University of Lille, Inserm, University Hospital Center (CHU) Lille, U1192-Proteomics Inflammatory Response Mass Spectrometry (PRISM), Lille, FranceInstitut Universitaire de
France, Ministry of Higher Education, Research and Innovation, 1 rue DescartesParis, France
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Wang Y, Jia J, Wang F, Fang Y, Yang Y, et al. (2024) Pre-metastatic niche: formation, characteristics and therapeutic implication. Signal Transduct Target Ther 9: 236.Search in Google Scholar
Jin Y, Xing J, Xu K, Liu D, Zhuo Y (2022) Exosomes in the tumor microenvironment: Promoting cancer progression. Front Immunol 13: 1025218.Search in Google Scholar
Hanahan D, Coussens LM (2012) Accessories to the Crime: Functions of Cells Recruited to the Tumor Micro-environment. Cancer Cell 21: 309–322.Search in Google Scholar
Kalluri R, LeBleu VS (2020) The biology, function, and biomedical applications of exosomes. Science (80- ) 367: eaau6977.Search in Google Scholar
Mashouri L, Yousefi H, Aref AR, Ahadi AM, Molaei F, et al. (2019) Exosomes: Composition, biogenesis, and mechanisms in cancer metastasis and drug resistance. Mol Cancer 18: 1–14.Search in Google Scholar
Liu Y, Cao X (2016) Characteristics and Significance of the Pre-metastatic Niche. Cancer Cell 30: 668–681.Search in Google Scholar
Han QF, Li WJ, Hu KS, Gao J, Zhai WL, et al. (2022) Exo-some biogenesis: machinery, regulation, and therapeutic implications in cancer. Mol Cancer 21: 1–26.Search in Google Scholar
Juan T, Fürthauer M (2018) Biogenesis and function of ESCRT-dependent extracellular vesicles. Semin Cell Dev Biol 74: 66–77.Search in Google Scholar
Lee YJ, Shin KJ, Chae YC (2024) Regulation of cargo selection in exosome biogenesis and its biomedical applications in cancer. Exp Mol Med 56: 877–889.Search in Google Scholar
Jin H, Tang Y, Yang L, Peng X, Li B, et al. (2021) Rab GTPases: Central Coordinators of Membrane Trafficking in Cancer. Front Cell Dev Biol 9: 648384.Search in Google Scholar
Larios J, Mercier V, Roux A, Gruenberg J (2020) ALIXAnd ESCRT-III-dependent sorting of tetraspanins to exosomes. J Cell Biol 219: e201904113.Search in Google Scholar
Bishop N, Horman A, Woodman P (2002) Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates. J Cell Biol 157: 91–101.Search in Google Scholar
Baietti MF, Zhang Z, Mortier E, Melchior A, Degeest G, et al. (2012) Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nat Cell Biol 14: 677–685.Search in Google Scholar
Lee KM, Seo EC, Lee JH, Kim HJ, Hwangbo C (2023) The Multifunctional Protein Syntenin-1: Regulator of Exosome Biogenesis, Cellular Function, and Tumor Progression. Int J Mol Sci 24: 9418.Search in Google Scholar
Hwangbo C, Park J, Lee JH (2011) mda-9/syntenin protein positively regulates the activation of Akt protein by facilitating integrin-linked kinase adaptor function during adhesion to type I collagen. J Biol Chem 286: 33601–33612.Search in Google Scholar
Zou W, Lai M, Zhang Y, Zheng L, Xing Z, et al. (2019) Exosome Release Is Regulated by mTORC1. Adv Sci 6: 1801313.Search in Google Scholar
Yu M, Zhang J, Fu J, Li S, Cui X (2025) Guizhi Fuling decoction protects against bone destruction via suppressing exosomal ERK1 in multiple myeloma. Phytomedicine 140: 156627.Search in Google Scholar
Wang L, Yan Z, Xia Y (2023) Silencing RAB27a inhibits proliferation, invasion and adhesion of triple-negative breast cancer cells. Nan Fang Yi Ke Da Xue Xue Bao / J South Med Univ 43: 560–567.Search in Google Scholar
Park JI, Song KH, Kang SM, Lee J, Cho SJ, et al. (2022) BHMPS Inhibits Breast Cancer Migration and Invasion by Disrupting Rab27a⁺Mediated EGFR and Fibronectin Secretion. Cancers (Basel) 14: 373.Search in Google Scholar
Peng Y, Zhao M, Hu Y, Guo H, Zhang Y, et al. (2022) Blockade of exosome generation by GW4869 inhibits the education of M2 macrophages in prostate cancer. BMC Immunol 23: 1–15.Search in Google Scholar
Cheng Q, Li X, Wang Y, Dong M, Zhan FH, et al. (2018) The ceramide pathway is involved in the survival, apoptosis and exosome functions of human multiple myeloma cells in vitro. Acta Pharmacol Sin 39: 561–568.Search in Google Scholar
Ghaemmaghami AB, Mahjoubin-Tehran M, Movahed-pour A, Morshedi K, Sheida A, et al. (2020) Role of exosomes in malignant glioma: MicroRNAs and proteins in pathogenesis and diagnosis. Cell Commun Signal 18: 120.Search in Google Scholar
Schuurmans C, Balakrishnan A, Roy S, Fleming T, Leong HS (2020) The emerging role of extracellular vesicles in the glioma microenvironment: Biogenesis and clinical relevance. Cancers (Basel) 12: 1–25.Search in Google Scholar
Das A, Saha P, Kalele K, Sonar S (2024) Clinical signature of exosomal tetraspanin proteins in cancer. Clin Transl Discov 4: e341.Search in Google Scholar
Jankovičová J, Sečová P, Michalková K, Antalíková J (2020) Tetraspanins, more than markers of extracellular vesicles in reproduction. Int J Mol Sci 21: 1–30.Search in Google Scholar
