1. bookTom 6 (2022): Zeszyt 3 (July 2022)
Informacje o czasopiśmie
License
Format
Czasopismo
eISSN
2564-615X
Pierwsze wydanie
30 Jan 2017
Częstotliwość wydawania
4 razy w roku
Języki
Angielski
access type Otwarty dostęp

Capsanthin induces death in human prostate cancer cell lines by inducing DNA damage

Data publikacji: 18 Jul 2022
Tom & Zeszyt: Tom 6 (2022) - Zeszyt 3 (July 2022)
Zakres stron: 99 - 104
Informacje o czasopiśmie
License
Format
Czasopismo
eISSN
2564-615X
Pierwsze wydanie
30 Jan 2017
Częstotliwość wydawania
4 razy w roku
Języki
Angielski
Abstract

There is a relationship between a person’s diet and the development and prevention of some cancers. Carotenoids are found as various natural pigments in many fruits and vegetables. Studies on carotenoids and their potential roles in carcinogenesis are increasing in importance day by day. In this study, we aimed to determine the cytotoxic and genotoxic effects of capsanthin, a carotenoid compound, in human prostate cancer cell lines.

After different concentrations of capsanthin were applied to human prostate cancer cell lines (LNCaP and PC-3), the effects of the compound on cell viability were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. The single-cell gel electrophoresis (Comet) assay was then used to reveal the genotoxic effects of probable cytotoxic dosages on cell DNA. After the treatments, apoptotic cell death levels were determined by Tunel staining. At high concentrations, capsanthin dramatically reduced PC-3 and LNCaP cell viability (p<0.05). In addition, capsanthin caused DNA damage and apoptotic cell death in the prostate cancer cells. The results show that capsanthin reduces cell viability by causing genotoxicity in prostate cancer cells.

Keywords

1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin 2021; 71: 209-24910.3322/caac.2166033538338 Search in Google Scholar

2. Wild CP, Weiderpass E, Stewart BW. World cancer report: cancer research for cancer prevention. Lyon, France: IARC Press; 2020 Search in Google Scholar

3. Matsushita M, Fujita K, Nonomura N. Influence of Diet and Nutrition on Prostate Cancer. Int J Mol Sci 2020; 21:10.3390/ijms21041447707309532093338 Search in Google Scholar

4. Rebbeck TR. Prostate Cancer Genetics: Variation by Race, Ethnicity, and Geography. Semin Radiat Oncol 2017; 27: 3-1010.1016/j.semradonc.2016.08.002517520827986209 Search in Google Scholar

5. Azzouni F, Mohler J. Role of 5alpha-reductase inhibitors in prostate cancer prevention and treatment. Urology 2012; 79: 1197-120510.1016/j.urology.2012.01.02422446342 Search in Google Scholar

6. Bonde Miranda T, Garmo H, Stattin P, Robinson D. 5alpha-Reductase Inhibitors and Risk of Prostate Cancer Death. J Urol 2020; 204: 714-71910.1097/JU.000000000000103832243243 Search in Google Scholar

7. Kallifatidis G, Hoy JJ, Lokeshwar BL. Bioactive natural products for chemoprevention and treatment of castration-resistant prostate cancer. Semin Cancer Biol 2016; 40-41: 160-16910.1016/j.semcancer.2016.06.003506719527370570 Search in Google Scholar

8. Šeregelj V, Vulić J, Ćetković G, Čanadanovć-Brunet J, Tumbas Šaponjac V, Stajčić S. Chapter 9 - Natural bioactive compounds in carrot waste for food applications and health benefits. In: Atta ur R ed, Studies in Natural Products Chemistry: Elsevier; 2020: 307-34410.1016/B978-0-12-819483-6.00009-6 Search in Google Scholar

9. Rao AV, Rao LG. Carotenoids and human health. Pharmacol Res 2007; 55: 207-21610.1016/j.phrs.2007.01.01217349800 Search in Google Scholar

10. Xavier AA, Perez-Galvez A. Carotenoids as a Source of Antioxidants in the Diet. Subcell Biochem 2016; 79: 359-37510.1007/978-3-319-39126-7_1427485230 Search in Google Scholar

