First Reports and Morphological and Molecular Characterization of Pratylenchus delattrei and Quinisulcius capitatus Associated with Chickpea in Ethiopia
, , , , , oraz | 11 cze 2023
O artykule
Article Category: Research Paper
Data publikacji: 11 cze 2023
Zakres stron: -
Otrzymano: 27 sty 2023
DOI: https://doi.org/10.2478/jofnem-2023-0027
Słowa kluczowe
© 2023 Habtamu Kefelegn et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
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Figure 4:
Comparison of important morphological character and morphometrics of the Ethiopian Quinisulcius capitatus, found from chickpea in Ethiopia, with original description from USA (Allen, 1955), and two other Q. capitatus from Ethiopia and Canada. All measurements are in μm and in the form: mean ± s.d. (range).
| n | 10 | 13 | 10 | 20 |
| L | 699 ± 11.6 (667 – 707) | 630 – 850 | 630 – 790 | 810.3 ± 44.6 (744.0 – 911.0) |
| a | 31.2 ± 1.3 (29.7 – 34.4) | 30 – 38 | 30.9 – 38.6 | 41.4 ± 1.8 (38.6 – 43.7) |
| b | 5.4 ± 0.2 (5.1 – 5.7) | 5.0 – 5.8 | – | 5.5 ± 0.3 (5.0 – 6.3) |
| c | 14.7 ± 0.7 (14.1 – 16.3) | 12 – 17 | 15.3 – 17.6 | 22.4 ± 1.1 (19.9 – 23.8) |
| c’ | 1.9 ± 0.2 (1.5 – 2.1) | – | – | 2.6 ± 0.2 (2.2 – 3.2) |
| V | 56.6 ± 2.2 (52.7 – 59.0) | 51 – 58 | 54.7 – 63.6 | 57.4 ± 1.5 (53.4 – 59.8) |
| Stylet length | 18.8 ± 0.6 (17.8 – 19.7) | 16 – 18 | 15 – 18 | 18.3 ± 1.0 (15.5 – 20.4) |
| Lip height | 4.0 ± 0.5 (3.2 – 4.5) | – | – | 4.0 ± 0.2 (3.7 – 4.4) |
| Lip width | 7.6 ± 0.5 (7.0 – 8.3) | – | – | 7.6 ± 0.4 (6.9 – 8.3) |
| Median bulb length | 16.1 ± 0.3 (15.7 – 16.6) | – | – | 14.0 ± 1.6 (11.3 – 16.9) |
| Median bulb width | 13.1 ± 0.6 (12.2 – 13.9) | – | – | 10.4 ± 1.4 8.4 – 14.2 |
| Pharyngeal length | 129 ± 5.1 (123 – 138) | – | – | 147.8 ± 5.8 (140.2 – 159.0) |
| SE pore from anterior end | 121 ± 3.3 (113 – 123) | – | 121 | 128.6 ± 5.3 (121.0 – 139.0) |
| Midbody diameter | 22.4 ± 1.0 (20.5 – 23.5) | 21 – 27 | – | – |
| Tail length | 47.8 ± 3.0 (41.0 – 50.0) | – | – | 35.8 ± 2.4 (31.3 – 40.4) |
| Anal body diameter | 25.3 ± 3.2 (23.0 – 34.2) | – | – | – |
| Phasmid position | Middle of tail | Middle of tail | – | Middle of tail |
Pratylenchus delattrei and Quinisulcius capitatus recovered from chickpea roots and soil, districts, sampling locality, laboratory codes, altitude, GPS coordinators and GenBank accession numbers.
| Minjar | Kitecha | Mki-5 | 1600-1800 | 8°52′58.16″N | 39°29′46.01″E | OP646170 | - | - | |
| Mki-8 | OP646169 | - | - | ||||||
| Mki-12 | - | - | OP730534 | ||||||
| Adea’a | Gollodhertu | AG-1 | 1800-1900 | 8°38′55.54″N | 38°55′0.94″E | - | OP646171 | - | |
| AG-2 | OP646168 | OP646172 | - | ||||||
| AG-3 | OP646167 | - | OP730535 | ||||||
| Mesekan | Jolle-2 | JO2-3 | 1900-1950 | 8°11′49.79″N | 38°27′51.24″E | OP626319 | OP646173 | OP627909 | |
| JO2-5 | OP626320 | OP646174 | OP627910 | ||||||
| JO2-7 | OP626321 | OP646175 | OP627911 | ||||||
Comparison of morphometrics of the Ethiopian Pratylenchus delattrei from chickpea in Ethiopia with the original description from Madagascar (Luc, 1958), and two other P. delattrei populations from Cape Verde and Iran. All measurements are in μm and in the form: mean ± s.d. (range).