Hurwitz SN, Olcese JM, Meckes DG (2019) Extraction of Extracellular Vesicles from Whole Tissue. J Vis Exp 2019.Search in Google Scholar
Mazurov D, Barbashova L, Filatov A (2013) Tetraspanin protein CD9 interacts with metalloprotease CD10 and enhances its release via exosomes. FEBS J 280: 1200–1213.Search in Google Scholar
Saito RF, Machado CML, Lomba ALO, Otake AH, Rangel MC (2024) Heat Shock Proteins Mediate Intercellular Communications within the Tumor Microenvironment through Extracellular Vesicles. Appl Biosci 3: 45–58.Search in Google Scholar
Gastpar R, Gehrmann M, Bausero MA, Asea A, Gross C, et al. (2005) Heat shock protein 70 surface-positive tumor exosomes stimulate migratory and cytolytic activity of natural killer cells. Cancer Res 65: 5238–5247.Search in Google Scholar
Wang F, Bashiri Dezfouli A, Multhoff G (2024) The immunomodulatory effects of cannabidiol on Hsp70-activated NK cells and tumor target cells. Mol Immunol 174: 1–10.Search in Google Scholar
Krysko D V., Garg AD, Kaczmarek A, Krysko O, Agostinis P, et al. (2012) Immunogenic cell death and DAMPs in cancer therapy. Nat Rev Cancer 12: 860–875.Search in Google Scholar
Zhou J, Wang G, Chen Y, Wang H, Hua Y, et al. (2019) Immunogenic cell death in cancer therapy: Present and emerging inducers. J Cell Mol Med 23: 4854–4865.Search in Google Scholar
Vasaturo A, Di Blasio S, Peeters DGA, de Koning CCH, de Vries JM, et al. (2013) Clinical implications of co-inhibitory molecule expression in the tumor microenvironment for DC vaccination: A game of stop and go. Front Immunol 4.Search in Google Scholar
Menger L, Vacchelli E, Adjemian S, Martins I, Ma Y, et al. (2012) Cardiac glycosides exert anticancer effects by inducing immunogenic cell death. Sci Transl Med 4.Search in Google Scholar
Arya SB, Chen S, Jordan-Javed F, Parent CA (2022) Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation. Nat Cell Biol 24: 1019–1028.Search in Google Scholar
Abdullah M, Nakamura T, Ferdous T, Gao Y, Chen Y, et al. (2021) Cholesterol Regulates Exosome Release in Cultured Astrocytes. Front Immunol 12: 722581.Search in Google Scholar
Zhuo Y, Luo Z, Zhu Z, Wang J, Li X, et al. (2024) Direct cytosolic delivery of siRNA via cell membrane fusion using cholesterol-enriched exosomes. Nat Nanotechnol 19: 1858–1868.Search in Google Scholar
Sharma R, Huang X, Brekken RA, Schroit AJ (2017) Detection of phosphatidylserine-positive exosomes for the diagnosis of early-stage malignancies. Br J Cancer 117: 545–552.Search in Google Scholar
Birge RB, Boeltz S, Kumar S, Carlson J, Wanderley J, et al. (2016) Phosphatidylserine is a global immunosuppressive signal in efferocytosis, infectious disease, and cancer. Cell Death Differ 23: 962–978.Search in Google Scholar
Bhatta M, Shenoy GN, Loyall JL, Gray BD, Bapardekar M, et al. (2021) Novel phosphatidylserine-binding molecule enhances antitumor T-cell responses by targeting immunosuppressive exosomes in human tumor microenvironments. J Immunother Cancer 9: 3148.Search in Google Scholar
Tan S, Xia L, Yi P, Han Y, Tang L, et al. (2020) Exosomal miRNAs in tumor microenvironment. J Exp Clin Cancer Res 39: 1–15.Search in Google Scholar
Kenanoglu S, Akalin H, Aslan D, Inanc M, Ozturk F, et al. (2024) Insights into multidrug resistance mechanisms: Exploring distinct miRNAs as prospective therapeutic agents in triple negative breast cancer. Gene Reports 37.Search in Google Scholar
Zhang Z, Xing T, Chen Y, Xiao J (2018) Exosome-mediated miR-200b promotes colorectal cancer proliferation upon TGF-β1 exposure. Biomed Pharmacother 106: 1135–1143.Search in Google Scholar
Zhang P, Zhou H, Lu K, Lu Y, Wang Y, et al. (2018) Exo-some-mediated delivery of MALATI induces cell proliferation in breast cancer. Onco Targets Ther 11: 291–299.Search in Google Scholar
Santos JC, Ribeiro ML, Sarian LO, Ortega MM, Derchain SF (2016) Exosomes-mediate microRNAs transfer in breast cancer chemoresistance regulation. Am J Cancer Res 6: 2129–2139.Search in Google Scholar
Zhang HG, Grizzle WE (2011) Exosomes and cancer: A newly described pathway of immune suppression. Clin Cancer Res 17: 959–964.Search in Google Scholar
Jung KO, Youn H, Lee CH, Kang KW, Chung JK (2017) Visualization of exosome-mediated miR-210 transfer from hypoxic tumor cells. Oncotarget 8: 9899–9910.Search in Google Scholar
Tsuda M, Fukuda A, Roy N, Hiramatsu Y, Leonhardt L, et al. (2018) The BRG1/SOX9 axis is critical for acinar cell-derived pancreatic tumorigenesis. J Clin Invest 128: 3475–3489.Search in Google Scholar
Zhao C, Li X, Han B, Qu L, Liu C, et al. (2018) Gga-miR-130b-3p inhibits MSB1 cell proliferation, migration, invasion, and its downregulation in MD tumor is attributed to hypermethylation. Oncotarget 9.Search in Google Scholar
