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Morphological and Molecular Characterization of Filenchus multistriatus n. sp. (Tylenchomorpha: Tylenchidae) and Data on a Known Species of the Genus from Bushehr Province, Southern Iran

Somayeh Monemi
Somayeh Monemi
, 
Mohammad Reza Atighi
Mohammad Reza Atighi
, 
Joaquín Abolafia
Joaquín Abolafia
, 
Pablo Castillo
Pablo Castillo
 oraz 
Majid Pedram
Majid Pedram
   | 17 maj 2023
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Article Category: Research paper
Data publikacji: 17 maj 2023
Zakres stron: -
Otrzymano: 04 sty 2023
DOI: https://doi.org/10.2478/jofnem-2023-0008
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© 2023 Somayeh Monemi et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Line drawings of Filenchus multistriatus n. sp. (Female). (A) Entire body. (B) Tail. (C) Pharynx. (D) Lateral view of lateral field. (E) Lateral field.
Line drawings of Filenchus multistriatus n. sp. (Female). (A) Entire body. (B) Tail. (C) Pharynx. (D) Lateral view of lateral field. (E) Lateral field.

Figure 2:

Light microphotographs of Filenchus multistriatus n. sp. (Female). (A&B) Anterior body region (arrows pointing to the excretory pore). (C) Entire body. (D) Reproductive system. (E) Tail. (F&G) Lateral field in two paratypes in lateral view.
Light microphotographs of Filenchus multistriatus n. sp. (Female). (A&B) Anterior body region (arrows pointing to the excretory pore). (C) Entire body. (D) Reproductive system. (E) Tail. (F&G) Lateral field in two paratypes in lateral view.

Figure 3:

Scanning electron microphotographs of Filenchus multistriatus n. sp. (Female). (A-C) Lip region in ventral and subfrontal views, respectively (arrows pointing to the amphidial openings). (D) Anterior body region. (E&F) Lip region in frontal view (arrows pointing to the amphidial openings). (G, I, J) Lateral field. (H) Tail (arrow pointing to the anus). (K) Vulva.
Scanning electron microphotographs of Filenchus multistriatus n. sp. (Female). (A-C) Lip region in ventral and subfrontal views, respectively (arrows pointing to the amphidial openings). (D) Anterior body region. (E&F) Lip region in frontal view (arrows pointing to the amphidial openings). (G, I, J) Lateral field. (H) Tail (arrow pointing to the anus). (K) Vulva.

Figure 4:

Light microphotographs of Iranian population of F. sandneri Wasilewska, 1965 from Bushehr province (Female). (A) Anterior body region (arrows pointing to the excretory pore). (B) Reproductive system. (C) Lip region and stylet. (D) Lateral field. (E) Tail. (F) Entire body.
Light microphotographs of Iranian population of F. sandneri Wasilewska, 1965 from Bushehr province (Female). (A) Anterior body region (arrows pointing to the excretory pore). (B) Reproductive system. (C) Lip region and stylet. (D) Lateral field. (E) Tail. (F) Entire body.

Figure 5:

Scanning electron microphotographs of Iranian population of F. sandneri Wasilewska, 1965 from Bushehr province (Female). (A-C) Lip region in ventral, subfrontal and frontal views, respectively (arrows pointing to the amphidial openings). (D) Excretory pore. (E) Lateral field. (F) Tail (upper and lower arrows pointing to the vulva and anus, respectively). (G,H) Lateral fields. (I) Vulva. (J) Anus.
Scanning electron microphotographs of Iranian population of F. sandneri Wasilewska, 1965 from Bushehr province (Female). (A-C) Lip region in ventral, subfrontal and frontal views, respectively (arrows pointing to the amphidial openings). (D) Excretory pore. (E) Lateral field. (F) Tail (upper and lower arrows pointing to the vulva and anus, respectively). (G,H) Lateral fields. (I) Vulva. (J) Anus.

Figure 6:

Bayesian 50% majority rule consensus tree inferred from SSU rDNA of Filenchus multistriatus n. sp. under the GTR + G + I model (-ln L = 20913.9590; freq A = 0.2301; freqC = 0.2419; freqG = 0.3006; freq T = 0.2273; R(a) = 0.8974; R(b) = 2.1547; R(c) = 0.9066; R(d) = 1.0666; R(e) = 4.2155; R(f) = 1.0000; Pinva = 0.2800; Shape = 0.4692). Bayesian posterior probability values are given for appropriate clades. The newly generated sequence of the new species is in bold font.
Bayesian 50% majority rule consensus tree inferred from SSU rDNA of Filenchus multistriatus n. sp. under the GTR + G + I model (-ln L = 20913.9590; freq A = 0.2301; freqC = 0.2419; freqG = 0.3006; freq T = 0.2273; R(a) = 0.8974; R(b) = 2.1547; R(c) = 0.9066; R(d) = 1.0666; R(e) = 4.2155; R(f) = 1.0000; Pinva = 0.2800; Shape = 0.4692). Bayesian posterior probability values are given for appropriate clades. The newly generated sequence of the new species is in bold font.

