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Pollen Count Dynamics in Rapeseed Stamens in Early Spring

Zhijun Wei
Zhijun Wei
, 
Ran Liu
Ran Liu
, 
Guiling Ding
Guiling Ding
, 
Yusuo Jiang
Yusuo Jiang
 oraz 
Jiaxing Huang
Jiaxing Huang
   | 22 gru 2023
Journal of Apicultural Science's Cover Image
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Article Category: Original Article
Data publikacji: 22 gru 2023
Zakres stron: 103 - 114
Otrzymano: 17 kwi 2023
Przyjęty: 09 paź 2023
DOI: https://doi.org/10.2478/jas-2023-0008
Słowa kluczowe
© 2023 Zhijun Wei et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1.

The number of pollen grains in 5 μl of a suspension solution pipetted from a 1-cm depth below the liquid surface was counted by hemocytometry after different standing times in four suspension solutions (A. 10% sucrose + 0.05% agar solution, B. 20% sodium hexametaphosphate solution, C. 5% sucrose + 0.1% agar solution and D. distilled water). Data are presented as the means ± SEs.
The number of pollen grains in 5 μl of a suspension solution pipetted from a 1-cm depth below the liquid surface was counted by hemocytometry after different standing times in four suspension solutions (A. 10% sucrose + 0.05% agar solution, B. 20% sodium hexametaphosphate solution, C. 5% sucrose + 0.1% agar solution and D. distilled water). Data are presented as the means ± SEs.

Fig. 2.

The number of pollen grains in 5 μl of a suspension solution pipetted from different depths below the liquid surface was counted by hemocytometry in four suspension solutions (A. 10% sucrose + 0.05% agar solution, B. 20% sodium hexametaphosphate solution, C. 5% sucrose + 0.1% agar solution and D. distilled water). Data are presented as the means ± SEs.
The number of pollen grains in 5 μl of a suspension solution pipetted from different depths below the liquid surface was counted by hemocytometry in four suspension solutions (A. 10% sucrose + 0.05% agar solution, B. 20% sodium hexametaphosphate solution, C. 5% sucrose + 0.1% agar solution and D. distilled water). Data are presented as the means ± SEs.

Fig. 3.

The linear relationship between the number of pollen grains in 5 μl of a 5% sucrose + 0.1% agar suspension solution assessed by hemocytometry under microscopy and the absorbance measured by light spectroscopy at a wavelength of 425 nm.
The linear relationship between the number of pollen grains in 5 μl of a 5% sucrose + 0.1% agar suspension solution assessed by hemocytometry under microscopy and the absorbance measured by light spectroscopy at a wavelength of 425 nm.

Fig. 4.

Pollen count per anther at different flowering stages and on stamens of different lengths among five oilseed rape cultivars. Data are presented as the means ± SEs. Identical lowercase letters indicate no significant difference (p>0.05); different lowercase letters indicate a significant difference (p<0.05).
Pollen count per anther at different flowering stages and on stamens of different lengths among five oilseed rape cultivars. Data are presented as the means ± SEs. Identical lowercase letters indicate no significant difference (p>0.05); different lowercase letters indicate a significant difference (p<0.05).

Number of pollen grains per flower in five oilseed rape cultivars

Cultivar Initial flowering stage Full bloom stage Final flowering stage
AYW 180524.23 ± 12083.10c 252715.64 ± 7947.12bc 136014.13 ± 5573.03c
HHY998 181954.12 ± 14039.44c 242706.37 ± 9721.72c 147306.64 ± 7737.18bc
ND21 276730.56 ± 12619.33a 270571.01 ± 8632.42b 215685.00 ± 9261.30a
DHY 217958.17 ± 13535.64b 314539.30 ± 9816.28a 168168.46 ± 8056.94b
HYXL 175464.60 ± 9152.36c 270131.04 ± 7004.21b 160029.05 ± 6928.05bc
P value 0.00 0.00 0.00
eISSN:
2299-4831
Język:
Angielski
Częstotliwość wydawania:
2 razy w roku
Dziedziny czasopisma:
Life Sciences, Zoology, other
Kanał RSS czasopisma