Yan YF, Gong FM, Wang BS, Zheng W (2017) MiR-425-5p promotes tumor progression via modulation of CYLD in gastric cancer. Eur Rev Med Pharmacol Sci 21.Search in Google Scholar
Quan J, Li Y, Pan X, Lai Y, He T, et al. (2018) Oncogenic mir-425-5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma. Oncol Lett 16.Search in Google Scholar
Xu WX, Wang DD, Zhao ZQ, Zhang H Da, Yang SJ, et al. (2022) Exosomal microRNAs shuttling between tumor cells and macrophages: cellular interactions and novel therapeutic strategies. Cancer Cell Int 22: 1–9.Search in Google Scholar
Cantile M, Di Bonito M, Cerrone M, Collina F, De Lauren-tiis M, et al. (2020) Long non-coding rna hotair in breast cancer therapy. Cancers (Basel) 12.Search in Google Scholar
Wang S, Chen W, Yu H, Song Z, Li Q, et al. (2020) lncRNA ROR Promotes Gastric Cancer Drug Resistance. Cancer Control 27.Search in Google Scholar
Meng X, Yang D, Zhang B, Zhao Y, Zheng Z, et al. (2023) Regulatory mechanisms and clinical applications of tumor-driven exosomal circrnas in cancers. Int J Med Sci 20: 818–835.Search in Google Scholar
Lu MM, Yang Y (2024) Exosomal PD-L1 in cancer and other fields: recent advances and perspectives. Front Immunol 15: 1395332.Search in Google Scholar
Chen G, Huang AC, Zhang W, Zhang G, Wu M, et al. (2018) Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response. Nature 560: 382–386.Search in Google Scholar
Yu ZL, Liu JY, Chen G (2022) Small extracellular vesicle PD-L1 in cancer: the knowns and unknowns. npj Precis Oncol 6: 1–15.Search in Google Scholar
Tavakkoli S, Sotoodehnejadnematalahi F, Fathollahi A, Bandehpour M, Hoseini MHM, et al. (2020) EL4-derived Exosomes Carry Functional TNF-related Apoptosisinducing Ligand that are Able to Induce Apoptosis and Necrosis in the Target Cells. Int J Mol Cell Med 9: 207–214.Search in Google Scholar
Meng Y, Graves L, Do TV, So J, Fishman DA (2004) Up-regulation of FasL by LPA on ovarian cancer cell surface leads to apoptosis of activated lymphocytes. Gynecol Oncol 95: 488–495.Search in Google Scholar
Stenqvist A-C, Nagaeva O, Baranov V, Mincheva-Nilsson L (2013) Exosomes Secreted by Human Placenta Carry Functional Fas Ligand and TRAIL Molecules and Convey Apoptosis in Activated Immune Cells, Suggesting Exo-some-Mediated Immune Privilege of the Fetus. J Immunol 191: 5515–5523.Search in Google Scholar
Otmani K, Rouas R, Lagneaux L, Krayem M, Duvillier H, et al. (2023) Acute myeloid leukemia-derived exosomes deliver miR-24-3p to hinder the T-cell immune response through DENN/MADD targeting in the NF-κB signaling pathways. Cell Commun Signal 21: 253.Search in Google Scholar
Ye B, Duan Y, Zhou M, Wang Y, Lai Q, et al. (2023) Hypoxic tumor-derived exosomal miR-21 induces cancer-associated fibroblast activation to promote head and neck squamous cell carcinoma metastasis. Cell Signal 108.Search in Google Scholar
Mizuhara K, Shimura Y, Tsukamoto T, Kanai A, Kuwahara-Ota S, et al. (2023) Tumour-derived exosomes promote the induction of monocytic myeloid-derived suppressor cells from peripheral blood mononuclear cells by delivering miR-106a-5p and miR-146a-5p in multiple myeloma. Br J Haematol 203: 426–438.Search in Google Scholar
Ding G, Zhou L, Qian Y, Fu M, Chen J, et al. (2015) Pancreatic cancer-derived exosomes transfer miRNAs to dendritic cells and inhibit RFXAP expression via miR-212-3p. Oncotarget 6: 29877–29888.Search in Google Scholar
Fabbri M, Paone A, Calore F, Galli R, Gaudio E, et al. (2012) MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response. Proc Natl Acad Sci U S A 109: E2110–E2116.Search in Google Scholar
Wang S, Ma F, Feng Y, Liu T, He S (2020) Role of exosomal miR–21 in the tumor microenvironment and osteosarcoma tumorigenesis and progression (Review). Int J Oncol 56: 1055–1063.Search in Google Scholar
Yan Z, Wen J-X, Cao X-S, Zhao W, Han Y-L, et al. (2022) Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization. Ann Transl Med 10: 1307–1307.Search in Google Scholar
Clayton A, Mitchell JP, Court J, Linnane S, Mason MD, et al. (2008) Human Tumor-Derived Exosomes Down-Modulate NKG2D Expression. J Immunol 180: 7249–7258.Search in Google Scholar
Hong CS, Sharma P, Yerneni SS, Simms P, Jackson EK, et al. (2017) Circulating exosomes carrying an immunosuppressive cargo interfere with cellular immunotherapy in acute myeloid leukemia. Sci Rep 7.Search in Google Scholar
Zhao J, Schlößer HA, Wang Z, Qin J, Li J, et al. (2019) Tumor-derived extracellular vesicles inhibit natural killer cell function in pancreatic cancer. Cancers (Basel) 11.Search in Google Scholar
Moloudizargari M, Redegeld F, Asghari MH, Mosaffa N, Mortaz E (2020) Long-chain polyunsaturated omega-3 fatty acids reduce multiple myeloma exosome-mediated suppression of NK cell cytotoxicity. DARU, J Pharm Sci 28: 647–659.Search in Google Scholar