11. Perez-Galvez A, Martin HD, Sies H, Stahl W. Incorporation of carotenoids from paprika oleoresin into human chylomicrons. Br J Nutr 2003; 89: 787-79310.1079/BJN200384212828795 Search in Google Scholar

12. Terlikowska KM, Dobrzycka B, Kinalski M, Terlikowski SJ. Serum Concentrations of Carotenoids and Fat-Soluble Vitamins in Relation to Nutritional Status of Patients with Ovarian Cancer. Nutr Cancer 2021; 73: 1480-148810.1080/01635581.2020.1801779 Search in Google Scholar

13. Huang J, Weinstein SJ, Yu K, Mannisto S, Albanes D. Serum Beta Carotene and Overall and Cause-Specific Mortality. Circ Res 2018; 123: 1339-134910.1161/CIRCRESAHA.118.313409 Search in Google Scholar

14. Giuffrida D, Dugo P, Torre G, Bignardi C, Cavazza A, Corradini C, Dugo G. Characterization of 12 Capsicum varieties by evaluation of their carotenoid profile and pungency determination. Food Chem 2013; 140: 794-80210.1016/j.foodchem.2012.09.060 Search in Google Scholar

15. Deli J, Matus Z, Tóth G. Carotenoid Composition in the Fruits of Capsicum annuum Cv. Szentesi Kosszarvú during Ripening. Journal of Agricultural and Food Chemistry 1996; 44: 711-71610.1021/jf950354n Search in Google Scholar

16. Pérez-Gálvez A, Mínguez-Mosquera MI. Structure−Reactivity Relationship in the Oxidation of Carotenoid Pigments of the Pepper (Capsicum annuum L.). Journal of Agricultural and Food Chemistry 2001; 49: 4864-486910.1021/jf010547c Search in Google Scholar

17. Murakami A, Nakashima M, Koshiba T, Maoka T, Nishino H, Yano M, Sumida T, Kyung Kim O, Koshimizu K, Ohigashi H. Modifying effects of carotenoids on superoxide and nitric oxide generation from stimulated leukocytes. Cancer Letters 2000; 149: 115-12310.1016/S0304-3835(99)00351-1 Search in Google Scholar

18. Aizawa K, Inakuma T. Dietary capsanthin, the main carotenoid in paprika (Capsicum annuum), alters plasma high-density lipoprotein-cholesterol levels and hepatic gene expression in rats. Br J Nutr 2009; 102: 1760-176610.1017/S000711450999130919646292 Search in Google Scholar

19. Agarwal S, Rao AV. Tomato lycopene and its role in human health and chronic diseases. CMAJ 2000; 163: 739-744 Search in Google Scholar

20. Li X, Xu J. Dietary and circulating lycopene and stroke risk: a meta-analysis of prospective studies. Sci Rep 2014; 4: 503110.1038/srep05031538137624848940 Search in Google Scholar

21. Zigangirova NA, Morgunova EY, Fedina ED, Shevyagina NV, Borovaya TG, Zhukhovitsky VG, Kyle NH, Petyaev IM. Lycopene Inhibits Propagation of Chlamydia Infection. Scientifica (Cairo) 2017; 2017: 147862510.1155/2017/1478625560262128948060 Search in Google Scholar

22. Levy J, Bosin E, Feldman B, Giat Y, Miinster A, Danilenko M, Sharoni Y. Lycopene is a more potent inhibitor of human cancer cell proliferation than either alpha-carotene or beta-carotene. Nutr Cancer 1995; 24: 257-26610.1080/016355895095144158610045 Search in Google Scholar

23. Duran-Cabral M, Fernandez-Jalao I, Estevez-Santiago R, Olmedilla Alonso B. Assessment of individual carotenoid and vitamin A dietary intake in overweight and obese Dominican subjects. Nutr Hosp 2017; 34: 407-41510.20960/nh.689 Search in Google Scholar

