x. PompanonF. DeagleB. E. SymondsonW. O. C. BrownD. S. JarmanS. N. TaberletP. 2012 Who is eating what: Diet assessment using next-generation sequencing Molecular Ecology 21 1931 1950 10.1111/j.1365-294X.2011.05403.x Open DOISearch in Google Scholar

Pons, J. 2006. DNA-based identification of preys from non-destructive, total DNA extractions of predators using arthropod universal primers. Molecular Ecology Notes 6:623–626. DOI: 10.1111/j.1471-8286.2006.01353.x. PonsJ. 2006 DNA-based identification of preys from non-destructive, total DNA extractions of predators using arthropod universal primers Molecular Ecology Notes 6 623 626 10.1111/j.1471-8286.2006.01353.x Open DOISearch in Google Scholar

Porazinska, D. L., Giblin-Davis, R. M., Faller, L., Farmerie, W., Kanzaki, N., Morris, K., Powers, T. O., Tucker, A. E., Sung, W. and Thomas, K. 2009. Evaluating high-throughput sequencing as a method for metagenomic analysis of nematode diversity. Molecular Ecology Resources 9:1439–1450. DOI: 10.1111/j.1755-0998.2009.02611.x. PorazinskaD. L. Giblin-DavisR. M. FallerL. FarmerieW. KanzakiN. MorrisK. PowersT. O. TuckerA. E. SungW. ThomasK. 2009 Evaluating high-throughput sequencing as a method for metagenomic analysis of nematode diversity Molecular Ecology Resources 9 1439 1450 10.1111/j.1755-0998.2009.02611.x Open DOISearch in Google Scholar

Ruess, L., Häggblom, M. M., Garcia Zapata, J. and Dighton, J. 2002. Fatty acids of fungi and nematodes-possible biomarkers in the soil food chain? Soil Biology and Biochemistry 34:745–756. DOI: 10.1016/s0038-0717(01)00231-0. RuessL. HäggblomM. M. Garcia ZapataJ. DightonJ. 2002 Fatty acids of fungi and nematodes-possible biomarkers in the soil food chain? Soil Biology and Biochemistry 34 745 756 10.1016/s0038-0717(01)00231-0 Open DOISearch in Google Scholar

Salinas, K. A. and Kotcon, J. 2005. In vitro culturing of the predatory soil nematode Clarkus papillatus. Nematology 7:5–9. DOI: 10.1163/1568541054192162. SalinasK. A. KotconJ. 2005 In vitro culturing of the predatory soil nematode Clarkus papillatus Nematology 7 5 9 10.1163/1568541054192162 Open DOISearch in Google Scholar

Schuelke, T., Pereira, T. J., Hardy, S. M. and Bik, H. M. 2018. Nematode-associated microbial taxa do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats. Molecular Ecology 27:1930–1951. DOI: 10.1111/mec.14539. SchuelkeT. PereiraT. J. HardyS. M. BikH. M. 2018 Nematode-associated microbial taxa do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats Molecular Ecology 27 1930 1951 10.1111/mec.14539 Open DOISearch in Google Scholar

Shehzad, W., Riaz, T., Nawaz, M. A., Miquel, C., Poillot, C., Shah, S. A., Pompanon, F., Coissac, E. and Taberlet, P. 2012. Carnivore diet analysis based on next-generation sequencing: Application to the leopard cat (Prionailurus bengalensis) in Pakistan. Molecular Ecology 21:1951–1965. DOI: 10.1111/j.1365-294X.2011.05424.x. ShehzadW. RiazT. NawazM. A. MiquelC. PoillotC. ShahS. A. PompanonF. CoissacE. TaberletP. 2012 Carnivore diet analysis based on next-generation sequencing: Application to the leopard cat (Prionailurus bengalensis) in Pakistan Molecular Ecology 21 1951 1965 10.1111/j.1365-294X.2011.05424.x Open DOISearch in Google Scholar