Shoae-Hassani A, Hamidieh AA, Behfar M, Mohseni R, Mortazavi-Tabatabaei SA, et al. (2017) NK Cell-derived Exosomes from NK Cells Previously Exposed to Neuroblastoma Cells Augment the Antitumor Activity of Cytokine-Activated NK Cells. J Immunother 40: 265–276.Search in Google Scholar
Siemaszko J, Marzec⁺przyszlak A, Bogunia⁺kubik K (2021) Nkg2d natural killer cell receptor—a short description and potential clinical applications. Cells 10: 1420.Search in Google Scholar
Kaifu T, Escalière B, Gastinel LN, Vivier E, Baratin M (2011) B7-H6/NKp30 interaction: A mechanism of alerting NK cells against tumors. Cell Mol Life Sci 68: 3531–3539.Search in Google Scholar
Regis S, Dondero A, Caliendo F, Bottino C, Castriconi R (2020) NK Cell Function Regulation by TGF-β-Induced Epigenetic Mechanisms. Front Immunol 11: 497915.Search in Google Scholar
Mamessier E, Sylvain A, Thibult ML, Houvenaeghel G, Jacquemier J, et al. (2011) Human breast cancer cells enhance self tolerance by promoting evasion from NK cell antitumor immunity. J Clin Invest 121: 3609–3622.Search in Google Scholar
Hosseini R, Asef-Kabiri L, Yousefi H, Sarvnaz H, Salehi M, et al. (2021) The roles of tumor-derived exosomes in altered differentiation, maturation and function of dendritic cells. Mol Cancer 20: 83.Search in Google Scholar
Ding G, Zhou L, Qian Y, Fu M, Chen J, et al. (2015) Pancreatic cancer-derived exosomes transfer miRNAs to dendritic cells and inhibit RFXAP expression via miR-212-3p. Oncotarget 6: 29877–29888.Search in Google Scholar
Li H, Chen X, Zheng S, Han B, Zhang X, et al. (2024) The expansion of MDSCs induced by exosomal PD-L1 promotes the progression of gastric cancer. J Transl Med 22: 1–15.Search in Google Scholar
Irep N, Inci K, Tokgun PE, Tokgun O (2024) Exosome inhibition improves response to first-line therapy in small cell lung cancer. J Cell Mol Med 28: e18138.Search in Google Scholar
Sonar S, Das A, Kalele K, Subramaniyan V (2025) Exo-some-based cancer vaccine: a cell-free approach. Mol Biol Rep 52.Search in Google Scholar
Gao J, Zhang X, Jiang L, Li Y, Zheng Q (2022) Tumor endothelial cell-derived extracellular vesicles contribute to tumor microenvironment remodeling. Cell Commun Signal 20: 97.Search in Google Scholar
Treps L, Perret R, Edmond S, Ricard D, Gavard J (2017) Glioblastoma stem-like cells secrete the pro-angiogenic VEGF-A factor in extracellular vesicles. J Extracell Vesicles 6.Search in Google Scholar
Zhou W, Fong MY, Min Y, Somlo G, Liu L, et al. (2014) Cancer-Secreted miR-105 destroys vascular endothelial barriers to promote metastasis. Cancer Cell 25: 501–515.Search in Google Scholar
Huang XY, Huang ZL, Huang J, Xu B, Huang XY, et al. (2020) Exosomal circRNA-100338 promotes hepatocellular carcinoma metastasis via enhancing invasiveness and angiogenesis. J Exp Clin Cancer Res 39.Search in Google Scholar
Hood JL, San Roman S, Wickline SA (2011) Exosomes released by melanoma cells prepare sentinel lymph nodes for tumor metastasis. Cancer Res 71: 3792–3801.Search in Google Scholar
Gintoni I, Vassiliou S, Chrousos GP, Yapijakis C (2023) Review of Disease-Specific microRNAs by Strategically Bridging Genetics and Epigenetics in Oral Squamous Cell Carcinoma. Genes (Basel) 14: 1578.Search in Google Scholar
Wang W, Kong P, Feng K, Liu C, Gong X, et al. (2023) Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling. Cancer Sci 114: 4252–4269.Search in Google Scholar
Zeng Z, Li Y, Pan Y, Lan X, Song F, et al. (2018) Cancer-derived exosomal miR-25-3p promotes pre-metastatic niche formation by inducing vascular permeability and angio-genesis. Nat Commun 9: 1–14.Search in Google Scholar
Duan SL, Fu WJ, Jiang YK, Peng LS, Ousmane D, et al. (2023) Emerging role of exosome-derived non-coding RNAs in tumor-associated angiogenesis of tumor micro-environment. Front Mol Biosci 10: 1220193.Search in Google Scholar
Wang D, Li H, Zeng T, Chen Q, Huang W, et al. (2024) Exosome-transmitted ANGPTL1 suppresses angiogenesis in glioblastoma by inhibiting the VEGFA/VEGFR2/Akt/eNOS pathway. J Neuroimmunol 387.Search in Google Scholar
Nedaeinia R, Najafgholian S, Salehi R, Goli M, Ranjbar M, et al. (2024) The role of cancer-associated fibroblasts and exosomal miRNAs-mediated intercellular communication in the tumor microenvironment and the biology of carcinogenesis: a systematic review. Cell Death Discov 10: 1–20.Search in Google Scholar
Peng Z, Tong Z, Ren Z, Ye M, Hu K (2023) Cancer-associated fibroblasts and its derived exosomes: a new perspective for reshaping the tumor microenvironment. Mol Med 29: 66.Search in Google Scholar
Baroni S, Romero-Cordoba S, Plantamura I, Dugo M, D’Ippolito E, et al. (2016) Exosome-mediated delivery of miR-9 induces cancer-Associated fibroblast-like properties in human breast fibroblasts. Cell Death Dis 7: e2312–e2312.Search in Google Scholar
Wang J, Guan X, Zhang Y, Ge S, Zhang L, et al. (2018) Exosomal miR-27a derived from gastric cancer cells regulates the transformation of fibroblasts into cancer-associated fibroblasts. Cell Physiol Biochem 49: 869–883.Search in Google Scholar
