Morphological, molecular, and histopathological studies on  from  and its role as an environmental biological indicator

Mohammed Albeshr; Rajwa Alsharief; Fatimah Al-Otibi; Esam M. Al-Shaebi; Osama B. Mohammed; Rewaida Abdel-Gaber

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Morphological, molecular, and histopathological studies on Hadjelia truncata from Columba livia domestica and its role as an environmental biological indicator

oraz

| 16 lis 2023

Journal of Nematology

Tom 55 (2023): Zeszyt 1 (February 2023)

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Article Category: Research Paper

Data publikacji: 16 lis 2023

Zakres stron: -

Otrzymano: 13 lip 2023

DOI: https://doi.org/10.2478/jofnem-2023-0050

Słowa kluczowe
Parasites, Identification tools, Negative impacts, Biomonitoring

© 2023 Mohammed Albeshr et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Photomicrographs for the Hadjelia truncata infecting the domestic pigeons. (A–F) Anterior extremity. (G–I) Middle part for female worms. (J–L) The posterior extremity of females. (M) Embryonated egg in feces. (N–Q) The posterior extremity of male worms. Note: L, lips; IL, interlabia, PA, papillae; CU, cuticle; PH, pharynx; MOE, muscular oesophagus; IN, intestine; GOE, glandular esophagus; Black arrow, constriction at the end of pharynx; EG, embryonated eggs; RT, rectum; IN, intestine; T, tail; AN, anal opening; White arrow, vaginal opening, UT, uterus; RSP, right spicules; LSP, left spicules; PCP, pre-cloacal papillae; POCP, post-cloacal papillae; CA, caudal alae; CO, cloacal opening.

A consensus phylogenetic tree constructed with neighbor joining (NJ) and maximum likelihood (ML) methods, showing phylogenetic relationships between Hadjelia truncata and related taxa in NCBI GenBank with Ascaris lumbricoides as an outgroup. The ML and NJ trees are inferred from the 18S rDNA sequences data generated from the H. truncata recovered from Columba livia domestica (OR122274 to OR122276 given in bold) and related taxa from GenBank. Numbers indicated at branch nodes are bootstrap values (ML/NJ). Only bootstraps > 60% are shown.

Photographs of the gizzards of the domestic pigeons. (A) Non-infected gizzard. (B) Enlargement of the infected gizzard. (C) Normal koilin layer. (D and E) Nematodes infection under the koilin layer of the gizzard.

(A–C) Histopathological sections from the gizzard’s wall infected with Hadjelia truncata, exhibiting the transverse sections of the nematodes (black arrows) with a large number of embryonated eggs (EG). The koilin layer is disrupted and fragmented with multiple clear spaces (asterisks).

Heavy metals in the gizzard of the domestic pigeons and its parasite

Heavy metals	Non-infected pigeon	Infected pigeon	Parasite
Fe	2.176 ± 0.02	0.741 ± 0.02 ^a	1.463 ± 0.02 ^ab
Cu	0.690 ± 0.01	0.120 ± 0.01 ^a	0.313 ± 0.01 ^ab
Zn	0.911 ± 0.02	0.409 ± 0.01 ^a	0.522 ± 0.02 ^ab
Cd	0.034 ± 0.001	0.010 ± 0.001 ^a	0.036 ± 0.001 ^ab
Cr	0.559 ± 0.01	0.267 ± 0.01 ^a	0.518 ± 0.02 ^ab
Co	0.166 ± 0.01	0.086 ± 0.001 ^a	0.114 ± 0.01 ^ab

Bioaccumulation of heavy metals concerning nematode parasite/pigeon model

Heavy metals	C_{[Hadjelia truncata]}/C_{[pigeon organ]}

	Liver	Muscles	Gizzard
Fe	1.431	1.675	1.974
Cu	2.371	2.445	2.608
Zn	2.063	1.611	1.276
Cd	3.272	2.250	3.600
Cr	1.519	5.630	1.940
Co	1.096	1.341	1.325

Morphological characteristics of male worms for Hadjelia truncata from the domestic pigeons

Source of Hadjelia truncata	Body		Esophagus		Distance of nerve ring from anterior end	Spicule length		Caudal papillae	Tail length