Small, R. W. 1979. The effects of predatory nematodes on populations of plant-parasitic nematodes in pots. Nematologica 25:94–103. DOI: 10.1163/187529279x00424. SmallR. W. 1979 The effects of predatory nematodes on populations of plant-parasitic nematodes in pots Nematologica 25 94 103 10.1163/187529279x00424 Open DOISearch in Google Scholar

Small, R. W. 1987. A review of the prey of predatory soil nematodes. Pedobiologia 30:179–206. DOI: 10.1016/s0031-4056(23)00370-0. SmallR. W. 1987 A review of the prey of predatory soil nematodes Pedobiologia 30 179 206 10.1016/s0031-4056(23)00370-0 Open DOISearch in Google Scholar

Small, R. W. and Grootaert, P. 1983. Observations on the predation abilities of some soil-dwelling predatory nematodes. Nematologica 29:109–118. DOI: 10.1163/187529283x00230. SmallR. W. GrootaertP. 1983 Observations on the predation abilities of some soil-dwelling predatory nematodes Nematologica 29 109 118 10.1163/187529283x00230 Open DOISearch in Google Scholar

Soininen, E. M., Zinger, L., Gielly, L., Bellemain, E., Bråthen, K. A., Brochmann, C., Epp, L. S., Gussarova, G., Hassel, K., Henden, J., Killengreen, S. T., Rama, T., Stenoien, H. K., Yoccoz, N. G. and Ims, R. A. 2013. Shedding new light on the diet of Norwegian lemmings: DNA metabarcoding of stomach content. Polar Biology 36:1069–1076. DOI: 10.1007/s00300-013-1328-2. SoininenE. M. ZingerL. GiellyL. BellemainE. BråthenK. A. BrochmannC. EppL. S. GussarovaG. HasselK. HendenJ. KillengreenS. T. RamaT. StenoienH. K. YoccozN. G. ImsR. A. 2013 Shedding new light on the diet of Norwegian lemmings: DNA metabarcoding of stomach content Polar Biology 36 1069 1076 10.1007/s00300-013-1328-2 Open DOISearch in Google Scholar

Stoeck, T., Bass, D., Nebel, M., Christen, R., Jones, M. D. M., Breiner, H. W. and Richards, T. A. 2010. Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water. Molecular Ecology 19:21–31. DOI: 10.1111/j.1365-294X.2009.04480.x. StoeckT. BassD. NebelM. ChristenR. JonesM. D. M. BreinerH. W. RichardsT. A. 2010 Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water Molecular Ecology 19 21 31 10.1111/j.1365-294X.2009.04480.x Open DOISearch in Google Scholar

Tahseen, Q., Mohammad, A., Mustaqim, M., Ahlawat, S. and Bert, W. 2013. Descriptions of ten known species of the superfamily Mononchoidea (Mononchida: Nematoda) from North India with a detailed account on their variations. Zootaxa 3646:301–335. DOI: 10.11646/zootaxa.3646.4.1. TahseenQ. MohammadA. MustaqimM. AhlawatS. BertW. 2013 Descriptions of ten known species of the superfamily Mononchoidea (Mononchida: Nematoda) from North India with a detailed account on their variations Zootaxa 3646 301 335 10.11646/zootaxa.3646.4.1 Open DOISearch in Google Scholar

Valentini, A., Miquel, C., Nawaz, M.A., Bellemain, E., Coissac, E., Pompanon, F., Gielly, L., Cruaud, C., Nascetti, G., Wincker, P., Swenson, J. E. and Taberlet, P. 2009. New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: The trnL approach. Molecular Ecology Resources 9:51–60. DOI: 10.1111/j.1755-0998.2008.02352.x. ValentiniA. MiquelC. NawazM.A. BellemainE. CoissacE. PompanonF. GiellyL. CruaudC. NascettiG. WinckerP. SwensonJ. E. TaberletP. 2009 New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: The trnL approach Molecular Ecology Resources 9 51 60 10.1111/j.1755-0998.2008.02352.x Open DOISearch in Google Scholar