| n | 10 | 13 | 20 | 7 | 12 |
| L | 475 ± 47.1 (410 – 560) | 390 – 470 | 532 ± 33 (498 – 586) | 543 ± 55 (467 – 616) | 508 ± 49.2 (434 – 576) |
| a | 25.2 ± 2.2 (22.8 – 29) | 20.4 – 25.8 | 26.6 ± 2.2 (22.1 – 31.3) | 23.8 ± 2.1 (21.2 – 26.9) | 22.6 ± 1.0 (21.1 – 25) |
| b | 5.6 ± 0.6 (4.8 – 6.6) | 3.7 – 4.8 | 6.6 ± 0.5 (6.1 – 7.7) | 6.1 ± 0.7 (5.0 – 7.2) | 5.9 ± 0.6 (5.2 – 6.9) |
| b’ | 4.7 ± 0.5 (4.1 – 5.6) | – | 4.5 ± 0.4 (4.0 – 5.3) | 4.3 ± 0.2 (4.0 – 4.6) | 4.1 ± 0.4 (3.6 – 4.9) |
| c | 14.6 ± 1.9 (13.7 – 20.3) | 18 – 22.3 | 21.9 ± 2.1 (18.5 – 25.1) | 20 ± 2 (18.1 – 23.1) | 19.7 ± 2.6 (16.7 – 24.1) |
| c’ | 2.3 ± 0.2 (2.0 – 2.8) | – | 2.2 ± 0.2 (1.9 – 2.8) | 2.2 ± 0.3 (1.9 – 2.6) | 1.9 ± 0.2 (1.6 – 2.2) |
| V | 72.2 ± 7.6 (62.4 – 86.9) | 73 – 81 | 76 ± 1 (75 – 78) | 75.1 ± 1.9 (71.4 – 77.1) | 75.9 ± 1.3 (74 – 78.7) |
| Stylet length | 16.7 ± 0.7 (15.8 – 17.8) | 16.5 – 18.0 | 16.4 ± 0.4 (15.4 – 16.9) | 16.3 ± 0.8 (15 – 17) | 16.0 ± 0.6 (15 – 17) |
| Dorsal gland opening from stylet base | 3.0 ± 0.5 (2.3 – 3.5) | – | 2.9 ± 0.3 (2.4 – 3.1) | – | – |
| O | 17.7 ± 2.9 (13.6 – 22.2) | – | 17.4 ± 1.7 (14.3 – 21.3) | – | – |
| Pharynx length | 85 ± 1.0 (82.5 – 85.9) | – | 80.1 ± 3.2 (73.1 – 84.2) | 89 ± 10.4 (76 – 105) | 87 ± 4 (82 – 94) |
| Pharyngeal overlap | – | – | 39.0 ± 7.2 (29.8 – 49.0) | – | |
| Anterior end to end of pharyngeal gland lobe | 101 ± 1.4 (98 – 102) | – | – | 125 ± 7.3 (116 – 135) | 123 ± 8.3 (113 – 138) |
| Maximal body diameter | 22.7 ± 0.5 (21.6 – 23.1) | – | 20.2 ± 2.2 (16.8 – 23.7) | 23 ± 3.3 (20 – 29) | 22.5 ± 2.1 (19 – 26) |
| Anal body diameter | 13.0 ± 1.2 (10.9 – 14.1) | – | 11.2 ± 1.2 (8.9 – 13.2) | 12.4 ± 1.5 (11 – 14) | 13 ± 0.9 (12 – 14) |
| Tail length | 29.0 ± 0.9 (27.5 – 30.1) | – | 24.8 ± 2.3 (21.0 – 27.1) | 26.6 ± 2.3 (23 – 29) | 24.8 ± 2.3 (23 – 29) |
| Tail annuli | 20 ± 2 (18 – 23) | – | 19 ± 2 (16 – 24) | 20 ± 2.1 (18 – 23) | 19 ± 1.5 (17 – 21) |
| Phasmid to terminus | 14.1 ± 0.4 (13.5 – 14.5) | – | 10.6 ± 2.7 (6.0 – 14.9) | – | – |