In this paper, we study a class of univalent functions f as defined by making use of the generalized Ruscheweyh derivatives involving a general fractional derivative operator, satisfying
{\mathop{\rm Re}\nolimits} \left\{{{{z\left({{\bf{J}}_1^{\lambda,\mu}f\left(z \right)} \right)'} \over {\left({1 - \gamma} \right){\bf{J}}_1^{\lambda,\mu}f\left(z \right) + \gamma {z^2}\left({{\bf{J}}_1^{\lambda,\mu}f\left(z \right)} \right)''}}} \right\} > \beta.
A necessary and sufficient condition for a function to be in the class A_\gamma ^{\lambda,\mu,\nu}\left({n,\beta} \right) is obtained. Also, our paper includes linear combination, integral operators and we introduce the subclass A_{\gamma,{c_m}}^{\lambda,\mu,\nu}\left({1,\beta} \right) consisting of functions with negative and fixed finitely many coefficients. We study some interesting properties of A_{\gamma,{c_m}}^{\lambda,\mu,\nu}\left({1,\beta} \right).