	Length	Width	Muscular length	Glandular length		Right	Left
Cram, 1927	5–7	–	–	–	–	0.220	1.6	Six pairs (4 pre- & 2 post-) of caudal papillae	–
Baer, 1954	8	0.285	0.033	–	–	0.225–0.300	1.320–1.600		–
Ibrahim et al., 1995	4–8 (6.4)	–	1.8–2.7 (2.2)		–	–	–		–
Junker & Boomker, 2007	7–8	0.145–0.160	0.042–0.044	1.750–1.927	0.208–0.212	0.254–0.271	1.346–1.434		–
Razmi et al., 2007	7–9	–	–	–	–	0.34	1.26		–
Sentíes-Cué et al., 2011	6.5–9	–	–	–	–	0.35	1.27		–
Naem et al., 2013	6.5–9	0.151	–	–	–	0.35	1.27		–
Nabavi et al., 2013	1–2	–	–	–	–	0.320–0.350	1.410–1.470		–
Al-Moussawi, 2015	7–8 (7.5)	0.204–0.258 (0.231)	0.374–0.386 (0.380)	1.620–1.680 (1.650)	0.198–0.211 (0.204)	0.240–0.270 (0.255)	1.512–1.620 (1.566)		0.127–0.138 (0.132)
Oryan et al., 2016	7.5	–	–	–	–	–	–		–
Jameel et al., 2016	6.5–9	–	–	–	–	–	–		–
Khordadmehr et al., 2018	7–11	–	–	–	–	–	–		–
Present study, 2023	6.201–8.023 (7.270)	0.191–0.219 (0.204)	0.353–0.376 (0.367)	1.599–1.758 (1.623)	0.184–0.201 (0.197)	0.270–0.320 (0.284)	1.332–1.511 (1.416)		0.118–0.127 (0.122

Morphological characteristics of female worms for Hadjelia truncata from the domestic pigeons

Source of Hadjelia truncata	Body		Esophagus		Distance from anterior end		Eggs			Tail length

	Length	Width	Muscular length	Glandular length	Nerve ring	Vulva opening	Length	Width	Status
Cram, 1927	10–16	0.300	–	–	–	2.6–16	0.027	–	Numerous embryonated eggs covered with thick-shelled	–
Baer, 1954	8–16	0.143–0.285	–	–	–	1.640–3.000	–	–		–
Ibrahim et al., 1995	17–22 (20)	–	2.0–2.4 (2.4)		–	1.5–2.5 (2)	0.050	0.030		–
Junker & Boomker, 2007	10–11	0.140–0.217	0.005–0.007	1.948–2.076	0.159–0.185	1.691–2.238	0.050–0.053	0.032–0.035		0.121–0.138
Razmi et al., 2007	13–17	–	–	–	–	–	–	–		–
Al-Moussawi, 2008	14–15.22 (14.62)	0.168–0.199 (0.183)	0.512–0.57 (0.54)	0.589–0.622 (0.605)	0.003–0.024 (0.015)	–	–	–		0.113–0.150 (0.132)
Mohammad & Al-Moussawi, 2011	11.10–14.88 (13.15)	0.18–0.28 (0.23)	0.450–0.566 (0.461)	1.47–3.32 (2.435)	–	1.575–2.730 (2.281)	0.033–0.057 (0.044)	0.015–0.041 (0.034)		–
Sentíes-Cué et al., 2011	12–16.5	–	–	–	–	–	–	–		–
Naem et al., 2013	12	16.5	0.229	–	–	2.155	–	–		–
Nabavi et al., 2013	3–5	–	–	–	–		0.054–0.059	0.030–0.032
Al-Moussawi & Jassim, 2015	23.226–26.156 (24.594)	0.231–0.312 (0.2782)	3.510–3.666 (3.588)	–	0.260–0.312 (0.291)	3.276–3.413 (3.364)	0.052–0.104 (0.078)	0.021–0.312 (0.026)		0.104–0.234 (0.148)
Al-Moussawi, 2015	8–17 (12)	0.147–0.270 (0.224)	2.750–3.648 (3.379)	2.002–2.835 (2.215)	0.178–0.206 (0.192)	1.501–3.022 (2.108)	0.046–0.048 (0.047)	0.024–0.0408 (0.047)		0.145–0.230 (0.196)
Oryan et al., 2016	19.8	–	–	–	–	–	0.045	0.031		–
Jameel et al., 2016	12–16.5	–	–	–	–	–	–	–		–
Khordadmehr et al., 2018	15–20	–	–	–	–	–	0.043–0.045	0.020–0.030		–
Present study, 2023	12.270–18.583 (17.792)	0.256–0.314 (0.287)	3.515–3.759 (3.601)	2.160–2.211 (2.181)	0.198–0.225 (0.211)	3.011–3.054 (3.027)	0.046–0.051 (0.048)	0.029–0.033 (0.031)		0.118–0.132 (0.124)