Vestheim, H. and Jarman, S. N. 2008. Blocking primers to enhance PCR amplification of rare sequences in mixed samples - A case study on prey DNA in Antarctic krill stomachs. Frontiers in Zoology 5. DOI: 10.1186/1742-9994-5-12. VestheimH. JarmanS. N. 2008 Blocking primers to enhance PCR amplification of rare sequences in mixed samples - A case study on prey DNA in Antarctic krill stomachs Frontiers in Zoology 5 10.1186/1742-9994-5-12 Open DOISearch in Google Scholar

Waeyenberge, L., de Sutter, N., Viaene, N. and Haegeman, A. 2019. New insights into nematode DNA metabarcoding as revealed by the characterization of artificial and spiked nematode communities. Diversity 11. DOI: 10.3390/d11040052. WaeyenbergeL. de SutterN. ViaeneN. HaegemanA. 2019 New insights into nematode DNA metabarcoding as revealed by the characterization of artificial and spiked nematode communities Diversity 11 10.3390/d11040052 Open DOISearch in Google Scholar

Whitehead, A. G. and Hemming, J. R. 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology 55:25–38. DOI: 10.1111/j.1744-7348.1965.tb07864.x. WhiteheadA. G. HemmingJ. R. 1965 A comparison of some quantitative methods of extracting small vermiform nematodes from soil Annals of Applied Biology 55 25 38 10.1111/j.1744-7348.1965.tb07864.x Open DOISearch in Google Scholar

Wood, F. H. 1973. Nematode feeding relationships feeding relationships of soil-dwelling nematodes. Soil Biology and Biochemistry 5:593–601. DOI: 10.1016/0038-0717(73)90049-7. WoodF. H. 1973 Nematode feeding relationships feeding relationships of soil-dwelling nematodes Soil Biology and Biochemistry 5 593 601 10.1016/0038-0717(73)90049-7 Open DOISearch in Google Scholar

Yeates, G. W. 1969. Predation by Mononchoides potohikus (Nematoda: Diplogasteridae) in laboratory culture. Nematologica 15:3–9. DOI: 10.1163/187529269x00029. YeatesG. W. 1969 Predation by Mononchoides potohikus (Nematoda: Diplogasteridae) in laboratory culture Nematologica 15 3 9 10.1163/187529269x00029 Open DOISearch in Google Scholar

Yeates, G. W. 1987. Nematode feeding and activity: the importance of development stages. Biol Fertil Soils 3:143–146. YeatesG. W. 1987 Nematode feeding and activity: the importance of development stages Biol Fertil Soils 3 143 146 Search in Google Scholar

Yeates, G. W., Bongers, T., De Goede, R. G. M., Freckman, D. W. and Georgieva, S. S. 1993. Feeding habits in soil nematode families and genera-An outline for soil ecologists. Journal of Nematology 25:315–331. YeatesG. W. BongersT. De GoedeR. G. M. FreckmanD. W. GeorgievaS. S. 1993 Feeding habits in soil nematode families and genera-An outline for soil ecologists Journal of Nematology 25 315 331 Search in Google Scholar

Yilmaz, P., Parfrey, L. W., Yarza, P., Gerken, J., Pruesse, E., Quast, C., Schweer, T., Peplies, J., Ludwig, W. and Glockner, F. O. 2014. The SILVA and “all-species living tree project (LTP)” taxonomic frameworks. Nucleic acids research 42:D643–D648 YilmazP. ParfreyL. W. YarzaP. GerkenJ. PruesseE. QuastC. SchweerT. PepliesJ. LudwigW. GlocknerF. O. 2014 The SILVA and “all-species living tree project (LTP)” taxonomic frameworks Nucleic acids research 42 D643 D648 Search in Google Scholar