Absence of Nepotism in Waggle Communication of Honeybees (Apis mellifera )
, , , oraz | 24 paź 2020
O artykule
Article Category: Original Article
Data publikacji: 24 paź 2020
Zakres stron: 301 - 307
Otrzymano: 24 lut 2020
Przyjęty: 13 lip 2020
DOI: https://doi.org/10.2478/jas-2020-0021
Słowa kluczowe
© 2020 Zu Yun Zhang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Genotypes of paternity were in each colony
| Colony A | Colony B | ||||
|---|---|---|---|---|---|
| Subfamilies | Genotypes of paternity A014/A024/A113 | Random samples | Subfamilies | Genotypes of paternity A014/A024/A113 | Random samples |
| 1 | 225/120/215 | 18 | 1 | 215/120/215 | 6 |
| 2 | 225/120/235 | 12 | 2 | 215/120/210 | 4 |
| 3 | 225/120/225 | 9 | 3 | 235/120/215 | 8 |
| 4 | 215/120/235 | 8 | 4 | 220/120/215 | 7 |
| 5 | 215/120/215 | 6 | 5 | 225/120/215 | 5 |
| 6 | 215/120/225 | 2 | 6 | 215/120/235 | 5 |
| 7 | 245/120/215 | 2 | 7 | 235/110/225 | 5 |
| 8 | 235/120/225 | 2 | 8 | 235/120/235 | 3 |
| 9 | 225/110/215 | 1 | 9 | 245/120/215 | 0 |
| 10 | 235/120/235 | 2 | 10 | 235/120/210 | 1 |
| 11 | 245/110/225 | 0 | 11 | 220/120/235 | 3 |
| 12 | 245/120/235 | 2 | 12 | 220/120/210 | 1 |
| 13 | 235/110/235 | 0 | |||
| 14 | 235/110/215 | 0 | |||
Description of primer sequences and reaction condition used for microsatellites
| Locus | Sequence of primers | Allele size (bp) | Annealing temperature (°C) | No. cycles |
|---|---|---|---|---|
| A14 | F5′-GTGTCGCAATCGACGTAACC-3′ | 215/220/225/235245 | 58 | 30 |
| A24 | F5′-CACAAGTTCCAACAATGC-3′ | 110/120 | 55 | 30 |
| A113 | F5′-CTCGAATCGTGGCGTCC-3′ | 210/215/225/235 | 60 | 30 |
Distribution of super-sisters and half-sisters in colony A
| Dancer | Recruits | |||||
|---|---|---|---|---|---|---|
| No. | Subfamilies | Super-sister Observed | Half-sisters Observed | Super-sister Expected | Half-sisters Expected | P values |
| A1 | 5 | 1 | 11 | 1.1 | 10.9 | 1.000 |
| A2 | 7 | 0 | 11 | 0.3 | 10.7 | 1.000 |
| A3 | 7 | 1 | 8 | 0.3 | 8.7 | 1.000 |
| A4 | 6 | 0 | 8 | 0.2 | 7.8 | 1.000 |
| A5 | 3 | 3 | 4 | 1.0 | 6.0 | 0.559 |
| A6 | 1 | 1 | 6 | 2.0 | 5.0 | 1.000 |
| A7 | 2 | 2 | 4 | 1.1 | 4.9 | 1.000 |
| A8 | 1 | 3 | 6 | 2.5 | 6.5 | 1.000 |
| A9 | 6 | 5 | 2 | 0.2 | 6.8 | 0.021 |
| A10 | 10 | 1 | 6 | 0.2 | 6.8 | 1.000 |
| A11 | 3 | 1 | 10 | 1.6 | 9.4 | 1.000 |
| A12 | 6 | 1 | 12 | 0.4 | 12.6 | 1.000 |
| A13 | 5 | 3 | 7 | 0.9 | 9.1 | 0.582 |
Distribution of super-sisters and half-sisters in colony B
| Dancer | Recruits | |||||
|---|---|---|---|---|---|---|
| No. | Subfamilies | Super-sister Observed | Half-sisters Observed | Super-sister Expected | Half-sisters Expected | P values |
| B1 | 1 | 1 | 6 | 0.9 | 6.1 | 1.000 |
| B2 | 4 | 1 | 7 | 1.2 | 6.8 | 1.000 |
| B3 | 1 | 2 | 7 | 1.1 | 7.9 | 1.000 |
| B4 | 6 | 0 | 8 | 0.8 | 7.2 | 1.000 |
| B5 | 1 | 4 | 1 | 0.6 | 4.4 | 0.206 |
| B6 | 2 | 1 | 6 | 0.6 | 6.4 | 1.000 |
| B7 | 9 | 1 | 6 | 0 | 7.0 | 1.000 |
| B8 | 5 | 2 | 5 | 0.7 | 6.3 | 1.000 |
| B9 | 3 | 1 | 8 | 1.5 | 7.5 | 1.000 |
| B10 | 1 | 6 | 4 | 1.3 | 8.7 | 0.057 |
| B11 | 1 | 3 | 4 | 0.9 | 6.1 | 0.559 |
| B12 | 8 | 1 | 8 | 0.6 | 8.4 | 1.000 |