24. Cheng HM, Koutsidis G, Lodge JK, Ashor AW, Siervo M, Lara J. Lycopene and tomato and risk of cardiovascular diseases: A systematic review and meta-analysis of epidemiological evidence. Crit Rev Food Sci Nutr 2019; 59: 141-15810.1080/10408398.2017.1362630 Search in Google Scholar

25. Anand P, Kunnumakkara AB, Sundaram C, Harikumar KB, Tharakan ST, Lai OS, Sung B, Aggarwal BB. Cancer is a preventable disease that requires major lifestyle changes. Pharm Res 2008; 25: 2097-211610.1007/s11095-008-9661-9 Search in Google Scholar

26. Celebioglu HU, Erden Y, Hamurcu F, Taslimi P, Şentürk OS, Özmen ÜÖ, Tuzun B, Gulçin İ. Cytotoxic effects, carbonic anhydrase isoenzymes, α-glycosidase and acetylcholinesterase inhibitory properties, and molecular docking studies of heteroatom-containing sulfonyl hydrazone derivatives. Journal of Biomolecular Structure and Dynamics 2021; 39: 5539-555010.1080/07391102.2020.1792345 Search in Google Scholar

27. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods 1983; 65: 55-6310.1016/0022-1759(83)90303-4 Search in Google Scholar

28. Pu X, Wang Z, Klaunig JE. Alkaline Comet Assay for Assessing DNA Damage in Individual Cells. Curr Protoc Toxicol 2015; 65: 3 12 11-13 12 1110.1002/0471140856.tx0312s65 Search in Google Scholar

29. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184-19110.1016/0014-4827(88)90265-0 Search in Google Scholar

30. Erden Y. Sour black mulberry (Morus nigra L.) causes cell death by decreasing mutant p53 expression in HT-29 human colon cancer cells. Food Bioscience 2021; 42: 10111310.1016/j.fbio.2021.101113 Search in Google Scholar

31. Molnar J, Gyemant N, Mucsi I, Molnar A, Szabo M, Kortvelyesi T, Varga A, Molnar P, Toth G. Modulation of multidrug resistance and apoptosis of cancer cells by selected carotenoids. In Vivo 2004; 18: 237-244 Search in Google Scholar

32. Zhang X, Zhao WE, Hu L, Zhao L, Huang J. Carotenoids inhibit proliferation and regulate expression of peroxi-some proliferators-activated receptor gamma (PPARgamma) in K562 cancer cells. Arch Biochem Biophys 2011; 512: 96-10610.1016/j.abb.2011.05.00421620794 Search in Google Scholar

33. Eliassen AH, Liao X, Rosner B, Tamimi RM, Tworoger SS, Hankinson SE. Plasma carotenoids and risk of breast cancer over 20 y of follow-up. Am J Clin Nutr 2015; 101: 1197-120510.3945/ajcn.114.105080444181125877493 Search in Google Scholar

34. Cartmel B, Anderson C, Irwin ML, Harrigan M, Sanft T, Li F, Gellermann W, Ermakov IV, Ferrucci LM. Skin carotenoids are inversely associated with adiposity in breast cancer survivors. Nutr Res 2020; 79: 77-8610.1016/j.nutres.2020.05.012740955332650223 Search in Google Scholar

35. Veeramachaneni S, Wang XD. Carotenoids and lung cancer prevention. Front Biosci (Schol Ed) 2009; 1: 258-27410.2741/s2519482701 Search in Google Scholar

36. Nkondjock A, Ghadirian P. Dietary carotenoids and risk of colon cancer: case-control study. Int J Cancer 2004; 110: 110-11610.1002/ijc.2006615054875 Search in Google Scholar

37. Trejo-Solis C, Pedraza-Chaverri J, Torres-Ramos M, Jimenez-Farfan D, Cruz Salgado A, Serrano-Garcia N, Osorio-Rico L, Sotelo J. Multiple molecular and cellular mechanisms of action of lycopene in cancer inhibition. Evid Based Complement Alternat Med 2013; 2013: 70512110.1155/2013/705121373652523970935 Search in Google Scholar

Polecane artykuły z Trend MD

Zaplanuj zdalną konferencję ze Sciendo