<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Longidorid nematodes comprise more than 500 species, and <italic>Longidorus</italic> and <italic>Xiphinema</italic> are the most diversified, prevalent, and cosmopolitan genera within plant-parasitic nematodes. The genus <italic>Longidorus</italic> comprise a group of species, some of which are vectors of plant viruses. New sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, northern Greece, and one nematode species of <italic>Longidorus</italic> (<italic>L. leptocephalus</italic>) was recovered. Nematodes were extracted from soil samples by modified sieving and a decanting method. Extracted specimens were processed using glycerol, mounted on permanent slides, and subsequently identified morphologically. Nematode DNA was extracted from individual, live specimens, and PCR (Polymerase Chain Reaction) assays were performed for D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, and partial mitochondrial <italic>COI</italic> regions. Morphology and morphometric data obtained from this population were consistent with the original description and reported populations of <italic>L. leptocephalus</italic>. To our knowledge, this is the first report of <italic>L. leptocephalus</italic> in Greece and the second in the Mediterranean Basin after the record of the species from Slovenia, extending the geographical distribution of this species in Europe.</p></abstract>

Longidorid nematodes comprise more than 500 species, and Longidorus and Xiphinema are the most diversified, prevalent, and cosmopolitan genera within plant-parasitic nematodes. The genus Longidorus comprise a group of species, some of which are vectors of plant viruses. New sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, northern Greece, and one nematode species of Longidorus (L. leptocephalus) was recovered. Nematodes were extracted from soil samples by modified sieving and a decanting method. Extracted specimens were processed using glycerol, mounted on permanent slides, and subsequently identified morphologically. Nematode DNA was extracted from individual, live specimens, and PCR (Polymerase Chain Reaction) assays were performed for D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, and partial mitochondrial COI regions. Morphology and morphometric data obtained from this population were consistent with the original description and reported populations of L. leptocephalus. To our knowledge, this is the first report of L. leptocephalus in Greece and the second in the Mediterranean Basin after the record of the species from Slovenia, extending the geographical distribution of this species in Europe.

First report of <italic>Longidorus leptocephalus</italic> <xref ref-type="bibr" rid="j_jofnem-2022-0027_ref_029">Hooper, 1961</xref> (Nematoda: Longidoridae) from Greece

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

First report of   (Nematoda: Longidoridae) from Greece

Longidorid nematodes comprise more than 500 species, and Longidorus and Xiphinema are the most diversified, prevalent, and cosmopolitan genera within...

First report of Longidorus leptocephalus Hooper, 1961 (Nematoda: Longidoridae) from Greece

Article Category: Research Paper

Data publikacji: 29 lip 2022

Zakres stron: -

Otrzymano: 22 kwi 2022

DOI: https://doi.org/10.2478/jofnem-2022-0027

Słowa kluczowe
cytochrome oxidase c subunit 1, D2-D3 of 28S rDNA, description, ITS1 rDNA, needle nematodes, taxonomy

© 2022 Clavero-Camacho et al. published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

First report of Longidorus leptocephalus Hooper, 1961 (Nematoda: Longidoridae) from Greece

Article Category: Research Paper

Data publikacji: 29 lip 2022

Zakres stron: -

Otrzymano: 22 kwi 2022

DOI: https://doi.org/10.2478/jofnem-2022-0027

Słowa kluczowecytochrome oxidase c subunit 1, D2-D3 of 28S rDNA, description, ITS1 rDNA, needle nematodes, taxonomy

© 2022 Clavero-Camacho et al. published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Słowa kluczowe
cytochrome oxidase c subunit 1, D2-D3 of 28S rDNA, description, ITS1 rDNA, needle nematodes, taxonomy