De Lellis L, Florio R, Di Bella MC, Brocco D, Guidotti F, et al. (2021) Exosomes as pleiotropic players in pancreatic cancer. Biomedicines 9: 1–26.Search in Google Scholar
Pang W, Su J, Wang Y, Feng H, Dai X, et al. (2015) Pancreatic cancer-secreted miR-155 implicates in the conversion from normal fibroblasts to cancer-associated fibroblasts. Cancer Sci 106: 1362–1369.Search in Google Scholar
Fang X, Lan H, Jin K, Qian J (2023) Pancreatic cancer and exosomes: role in progression, diagnosis, monitoring, and treatment. Front Oncol 13: 1149551.Search in Google Scholar
Fang Z, Xu J, Zhang B, Wang W, Liu J, et al. (2020) The promising role of noncoding RNAs in cancer-associated fibroblasts: an overview of current status and future perspectives. J Hematol Oncol 13: 154.Search in Google Scholar
Nedaeinia R, Najafgholian S, Salehi R, Goli M, Ranjbar M, et al. (2024) The role of cancer-associated fibroblasts and exosomal miRNAs-mediated intercellular communication in the tumor microenvironment and the biology of carcinogenesis: a systematic review. Cell Death Discov 10: 380.Search in Google Scholar
Webber J, Steadman R, Mason MD, Tabi Z, Clayton A (2010) Cancer exosomes trigger fibroblast to myofibro-blast differentiation. Cancer Res 70: 9621–9630.Search in Google Scholar
Perkins RS, Singh R, Abell AN, Krum SA, Miranda-Carboni GA (2023) The role of WNT10B in physiology and disease: A 10-year update. Front Cell Dev Biol 11: 1120365.Search in Google Scholar
Qin X, Guo H, Wang X, Zhu X, Yan M, et al. (2019) Exosomal miR-196a derived from cancer-associated fibro-blasts confers cisplatin resistance in head and neck cancer through targeting CDKN1B and ING5. Genome Biol 20.Search in Google Scholar
Wu Y, Xiao Y, Ding Y, Ran R, Wei K, et al. (2024) Colorectal cancer cell-derived exosomal miRNA-372-5p induces immune escape from colorectal cancer via PTEN/AKT/NF-κB/PD-L1 pathway. Int Immunopharmacol 143.Search in Google Scholar
Zhou C, Wei W, Ma J, Yang Y, Liang L, et al. (2021) Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels. Mol Ther 29: 1512–1528.Search in Google Scholar
Vignard V, Labbe M, Marec N, Andre-Gregoire G, Jouand N, et al. (2020) MicroRNAs in tumor exosomes drive immune escape in melanoma. Cancer Immunol Res 8: 255–267.Search in Google Scholar
Wu Y, Yi M, Niu M, Mei Q, Wu K (2022) Myeloid-derived suppressor cells: an emerging target for anticancer immunotherapy. Mol Cancer 21: 1–19.Search in Google Scholar
Liang L, Xu X, Li J, Yang C (2022) Interaction Between microRNAs and Myeloid-Derived Suppressor Cells in Tumor Microenvironment. Front Immunol 13: 883683.Search in Google Scholar
Ren WH, Zhang XR, Li WB, Feng Q, Feng HJ, et al. (2019) Exosomal miRNA-107 induces myeloid-derived suppressor cell expansion in gastric cancer. Cancer Manag Res 11: 4023–4040.Search in Google Scholar
Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, et al. (2018) Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246. Nat Commun 9: 1–15.Search in Google Scholar
Zeng Y, Fu BM (2024) Angiogenesis and Microvascular Permeability. Cold Spring Harb Perspect Med 15: a041163.Search in Google Scholar
Sun J, Luo J, Liu J, Wu H, Li Y, et al. (2025) Cancer-secreted exosomal miR-1825 induces angiogenesis to promote colorectal cancer metastasis. Cancer Cell Int 25: 1–14.Search in Google Scholar
Chen Y, Zhou Y, Chen J, Yang J, Yuan Y, et al. (2024) Exosomal lncRNA SNHG12 promotes angiogenesis and breast cancer progression. Breast Cancer 31: 607–620.Search in Google Scholar
Zhou T, Lu P (2024) Exosomal microRNA-21-5p from gastric cancer cells promotes angiogenesis by targeting LEMD3 in human endothelial cells. Oncologie 26: 983–992.Search in Google Scholar
Xie M, Yu T, Jing X, Ma L, Fan Y, et al. (2020) Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation. Mol Cancer 19: 1–22.Search in Google Scholar
Lang HL, Hu GW, Zhang B, Kuang W, Chen Y, et al. (2017) Glioma cells enhance angiogenesis and inhibit endothelial cell apoptosis through the release of exosomes that contain long non-coding RNA CCAT2. Oncol Rep 38: 785–798.Search in Google Scholar
Chen JJ, Zhou SH (2011) Mesenchymal stem cells overex-pressing MiR-126 enhance ischemic angiogenesis via the AKT/ERK-related pathway. Cardiol J 18: 675–681.Search in Google Scholar
Bhattacharya B, Nag S, Mukherjee S, Kulkarni M, Chan-dane P, et al. (2024) Role of Exosomes in Epithelial-Mesenchymal Transition. ACS Appl Bio Mater 7: 44–58.Search in Google Scholar
Moshiri F, Salvi A, Gramantieri L, Sangiovanni A, Guerriero P, et al. (2018) Circulating miR-106b-3p, miR-101-3p and miR-1246 as diagnostic biomarkers of hepatocellular carcinoma. Oncotarget 9: 15350–15364.Search in Google Scholar
