<abstract xmlns="http://www.w3.org/1999/xhtml">
<p>The study was conducted on spermatozoa of selected farm and free-living animal species, isolated <italic>post mortem</italic> from the tail of the epididymis, and stained with silver nitrate - AgNO<sc>3.</sc> The material was collected from pigs, goats, wild boar, and European roe deer. Twenty morphologically normal spermatozoa randomly selected from each animal and well visible under the microscope, were analysed. The following measurements were considered: head length, width, perimeter and area, acrosome area, mid-piece length, tail length, and overall sperm length. AgNO<sc>3</sc> staining differentiated the acrosomal (light hue) and distal (dark hue) part of the sperm head, and a light-hued mid-piece was visible within the sperm tail. Silver nitrate staining revealed species and variety-related differences, particularly in reference to the sperm head. Clear-cut differentiation within the head and tail area made it possible to perform detailed morphometric measurements of the spermatozoa.</p>
</abstract>

The study was conducted on spermatozoa of selected farm and free-living animal species, isolated post mortem from the tail of the epididymis, and stained with silver nitrate - AgNO3. The material was collected from pigs, goats, wild boar, and European roe deer. Twenty morphologically normal spermatozoa randomly selected from each animal and well visible under the microscope, were analysed. The following measurements were considered: head length, width, perimeter and area, acrosome area, mid-piece length, tail length, and overall sperm length. AgNO3 staining differentiated the acrosomal (light hue) and distal (dark hue) part of the sperm head, and a light-hued mid-piece was visible within the sperm tail. Silver nitrate staining revealed species and variety-related differences, particularly in reference to the sperm head. Clear-cut differentiation within the head and tail area made it possible to perform detailed morphometric measurements of the spermatozoa.

Use of Silver Nitrate for the Assessment of Sperm Measurements in Selected Farm and Free-Living Animal Species

Department of Animal Genetics and Horse Breeding, Institute of Bioengineering and Animal Breeding

Siedlce University of Natural Sciences and Humanities

Department of Breeding Methods and Poultry and Small Ruminant Breeding, Institute of Bioengineering and Animal Breeding

Department of Andrology and Reproductive Endocrinology

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The study was conducted on spermatozoa of selected farm and free-living animal species, isolated post mortem from the tail of the epididymis, and stained with...

Use of Silver Nitrate for the Assessment of Sperm Measurements in Selected Farm and Free-Living Animal Species

Data publikacji: 01 paź 2014

Zakres stron: 487 - 494

Otrzymano: 16 sty 2014

Przyjęty: 07 sie 2014

DOI: https://doi.org/10.2478/bvip-2014-0074

Słowa kluczowe
farm animals, free-living animals, sperm, acrosome, midpiece, silver nitrate

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Use of Silver Nitrate for the Assessment of Sperm Measurements in Selected Farm and Free-Living Animal Species

Data publikacji: 01 paź 2014

Zakres stron: 487 - 494

Otrzymano: 16 sty 2014

Przyjęty: 07 sie 2014

DOI: https://doi.org/10.2478/bvip-2014-0074

Słowa kluczowefarm animals, free-living animals, sperm, acrosome, midpiece, silver nitrate

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
farm animals, free-living animals, sperm, acrosome, midpiece, silver nitrate