<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The objective of this study was to present a comparative analysis of the antimicrobial activity of methanolic leaf extracts from seven <italic>Juniperus</italic> L. species, together with a tentative analysis of their total flavonoid and <italic>o-</italic>dihydroxyphenolic acids content. The chemical analyses were performed by colorimetric methods and the antimicrobial activity was assessed via broth microdilution. The studied extracts showed total content of <italic>o-</italic>dihydroxyphenols in the range of 26 to 34 µg of caffeic acid equivalent/mg DE and total flavonoid content of 13 to 24 µg of quercetin equivalent/mg DE. The sensitivity of Gram-positive bacteria to the studied extracts differed significantly with the following order of activity: <italic>J. sabina</italic> var. <italic>balkanensis > J. communis ‘</italic>Laxa’ <italic>> J. formosana</italic> > <italic>J. pinchotii > J. ashei > J. excelsa</italic> ≈ <italic>J. sibirica</italic>. Weak activity was observed for all extracts against Gram-negative bacteria and <italic>Candida</italic> strains. The analysis of the MBC/MIC ratio showed that the extracts exhibited bactericidal effect against Gram-negative bacteria (MBC/MIC≤4), while bactericidal or bacteriostatic action (MBC/MIC>4) was determined towards Gram-positive bacteria. Moreover, these extracts showed fungicidal (MFC/MIC≤4) or fungistatic effect (MFC/MIC>4). Best antibacterial activity was registered for the <italic>J. sabina</italic> var. <italic>balkanensis</italic> leaf extract. The multivariate data analyses were carried out by SIMCA 16 (v16.0.2) software. The hierarchical cluster analysis and principal component analysis, based on phytochemical and antimicrobial data, classified the studied juniper species into four groups: 1. <italic>J. ashei</italic>; 2. <italic>J. excelsa</italic>, <italic>J. sibirica</italic>; 3. <italic>J. sabina</italic> var. <italic>balkanensis</italic>, <italic>J. communis</italic> Laxa, <italic>J. formosana</italic>; 4. <italic>J. pinchotii.</italic></p>
</abstract>

The objective of this study was to present a comparative analysis of the antimicrobial activity of methanolic leaf extracts from seven Juniperus L. species, together with a tentative analysis of their total flavonoid and o-dihydroxyphenolic acids content. The chemical analyses were performed by colorimetric methods and the antimicrobial activity was assessed via broth microdilution. The studied extracts showed total content of o-dihydroxyphenols in the range of 26 to 34 µg of caffeic acid equivalent/mg DE and total flavonoid content of 13 to 24 µg of quercetin equivalent/mg DE. The sensitivity of Gram-positive bacteria to the studied extracts differed significantly with the following order of activity: J. sabina var. balkanensis > J. communis ‘Laxa’ > J. formosana > J. pinchotii > J. ashei > J. excelsa ≈ J. sibirica. Weak activity was observed for all extracts against Gram-negative bacteria and Candida strains. The analysis of the MBC/MIC ratio showed that the extracts exhibited bactericidal effect against Gram-negative bacteria (MBC/MIC≤4), while bactericidal or bacteriostatic action (MBC/MIC>4) was determined towards Gram-positive bacteria. Moreover, these extracts showed fungicidal (MFC/MIC≤4) or fungistatic effect (MFC/MIC>4). Best antibacterial activity was registered for the J. sabina var. balkanensis leaf extract. The multivariate data analyses were carried out by SIMCA 16 (v16.0.2) software. The hierarchical cluster analysis and principal component analysis, based on phytochemical and antimicrobial data, classified the studied juniper species into four groups: 1. J. ashei; 2. J. excelsa, J. sibirica; 3. J. sabina var. balkanensis, J. communis Laxa, J. formosana; 4. J. pinchotii.

Antimicrobial activity and chemical analyses of seven <italic>Juniperus</italic> L. species

Department of Pharmaceutical Microbiology

Department of Pharmacognosy with the Medicinal Plant Garden

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Antimicrobial activity and chemical analyses of seven  L. species

The objective of this study was to present a comparative analysis of the antimicrobial activity of methanolic leaf extracts from seven Juniperus L. species,...

Antimicrobial activity and chemical analyses of seven Juniperus L. species

Data publikacji: 25 gru 2023

Zakres stron: 236 - 241

Otrzymano: 24 wrz 2023

Przyjęty: 05 lis 2023

DOI: https://doi.org/10.2478/cipms-2023-0041

Słowa kluczowe
L., total flavonoid content, total o-dihydroxyphenols content, antimicrobial activity, multivariate component analysis

© 2023 Diana I. Ivanova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Antimicrobial activity and chemical analyses of seven Juniperus L. species

Data publikacji: 25 gru 2023

Zakres stron: 236 - 241

Otrzymano: 24 wrz 2023

Przyjęty: 05 lis 2023

DOI: https://doi.org/10.2478/cipms-2023-0041

Słowa kluczoweL., total flavonoid content, total o-dihydroxyphenols content, antimicrobial activity, multivariate component analysis

© 2023 Diana I. Ivanova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Słowa kluczowe
L., total flavonoid content, total o-dihydroxyphenols content, antimicrobial activity, multivariate component analysis