Guo X, Zhang Y, Liu L, Yang W, Zhang Q (2020) Hnf1a-as1 regulates cell migration, invasion and glycolysis via modulating mir-124/myo6 in colorectal cancer cells. Onco Targets Ther 13: 1507–1518.Search in Google Scholar
Zhan Y, Li Y, Guan B, Wang Z, Peng D, et al. (2017) Long non-coding RNA HNF1A-AS1 promotes proliferation and suppresses apoptosis of bladder cancer cells through up-regulating Bcl-2. Oncotarget 8: 76656–76665.Search in Google Scholar
Georgantzoglou N, Pergaris A, Masaoutis C, Theocharis S (2021) Extracellular vesicles as biomarkers carriers in bladder cancer: Diagnosis, surveillance, and treatment. Int J Mol Sci 22: 1–15.Search in Google Scholar
Liu Y, Zhao F, Tan F, Tang L, Du Z, et al. (2022) HNF1A-AS1: A Tumor-associated Long Non-coding RNA. Curr Pharm Des 28: 1720–1729.Search in Google Scholar
Li Z, Chen Z, Li S, Qian X, Zhang L, et al. (2023) Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA. Cell Death Discov 9: 1–14.Search in Google Scholar
Wang K, Chen Z, Qiao X, Zheng J (2023) Hsa_circ_0084003 modulates glycolysis and epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma through targeting hsa-miR-143-3p/DNMT3A axis. Toxicol Res (Camb) 12: 457–467.Search in Google Scholar
Yoshimura A, Sawada K, Nakamura K, Kinose Y, Nakatsuka E, et al. (2018) Exosomal miR-99a-5p is elevated in sera of ovarian cancer patients and promotes cancer cell invasion by increasing fibronectin and vitronectin expression in neighboring peritoneal mesothelial cells. BMC Cancer 18: 1–13.Search in Google Scholar
Qu K, Lin T, Pang Q, Liu T, Wang Z, et al. (2016) Extracellular miRNA-21 as a novel biomarker in glioma: Evidence from meta-analysis, clinical validation and experimental investigations. Oncotarget 7: 33994–34010.Search in Google Scholar
Chuang HY, Su YK, Liu HW, Chen CH, Chiu SC, et al. (2019) Preclinical evidence of STAT3 inhibitor pacritinib overcoming temozolomide resistance via downregulating miR-21-enriched exosomes from M2 glioblastoma-associated macrophages. J Clin Med 8: 959.Search in Google Scholar
Hoshino A, Costa-Silva B, Shen TL, Rodrigues G, Hashimoto A, et al. (2015) Tumour exosome integrins determine organotropic metastasis. Nature 527: 329–335.Search in Google Scholar
Tian M, Yang L, Zhao Z, Li J, Wang L, et al. (2024) TIPE drives a cancer stem-like phenotype by promoting glycolysis via PKM2/HIF-1α axis in melanoma. Elife 13.Search in Google Scholar
Yang E, Wang X, Gong Z, Yu M, Wu H, et al. (2020) Exo-some-mediated metabolic reprogramming: the emerging role in tumor microenvironment remodeling and its influence on cancer progression. Signal Transduct Target Ther 5: 242.Search in Google Scholar
Li J, Wang A, Guo H, Zheng W, Chen R, et al. (2025) Exosomes: innovative biomarkers leading the charge in non-invasive cancer diagnostics. Theranostics 15: 5277–5311.Search in Google Scholar
Mehta MJ, Shin D, Park HS, An JS, Lim S Il, et al. (2025) Exosome-Based Theranostic for Gastrointestinal Cancer: Advances in Biomarker Discovery and Therapeutic Engineering. Small Methods 2402058.Search in Google Scholar
Xiao Q, Tan M, Yan G, Peng L (2025) Revolutionizing lung cancer treatment: harnessing exosomes as early diagnostic biomarkers, therapeutics and nano-delivery platforms. J Nanobiotechnology 23: 1–19.Search in Google Scholar
Wang F, Wang C, Chen S, Wei C, Ji J, et al. (2025) Identification of blood-derived exosomal tumor RNA signatures as noninvasive diagnostic biomarkers for multi-cancer: a multi-phase, multi-center study. Mol Cancer 24: 1–21.Search in Google Scholar
Chatterjee M, Nag S, Gupta S, Mukherjee T, Shankar P, et al. (2025) MicroRNAs in lung cancer: their role in tumor progression, biomarkers, diagnostic, prognostic, and therapeutic relevance. Discov Oncol 16: 1–23.Search in Google Scholar
Wang S, He W, Wang C (2016) MiR-23a Regulates the Vasculogenesis of Coronary Artery Disease by Targeting Epidermal Growth Factor Receptor. Cardiovasc Ther 34: 199–208.Search in Google Scholar
Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, et al. (2015) Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature 523: 177–182.Search in Google Scholar
Zhang Z, Yin J, Lu C, Wei Y, Zeng A, et al. (2019) Exosomal transfer of long non-coding RNA SBF2-AS1 enhances chemoresistance to temozolomide in glioblastoma. J Exp Clin Cancer Res 38: 1–16.Search in Google Scholar
Li Z, Yanfang W, Li J, Jiang P, Peng T, et al. (2018) Tumor-released exosomal circular RNA PDE8A promotes invasive growth via the miR-338/MACC1/MET pathway in pancreatic cancer. Cancer Lett 432: 237–250.Search in Google Scholar
Qiu A, Luo Z, Liu X, Hou X, Xiao Y, et al. (2025) Exosomes: A Promising Tool for Liquid Biopsy in Prostate Cancer. http://www.xiahepublishing.com/ 4: 53–60.Search in Google Scholar
Biomolecules B, Shi Y, Pan Z, Duan J, Wang Z, et al. (2025) Multi-omics reveals that ST6GAL1 promotes colorectal cancer progression through LGALS3BP sialylation. Biomol Biomed.Search in Google Scholar
