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Influence of scarification method on seed germination of the terrestrial orchid Anacamptis laxiflora (Lam.)

Gwenaëlle Deconninck
Gwenaëlle Deconninck
 oraz 
Argyrios Gerakis
Argyrios Gerakis
   | 22 sty 2021
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Article Category: Research Article
Data publikacji: 22 sty 2021
Zakres stron: 15 - 23
DOI: https://doi.org/10.2478/ebtj-2021-0004
Słowa kluczowe
© 2021 Gwenaëlle Deconninck, Argyrios Gerakis, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Effect of time and three disinfection-plus-scarification methods (NaClO, CENTRIFUGE, and PRESOAK) on the proportion of germinated vessels of Anacamptis laxiflora 189 d after sowing. Markers are measured points (n = 16) and lines are fitted models.
Effect of time and three disinfection-plus-scarification methods (NaClO, CENTRIFUGE, and PRESOAK) on the proportion of germinated vessels of Anacamptis laxiflora 189 d after sowing. Markers are measured points (n = 16) and lines are fitted models.

Figure 2

Germinated seeds of Anacamptis laxiflora 189 d after sowing.
Germinated seeds of Anacamptis laxiflora 189 d after sowing.

Figure 3

Effect of three disinfection-plus-scarification methods on the proportion of germinated vessels at 41 versus 189 d. At 189 d, the parameter estimates for the odds-ratios were not statistically significant for CENTRIFUGE (p>|t| = 0.720) and PRESOAK (p>|t| = 0.068) compared to the reference method, whereas at 41 d the parameter estimate was statistically significant for CENTRIFUGE (p>|t| < 0.001). Centrifugation seems to severely delay germination, although given enough time the proportion of germinated vessels catches up.
Effect of three disinfection-plus-scarification methods on the proportion of germinated vessels at 41 versus 189 d. At 189 d, the parameter estimates for the odds-ratios were not statistically significant for CENTRIFUGE (p>|t| = 0.720) and PRESOAK (p>|t| = 0.068) compared to the reference method, whereas at 41 d the parameter estimate was statistically significant for CENTRIFUGE (p>|t| < 0.001). Centrifugation seems to severely delay germination, although given enough time the proportion of germinated vessels catches up.

Figure 4

Inverse prediction: The curves predict disinfection/scarification time for a given germination rate, according to fitted model.
Inverse prediction: The curves predict disinfection/scarification time for a given germination rate, according to fitted model.

j.ebtj-2021-0004.tab.004.w2aab3b7c12b1b6b1ab1b2b2b6Aa

TermNEstimateSEzp > |z|OR95% C.I.
Intercept (β0)144-3.88660.447-8.694<.0010.021[0.008 – 0.047]
Duration (β1)4320.10230.00910.790<.0011.108[1.089 – 1.130]
Centrifuge (β2)1440.19690.3630.5430.5871.218[0.598 – 2.492]
Presoak (β3)1440.99470.3732.6640.0082.704[1.315 – 5.709]

Logistic regression results and parameter estimates for log odds-ratios from Eq. [2]. The regressors are duration of soaking in NaClO and scarification method. The NaClO method was used as the reference

Model-LLDFχ2p > χ2
Difference142.213284.42<.001
Full145.08
Reduced287.29
R20.50
N432

Formula for modified “SM-organic” nutrient medium

IngredientsQuantity
Bottled water to make

Vikos® (Ioannina, Greece)

1 L
CaHPO499 mg

Equivalent to 75 mg Ca3(PO4)2 in the original formula

KH2PO475 mg
MgSO4.7H2O75 mg
Soluvit®

Fresenius Kabi, Uppsala, Sweden

10 mL
Amina-Fe

Humofert, Metamorfosi, Greece

0.92 mL

Equivalent to 0.5 mL Vaminolac® (Fresenius Kabi, Uppsala, Sweden) plus 10 mg FeSO4 in the original formula

Kinetin5 mg
Saccharose (sucrose)10 g
Activated charcoal1 g
Danish agar12 g
Pineapple juice25 mL
0.1 M H2SO4 for pH adjustment15–20 drops
Potato (Solanum tuber tuberosum)1 cm3 per culture vessel

Combinations of “dummy” or indicator variables that uniquely identify each scarification method for the purpose of logistic regression analysis. The NaClO method is specified as reference, in which case X2 = X3 = 0

VariableNaClOCENTRIFUGEPRESOAK
X2010
X3001
eISSN:
2564-615X
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Genetics, Biotechnology, Bioinformatics, other
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