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In-silico analysis of mt-CO1 gene of Taenia hydatigena sheep isolates


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Fig. 1

The appearance of mt-CO1 (339 bp) haplotypes of sheep isolates of Cysticercus tenuicollis. Geographical distribution of the haplotypes is indicated by different colors. The size of the circles is related to the haplotype frequency. Number of mutations distinguishing the haplotypes is shown by hatch marks.
The appearance of mt-CO1 (339 bp) haplotypes of sheep isolates of Cysticercus tenuicollis. Geographical distribution of the haplotypes is indicated by different colors. The size of the circles is related to the haplotype frequency. Number of mutations distinguishing the haplotypes is shown by hatch marks.

Fig. 2

Phylogenetic tree view of sheep isolates using mt-CO1 gene (339 bp) sequences and reference sequences. MEGA X was used to construct a Maximum Likelihood tree based on the HKY+G model. The reliability of the tree was assessed by 1000 bootstrap replications.
Phylogenetic tree view of sheep isolates using mt-CO1 gene (339 bp) sequences and reference sequences. MEGA X was used to construct a Maximum Likelihood tree based on the HKY+G model. The reliability of the tree was assessed by 1000 bootstrap replications.

Grouping haplotypes of Cysticercus tenuicollis mt-CO1 sequences and accession numbers of isolates forming groups.

Haplotype name No of isolates Accession Numbers
Hap01 21 KM032303-Palestine, KM032302-Palestine, KM032300-Palestine, KM032299-Palestine, KM032298-Palestine, KM032289-Palestine, KM032288-Palestine, KM032287-Palestine, KM032286-Palestine, KM032285-Palestine, KM032284-Palestine, JQ710614-Iran, JQ710599- Iran, JQ710588-Iran, KT372530-Italy, KT372529-Italy, KT372522-Italy, MH113922-Iraq, MH113921-Iraq, MH113920-Iraq, MH113919-Iraq
Hap02 2 JQ710627-Iran, JQ710594-Iran
Hap03 1 JQ710626-Iran
Hap04 1 JQ710625-Iran
Hap05 1 JQ710624-Iran
Hap06 1 JQ710623-Iran
Hap07 1 JQ710622-Iran
Hap08 1 JQ710621-Iran
Hap09 1 JQ710620-Iran
Hap10 1 JQ710619-Iran
Hap11 1 JQ710618-Iran
Hap12 2 JQ710617-Iran, KM032294-Palestine
Hap13 1 JQ710616-Iran
Hap14 1 JQ710615-Iran
Hap15 1 JQ710613-Iran
Hap16 8 JQ710612-Iran, JQ710593-Iran, KT372528-Italy, KT372525-Italy, KT372524-Italy, KM032292-Palestine, KM032291-Palestine, DQ995656-India
Hap17 1 JQ710611-Iran
Hap18 1 JQ710610-Iran
Hap19 1 JQ710609-Iran
Hap20 1 JQ710608-Iran
Hap21 1 JQ710607-Iran
Hap22 1 JQ710606-Iran
Hap23 1 JQ710605-Iran
Hap24 2 JQ710604-Iran, KM032296-Palestine
Hap25 1 JQ710603-Iran
Hap26 1 JQ710602-Iran
Hap27 1 JQ710601-Iran
Hap28 2 JQ710600-Iran, JQ710591-Iran
Hap29 1 JQ710598-Iran
Hap30 1 JQ710597-Iran
Hap31 1 JQ710596-Iran
Hap32 1 JQ710595-Iran
Hap33 2 JQ710592-Iran, KT372520-Italy
Hap34 1 JQ710590-Iran
Hap35 3 JQ710589-Iran, KT372518-Italy, KM032301-Palestine
Hap36 1 KT372531-Italy
Hap37 1 KT372527-Italy
Hap38 1 KT372526-Italy
Hap39 1 KT372523-Italy
Hap40 1 KT372521-Italy
Hap41 1 KT372517-Italy
Hap42 1 KM032297-Palestine
Hap43 1 KM032295-Palestine
Hap44 1 KM032293-Palestine
Hap45 1 KM032290-Palestine
Hap46 1 EU544551-Finland
Hap47 2 NC012896-China, GQ228819-China

Access numbers of the mt-CO1 gene region of C. tenuicollis isolates used in the study.

Geographical region Number of isolates Accession Numbers Genbank Submit Date
China 2 NC012896 and GQ228819 2010 and 2016
Iran 40 JQ710588 - JQ710599 JQ710600 - JQ710627 2012
Palestine 20 KM032284 - KM032299 KM032300 - KM032303 2014
Italy (Sardinia) 14 KT372517 - KT372518 KT372520 - KT372531 2015
Finland 1 EU544551 2016
India 1 DQ995656 2016
Iraq 4 MH113919 - MH113922 2018

Total 82

Diversity and neutrality indices for Cysticercus tenuicollis isolates from Sardinia, Iran, Palestine, Iraq, Finland, India and China using nucleotide data of mt-CO1 gene (339 bp).

No of isolates (n) Polymorphic sites hn hd±SD πd ± SD Tajima’s D p value Fu’s Fs p value
Total (82) 44 47 0.926 ± 0.023 0.008 ± 0.0009 -2.2984 0.009* -60.528 0.000

Diversity and neutrality indices for Taenia hydatigena isolates from sheep originating from various geographical regions using nucleotide data of mt-CO1 (339 bp).

Geographical region (n) Polymorphic sites hn hd±SD nd ± SD Tajima’s D p value Fu’s Fs p value
Palestine (20) 10 9 0.705 ± 0.111 0.004 ± 0.0011 -1.610 0.093* -4.546 0.008
Iran (40) 36 35 0.992 ± 0.008 0.011 ± 0.001 -2.017 0.031* -44.411 0.000
Sardinia (14) 12 10 0.934 ± 0.051 0.007 ± 0.0013 -1.501 0.125* -5.775 0.003
eISSN:
1336-9083
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Zoology, Ecology, other, Medicine, Clinical Medicine, Microbiology, Virology and Infection Epidemiology