Ilamathi HS, El Andaloussi S, Wiklander OPB (2025) Targeted Tumor Delivery Using Extracellular Vesicles. Methods in Pharmacology and Toxicology. Humana, New York, NY: 125–153.Search in Google Scholar
Capone E, Iacobelli S, Sala G (2021) Role of galectin 3 binding protein in cancer progression: a potential novel therapeutic target. J Transl Med 19: 1–18.Search in Google Scholar
Voglstaetter M, Thomsen AR, Nouvel J, Koch A, Jank P, et al. (2019) Tspan8 is expressed in breast cancer and regulates E-cadherin/catenin signalling and metastasis accompanied by increased circulating extracellular vesicles. J Pathol 248: 421–437.Search in Google Scholar
Talukdar S, Chang Z, Winterhoff B, Starr TK (2021) Single-Cell RNA Sequencing of Ovarian Cancer: Promises and Challenges. Advances in Experimental Medicine and Biology. Springer, Cham: 113–123.Search in Google Scholar
Shi J, Zhang Y, Fan Y, Liu Y, Yang M (2024) Recent advances in droplet-based microfluidics in liquid biopsy for cancer diagnosis. Droplet 3: e92.Search in Google Scholar
Ko J, Wang Y, Sheng K, Weitz DA, Weissleder R (2021) Sequencing-Based Protein Analysis of Single Extracellular Vesicles. ACS Nano 15: 5631–5638.Search in Google Scholar
Mizenko RR, Brostoff T, Rojalin T, Koster HJ, Swindell HS, et al. (2021) Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers. J Nanobiotechnology 19: 1–18.Search in Google Scholar
Home - ClinicalTrials.gov https://clinicaltrials.gov/ accessed 9 May 2025.Search in Google Scholar
Geng JX, Lu YF, Zhou JN, Huang B, Qin Y (2025) Exosome technology: A novel and effective drug delivery system in the field of cancer therapy. World J Gastrointest Oncol 17: 101857.Search in Google Scholar
Ma C, Tang W, Wang J, Yang S, Hou J, et al. (2025) Application of engineered exosomes in tumor therapy. Am J Transl Res 17: 736.Search in Google Scholar
Nguyen LD, Sengupta S, Cho KI, Floru A, George RE, et al. (2025) A drug that induces the microRNA miR-124 enables differentiation of retinoic acid–resistant neuroblastoma cells. Sci Signal 18.Search in Google Scholar
Yueh PF, Chiang IT, Weng YS, Liu YC, Wong RCB, et al. (2025) Innovative dual-gene delivery platform using miR-124 and PD-1 via umbilical cord mesenchymal stem cells and exosome for glioblastoma therapy. J Exp Clin Cancer Res 44: 1–17.Search in Google Scholar
Lentsch E, Li L, Pfeffer S, Ekici AB, Taher L, et al. (2019) CRISPR/Cas9-mediated knock-out of krasG12D mutated pancreatic cancer cell lines. Int J Mol Sci 20: 5706.Search in Google Scholar
Kundu S, Guo J, Islam MS, Rohokale R, Jaiswal M, et al. (2025) A New Strategy to Functionalize Exosomes via Enzymatic Engineering of Surface Glycans and its Application to Profile Exosomal Glycans and Endocytosis. Adv Sci 2415942.Search in Google Scholar
Chao T, Zhao J, Gao R, Wang H, Guo J, et al. (2025) Exo-some surface modification and functionalization: a narrative review of emerging technologies and their application potential in precision medicine. Adv Technol Neurosci 2: 27–33.Search in Google Scholar
Lopes J, Lopes D, Motallebi M, Ye M, Xue Y, et al. (2025) Biomembrane-coated nanosystems as next-generation delivery systems for the treatment of gastrointestinal cancers. Bioeng Transl Med e70006.Search in Google Scholar
Spada A, Gerber-Lemaire S (2025) Surface Functionalization of Nanocarriers with Anti-EGFR Ligands for Cancer Active Targeting. Nanomaterials 15: 158.Search in Google Scholar
Koneru T, McCord E, Pawar S, Tatiparti K, Sau S, et al. (2021) Transferrin: Biology and Use in Receptor-Targeted Nanotherapy of Gliomas. ACS Omega 6: 8727–8733.Search in Google Scholar
Hazrati A, Mirsanei Z, Heidari N, Malekpour K, Rahmani-Kukia N, et al. (2023) The potential application of encapsulated exosomes: A new approach to increase exosomes therapeutic efficacy. Biomed Pharmacother 162: 114615.Search in Google Scholar
Oskouie MN, Aghili Moghaddam NS, Butler AE, Zamani P, Sahebkar A (2019) Therapeutic use of curcumin-encapsulated and curcumin-primed exosomes. J Cell Physiol 234: 8182–8191.Search in Google Scholar
Kim MS, Haney MJ, Zhao Y, Mahajan V, Deygen I, et al. (2016) Development of exosome-encapsulated paclitaxel to overcome MDR in cancer cells. Nanomedicine Nano-technology, Biol Med 12: 655–664.Search in Google Scholar
Zheng W, Zhu T, Tang L, Li Z, Jiang G, et al. (2023) Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer. J Transl Med 21: 1–17.Search in Google Scholar
Alkhouri N, Gawrieh S (2021) A perspective on RNA interference-based therapeutics for metabolic liver diseases. Expert Opin Investig Drugs 30: 237–244.Search in Google Scholar
Ahmad MU, Liem IK, Wanandi SI (2024) Extracellular Vesicles from human umbilical cord Mesenchymal Stem Cells: a potential delivery system for RNA interference-based therapeutics in cancer. ExRNA.Search in Google Scholar
