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Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis)

Amanda G. Gaudin
Amanda G. Gaudin
, 
Martin J. Wubben
Martin J. Wubben
, 
Jack C. McCarty
Jack C. McCarty
 oraz 
Johnie N. Jenkins
Johnie N. Jenkins
   | 23 cze 2023
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Article Category: Research Paper
Data publikacji: 23 cze 2023
Zakres stron: -
Otrzymano: 26 sie 2022
DOI: https://doi.org/10.2478/jofnem-2023-0021
Słowa kluczowe
© 2023 Amanda G. Gaudin et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) in comparison to a greenhouse-maintained RKN race 3 population. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Eggs were used as inoculum and virulence was measured as (A) RF, (B) log10 eggs g−1 root, and (C) gall score index (1–10 scale). Tukey-Kramer multiple comparison was used to determine significant differences between means at α = 0.05 as denoted by different letters (n = 6).
Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) in comparison to a greenhouse-maintained RKN race 3 population. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Eggs were used as inoculum and virulence was measured as (A) RF, (B) log10 eggs g−1 root, and (C) gall score index (1–10 scale). Tukey-Kramer multiple comparison was used to determine significant differences between means at α = 0.05 as denoted by different letters (n = 6).

Figure 2:

Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) using second-stage juveniles as inoculum. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Virulence was measured as (A) RF ± SE, (B) log10 eggs g−1 root ± SE, and (C) gall score index ± SE (1–10 scale) of six replicates.
Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) using second-stage juveniles as inoculum. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Virulence was measured as (A) RF ± SE, (B) log10 eggs g−1 root ± SE, and (C) gall score index ± SE (1–10 scale) of six replicates.

Figure 3:

Time-point dependent reproduction of the Meloidogyne enterolobii isolate NC.1 on cotton germplasm lines M240 RNR and MRk-Rn-1 in comparison to the susceptible cultivar SureGrow747 (SG747). Total eggs were extracted at 35-, 42-, 49-, and 56-days post inoculation (DPI). Isolate NC.1 reproduction is presented as (A) RF (total eggs extracted/initial inoculum) and (B) log10 eggs g−1 root. Significant differences between means at α = 0.05 noted by different letters (n = 6).
Time-point dependent reproduction of the Meloidogyne enterolobii isolate NC.1 on cotton germplasm lines M240 RNR and MRk-Rn-1 in comparison to the susceptible cultivar SureGrow747 (SG747). Total eggs were extracted at 35-, 42-, 49-, and 56-days post inoculation (DPI). Isolate NC.1 reproduction is presented as (A) RF (total eggs extracted/initial inoculum) and (B) log10 eggs g−1 root. Significant differences between means at α = 0.05 noted by different letters (n = 6).

Figure 4:

Time-course of Meloidogyne enterolobii isolate NC.1 infection of SureGrow747 and MRk-Rn-1. Infected roots were collected at 4-, 8-, 12-, 16-, 20-, and 24-days after inoculation (DAI) and stained with acid fuchsin.
Time-course of Meloidogyne enterolobii isolate NC.1 infection of SureGrow747 and MRk-Rn-1. Infected roots were collected at 4-, 8-, 12-, 16-, 20-, and 24-days after inoculation (DAI) and stained with acid fuchsin.

Effect of Meloidogyne enterolobii infection on cotton growth under growth chamber conditions. Presented are mean ± SE after 6 weeks of growth under uninoculated (control) or inoculated conditions (n = 5).

Control Inoculated
Stem Height (mm) DPL61 124.1 ± 3.2 79.3 ± 3.7**
M240 RNR 147.8 ± 4.6 133.8 ± 22.8
SG747 143.5 ± 2.8 89.6 ± 2.1**
M713 Ren1 144.0 ± 17.0 131.0 ± 12.8
MRk-Rn-1 157.6 ± 10.5 111.6 ± 5.4**
Shoot Fresh Weight (g) DPL61 2.49 ± 0.13 1.94 ± 0.23
M240 RNR 2.75 ± 0.23 2.01 ± 0.27
SG747 3.23 ± 0.45 1.48 ± 0.19**
M713 Ren1 2.58 ± 0.22 3.39 ± 0.42
MRk-Rn-1 2.99 ± 0.74 1.83 ± 0.11
Leaf Number DPL61 3.7 ± 0.1 3.8 ± 0.4
M240 RNR 3.8 ± 0.1 3.5 ± 0.5
SG747 3.5 ± 0.2 2.4 ± 0.3**
M713 Ren1 3.9 ± 0.3 3.8 ± 0.3
MRk-Rn-1 4.1 ± 0.3 3.1 ± 0.2**
Stem Height/Node Number DPL61 28.9 ± 0.5 21.1 ± 1.8**
M240 RNR 33.2 ± 1.9 33.2 ± 1.5
SG747 40.0 ± 2.3 26.5 ± 0.9**
M713 Ren1 31.7 ± 4.7 27.6 ± 2.2
MRk-Rn-1 40.5 ± 2.3 29.5 ± 1.1**
eISSN:
2640-396X
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
Life Sciences, other
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