Sample code | Primer | The strain with the highest compatibility | Compatibility (%) | NCBI access number |
---|---|---|---|---|
16–31 | 63F | 100 | NR_117624.1 | |
1387R | Strain ATCC 17909 | 99 | ||
16–35 | 63F | 100 | NR_041028.1 | |
1387R | Strain DSM 5687 | 100 |
Volume (μl) | Final concentration | |
---|---|---|
GoTaq Green Master mix 2x | 25 | 1× |
Upstream primer 63F | 5 | 1 μM |
Downstream primer 1387R | 5 | 1 μM |
Genomic DNA | 5 | < 250 ng |
Nuclease-free water | 10 | – |
Total | 50 |
Antibiotic class | Antibiotic | AJ | MR |
---|---|---|---|
β-lactams | Penicillin | R | S |
Amoxicillin | R | S | |
Ampicillin+sulbactam | R | S | |
Ampicillin | R | S | |
Piperacillin | S | S | |
Tikarcillin | S | S | |
Cefuroxime | R | S | |
Tetracyclines | Doksycycline | S | S |
Tetracycline | S | S | |
Fluoroquinolones | Ciprofloxacin | S | S |
Levofloxacin | S | S | |
Norfloxacin | S | S | |
Ofloxacin | S | S | |
Aminoglycosides | Amikacina | S | S |
Gentamicin | S | S | |
Netilmicin | S | S | |
Tobramycin | S | S | |
Macrolides, Lincosamides, Streptogramins | Azithromycin | R | S |
Clarithromycin | R | S | |
Erythromycin | R | S | |
Clindamycin | R | R | |
Others | Chloramphenicol | R | S |
Nitrofurantoin | R | R | |
Trimethoprim | R | R | |
Co-trimoxazole | S | R |