<abstract xmlns="http://www.w3.org/1999/xhtml">
Norway spruce is expected to suffer from drought stress and other manifestations of climate change. This study relies on a manipulative experiment with drought-stressed and well-watered (control) seedlings, comprising five provenances of Norway spruce distributed along a steep elevational transect from 550 to 1,280 m a.s.l. within the natural range. Seedlings were subjected to measurement of physiological traits (content of phytohormones and monoterpenes, slow and fast chlorophyll <italic>a</italic> fluorescence kinetics, gas exchange, hyperspectral indices), and genotyping at 8 nuclear microsatellite loci. Comparison of the coefficient of differentiation at neutral loci (<italic>FST</italic>) vs. differentiation at phenotypic traits (<italic>PST</italic>) was used to identify traits underlying divergent selection. In total, 18 traits exhibited a significant <italic>PST – FST</italic> difference. However, the consistency in differentiation patterns between drought-stressed and control plants was limited, only three traits exhibited signals of selection under both treatments. This outcome indicates that the identified differentiation patterns can only be interpreted in the context of environmental setup of the experiment, and highlights the importance of common gardens in adaptation research, as they allow both elimination of environment-induced phenotypic variation and studying genotype-by-environment interaction in physiological responses to environmental stresses.
</abstract>

Norway spruce is expected to suffer from drought stress and other manifestations of climate change. This study relies on a manipulative experiment with drought-stressed and well-watered (control) seedlings, comprising five provenances of Norway spruce distributed along a steep elevational transect from 550 to 1,280 m a.s.l. within the natural range. Seedlings were subjected to measurement of physiological traits (content of phytohormones and monoterpenes, slow and fast chlorophyll a fluorescence kinetics, gas exchange, hyperspectral indices), and genotyping at 8 nuclear microsatellite loci. Comparison of the coefficient of differentiation at neutral loci (FST) vs. differentiation at phenotypic traits (PST) was used to identify traits underlying divergent selection. In total, 18 traits exhibited a significant PST – FST difference. However, the consistency in differentiation patterns between drought-stressed and control plants was limited, only three traits exhibited signals of selection under both treatments. This outcome indicates that the identified differentiation patterns can only be interpreted in the context of environmental setup of the experiment, and highlights the importance of common gardens in adaptation research, as they allow both elimination of environment-induced phenotypic variation and studying genotype-by-environment interaction in physiological responses to environmental stresses.

Inconsistent phenotypic differentiation at physiological traits in Norway spruce (<italic>Picea abies </italic>Karst.) provenances under contrasting water regimes

Central European Forestry Journal

This work is licensed under the Creative Commons Attribution 4.0 International License.

Inconsistent phenotypic differentiation at physiological traits in Norway spruce (Karst.) provenances under contrasting water regimes

Norway spruce is expected to suffer from drought stress and other manifestations of climate change. This study relies on a manipulative experiment with...

Inconsistent phenotypic differentiation at physiological traits in Norway spruce (Picea abies Karst.) provenances under contrasting water regimes

Data publikacji: 19 paź 2023

Zakres stron: 214 - 223

DOI: https://doi.org/10.2478/forj-2023-0010

Słowa kluczowe
adaptation, phenotypic differentiation, drought stress, plasticity, divergent selection

© 2023 Dušan Gömöry et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Inconsistent phenotypic differentiation at physiological traits in Norway spruce (Picea abies Karst.) provenances under contrasting water regimes

Data publikacji: 19 paź 2023

Zakres stron: 214 - 223

DOI: https://doi.org/10.2478/forj-2023-0010

Słowa kluczoweadaptation, phenotypic differentiation, drought stress, plasticity, divergent selection

© 2023 Dušan Gömöry et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Słowa kluczowe
adaptation, phenotypic differentiation, drought stress, plasticity, divergent selection