Heavy metals in the liver of the domestic pigeons and its parasite

Heavy metals	Non-infected pigeon	Infected pigeon	Parasite
Fe	5.907 ± 0.02	1.022 ± 0.02 ^a	1.463 ± 0.02 ^ab
Cu	0.608 ± 0.02	0.132 ± 0.01 ^a	0.313 ± 0.00 ^ab
Zn	0.935 ± 0.02	0.253 ± 0.02 ^a	0.522 ± 0.02 ^ab
Cd	0.046 ± 0.001	0.011 ± 0.001 ^a	0.036 ± 0.001 ^ab
Cr	0.617 ± 0.02	0.341 ± 0.01 ^a	0.518 ± 0.02 ^ab
Co	0.212 ± 0.01	0.104 ± 0.01 ^a	0.114 ± 0.01 ^ab

Heavy metals in the muscle of the domestic pigeons and its parasite

Heavy metals	Non-infected pigeon	Infected pigeon	Parasite
Fe	2.222 ± 0.02	0.873 ± 0.02 ^a	1.463 ± 0.02 ^ab
Cu	0.582 ± 0.02	0.128 ± 0.01 ^a	0.313 ± 0.01 ^ab
Zn	0.974 ± 0.02	0.324 ± 0.02 ^a	0.522 ± 0.02 ^ab
Cd	0.050 ± 0.001	0.016 ± 0.001 ^a	0.036 ± 0.001 ^ab
Cr	0.592 ± 0.02	0.092 ± 0.001 ^a	0.518 ± 0.02 ^ab
Co	0.473 ± 0.01	0.085 ± 0.01 ^a	0.114 ± 0.01 ^ab

eISSN:: 2640-396X
Język:: Angielski

Częstotliwość wydawania:: Volume Open
Dziedziny czasopisma:: Life Sciences, other

Kanał RSS czasopisma

Morphological, molecular, and histopathological studies on Hadjelia truncata from Columba livia domestica and its role as an environmental biological indicator

Article Category: Research Paper

Data publikacji: 16 lis 2023

Zakres stron: -

Otrzymano: 13 lip 2023

DOI: https://doi.org/10.2478/jofnem-2023-0050

Słowa kluczowe
Parasites, Identification tools, Negative impacts, Biomonitoring

© 2023 Mohammed Albeshr et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Figure 5:

Heavy metals in the gizzard of the domestic pigeons and its parasite

Bioaccumulation of heavy metals concerning nematode parasite/pigeon model

Morphological characteristics of male worms for Hadjelia truncata from the domestic pigeons

Morphological characteristics of female worms for Hadjelia truncata from the domestic pigeons

Heavy metals in the liver of the domestic pigeons and its parasite

Heavy metals in the muscle of the domestic pigeons and its parasite

Morphological, molecular, and histopathological studies on Hadjelia truncata from Columba livia domestica and its role as an environmental biological indicator

Article Category: Research Paper

Data publikacji: 16 lis 2023

Zakres stron: -

Otrzymano: 13 lip 2023

DOI: https://doi.org/10.2478/jofnem-2023-0050

Słowa kluczoweParasites, Identification tools, Negative impacts, Biomonitoring

© 2023 Mohammed Albeshr et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Figure 5:

Heavy metals in the gizzard of the domestic pigeons and its parasite

Bioaccumulation of heavy metals concerning nematode parasite/pigeon model

Morphological characteristics of male worms for Hadjelia truncata from the domestic pigeons

Morphological characteristics of female worms for Hadjelia truncata from the domestic pigeons

Heavy metals in the liver of the domestic pigeons and its parasite

Heavy metals in the muscle of the domestic pigeons and its parasite

Słowa kluczowe
Parasites, Identification tools, Negative impacts, Biomonitoring