Figure 7:

Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 sequences of Filenchus multistriatus n. sp. under the GTR+G+I model (-ln L = 22199.4473; freq A = 0.1507; freqC = 0.2476; freqG = 0.3242; freq T = 0.2775; R(a) = 0.8948; R(b) = 0.8948; R(c) = 1.0152; R(d) = 0.7990; R(e) = 3.4497; R(f) = 1.0000; Pinva = 0.1528; Shape = 0.7240). Bayesian posterior probability values are given for appropriate clades. The newly generated sequences of the new species are in bold font.
Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 sequences of Filenchus multistriatus n. sp. under the GTR+G+I model (-ln L = 22199.4473; freq A = 0.1507; freqC = 0.2476; freqG = 0.3242; freq T = 0.2775; R(a) = 0.8948; R(b) = 0.8948; R(c) = 1.0152; R(d) = 0.7990; R(e) = 3.4497; R(f) = 1.0000; Pinva = 0.1528; Shape = 0.7240). Bayesian posterior probability values are given for appropriate clades. The newly generated sequences of the new species are in bold font.

Morphometrics of Filenchus multistriatus n. sp. and F. sandneri Wasilewska, 1965 from Bushehr province, Iran. All measurements are in μm and in the form: mean ± SD (range).

F. multistriatus n. sp. F. sandneri
Character Holotype Paratypes Bushehr population
Sex Female Female Female
n 1 11 9
L 567 537.3±33.0 (500-590) 400.1±48.0 (331-454)
L′ 497 464.4±33.1 (423-515) 352.8±46.0 (288-406)
a 40.5 35.0±3.5 (29.3-40.5) 31.1±4.0 (25.5-37.6)
b 5.3 5.5±0.3 (5.0-6.0) 5.1±0.5 (4.2-5.9)
c 8.1 7.4±0.5 (6.4-8.4) 8.4±0.8 (7.3-9.5)
c′ 7.0 7.2±0.9 (5.8-8.3) 5.7±0.3 (5.3-6.5)
V 70.5 69.6±3.0 (63.2-73.8) 73.1±2.5 (68.1-75.8)
V′ 80.5 81.5±1.9 (77.8-84.9) 83.7±1.8 (81.4-86.2)
Lip region height 1.8 1.8±0.1 (1.7-1.9) 2.1±0.3 (1.5-2.4)
Lip region width 0.7 6.8±0.3 (6-7) 5.0±0.2 (4.5-5.3)
Stylet 6.6 7.1±0.4 (6.5-7.6) 8.0±0.5 (7.5-8.6)
Conus 2.1 2.5±0.4 (2-3) 2.8±0.2 (2.6-3.0)
m 31.8 35.5±3.5 (30.8-39.5) 35.4±1.8 (32.5-38.5)
DGO 0.7 0.7±0.1 (0.5-0.8) 2.7±0.2 (2.5-3.0)
Excretory pore 81 75.4±5.5 (67-81) 57.7±5.5 (50-64)
Median bulb 47 38.2±4.4 (35-47) --
MB 43.9 38.7±3.9 (34.7-48.5) 35.8±2.8 (30.5-41.3)
Pharynx 107 98.6±3.8 (94-107) 78.1±5.9 (71-90)
Body width (BW) 14 15.5±2.0 (14-20) 12.9±0.9 (12-14)
Anterior end to vulva 400 374±35 (316-427) 292.8±40.3 (236-343)
Vulva - anus 97 85±11 (67-105) 57.0±8.4 (40-70)
Anal body width 10 10.3±1.0 (9-12) 8.3±0.5 (8-9)
PUS 12 12.2±0.7 (11-13) 8.3±0.4 (7.8-8.8)
PUS/BW 0.9 0.8±0.1 (0.6-0.9) 8.3±0.4 (7.8-8.8)
Tail 70 72.9±3.1 (70-78) 47.3±3.1 (43-52)
Tail/V-A 0.7 0.9±0.1 (0.7-1.2) 0.8±0.1 (0.6-1.2)
eISSN:
2640-396X
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
Life Sciences, other
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