Leng Q, Woodle MC, Mixson AJ (2017) Targeted Delivery of siRNA Therapeutics to Malignant Tumors. J Drug Deliv 2017: 1–22.Search in Google Scholar
Tiwari P, Prakash Shukla R, Yadav K, Sharma M, Kumar Bakshi A, et al. (2025) YIGSR Functionalized Hybrid Exosomes Spatially Target Dasatinib to Laminin Receptors for Precision Therapy in Breast Cancer. Adv Healthc Mater 14: 2402673.Search in Google Scholar
Gharehchelou B, Mehrarya M, Sefidbakht Y, Uskoković V, Suri F, et al. (2025) Mesenchymal stem cell-derived exo-some and liposome hybrids as transfection nanocarriers of Cas9-GFP plasmid to HEK293T cells. PLoS One 20: e0315168.Search in Google Scholar
Soltanmohammadi F, Gharehbaba AM, Zangi AR, Soltanmohammadi F, Gharehbaba AM, et al. (2024) Current knowledge of hybrid nanoplatforms composed of exosomes and organic/inorganic nanoparticles for disease treatment and cell/tissue imaging. Biomed Pharmacother 178: 117248.Search in Google Scholar
Li Y, Cai Z, Wang Z, Zhu S, Liu W, et al. (2024) Construction of biomimetic hybrid nanovesicles based on M1 macrophage-derived exosomes for therapy of cancer. Chinese Chem Lett 36: 109942.Search in Google Scholar
Zhang F, Zhang Z, Yang W, Peng Z, Sun J, et al. (2024) Engineering Autologous Cell-Derived Exosomes to Boost Melanoma-Targeted Radio-Immunotherapy by Cascade cGAS-STING Pathway Activation. Small 21: 2408769.Search in Google Scholar
Nakamura T, Sato T, Endo R, Sasaki S, Takahashi N, et al. (2021) STING agonist loaded lipid nanoparticles overcome anti-PD-1 resistance in melanoma lung metastasis via NK cell activation. J Immunother Cancer 9: e002852.Search in Google Scholar
Su W, Tan M, Wang Z, Zhang J, Huang W, et al. (2023) Targeted Degradation of PD-L1 and Activation of the STING Pathway by Carbon-Dot-Based PROTACs for Cancer Immunotherapy. Angew Chemie - Int Ed 62: e202218128.Search in Google Scholar
Lee DH, Yun DW, Kim YH, Im GB, Hyun J, et al. (2023) Various Three-Dimensional Culture Methods and Cell Types for Exosome Production. Tissue Eng Regen Med 20: 621–635.Search in Google Scholar
Jafari D, Malih S, Eini M, Jafari R, Gholipourmalekabadi M, et al. (2020) Improvement, scaling-up, and downstream analysis of exosome production. Crit Rev Biotechnol 40: 1098–1112.Search in Google Scholar
Lee J (2024) Trends in Developing Extracellular Vesicle-Based Therapeutics. Brain Tumor Res Treat 12: 153.Search in Google Scholar
Ludwig N, Whiteside TL, Reichert TE (2019) Challenges in exosome isolation and analysis in health and disease. Int J Mol Sci 20: 4684.Search in Google Scholar
Wang CK, Tsai TH, Lee CH (2024) Regulation of exosomes as biologic medicines: Regulatory challenges faced in exosome development and manufacturing processes. Clin Transl Sci 17: e13904.Search in Google Scholar
Tzng E, Bayardo N, Yang PC (2023) Current challenges surrounding exosome treatments. Extracell Vesicle 2: 100023.Search in Google Scholar
Welsh JA, Goberdhan DCI, O’Driscoll L, Buzas EI, Blenkiron C, et al. (2024) Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J Extracell Vesicles 13: e12404.Search in Google Scholar
Song F, Wang C, Wang C, Wang J, Wu Y, et al. (2022) Multi-Phenotypic Exosome Secretion Profiling Micro-fluidic Platform for Exploring Single-Cell Heterogeneity. Small Methods 6: 2200717.Search in Google Scholar
Zeng H, Guo S, Ren X, Wu Z, Liu S, et al. (2023) Current Strategies for Exosome Cargo Loading and Targeting Delivery. Cells 12: 1416.Search in Google Scholar
Liang Y, Iqbal Z, Wang J, Xu L, Xu X, et al. (2022) Cell-derived extracellular vesicles for CRISPR/Cas9 delivery: engineering strategies for cargo packaging and loading. Bio-mater Sci 10: 4095–4106.Search in Google Scholar
Bhardwaj A, Tomar P, Nain V (2024) Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights. Eurobiotech J 8: 213–229.Search in Google Scholar
Nazari-Shafti TZ, Neuber S, Duran AG, Exarchos V, Beez CM, et al. (2020) Mirna profiles of extracellular vesicles secreted by mesenchymal stromal cells—can they predict potential off-target effects? Biomolecules 10: 1–50.Search in Google Scholar
Edelmann MJ, Kima PE (2022) Current Understanding of Extracellular Vesicle Homing/Tropism. Zoonoses (Ireland) 2: 14.Search in Google Scholar
Wei Z, Chen Z, Zhao Y, Fan F, Xiong W, et al. (2021) Mononuclear phagocyte system blockade using extracellular vesicles modified with CD47 on membrane surface for myocardial infarction reperfusion injury treatment. Biomaterials 275: 121000.Search in Google Scholar
Antes TJ, Middleton RC, Luther KM, Ijichi T, Peck KA, et al. (2018) Targeting extracellular vesicles to injured tissue using membrane cloaking and surface display. J Nanobio-technology 16: 1–15.Search in Google Scholar
Wang CK, Tsai TH, Lee CH (2024) Regulation of exosomes as biologic medicines: Regulatory challenges faced in exosome development and manufacturing processes. Clin Transl Sci 17: e13904.Search in Google Scholar
