<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Changes in selected blood lymphocyte subpopulations in dogs administered with a soluble parasitic antigen (SPA) derived from a supernatant of 18S RNA-A and 18S RNA-B <italic>Babesia canis </italic>cell culture were investigated. The studies included 20 dogs divided into three groups: group I (n=8) - comprised of dogs receiving SPA twice, at 3 week intervals; group II (n=5) - nonvaccinated control dogs, and group III (n=7) - dogs vaccinated twice with a commercial <italic>B. canis </italic>vaccine. Cytometric analysis revealed that vaccination with SPA derived from <italic>B. canis </italic>culture had similar effects to the vaccination with a commercial vaccine. The vaccination lowered the percentage of T lymphocytes (CD3+), T helper cells (CD4+), cytotoxic/supressor T cells (CD8+), B lymphocytes (CD21+), and MHC II lymphocytes in the blood in comparison to non-vaccinated dogs. Statistical analysis of the results demonstrated that mean values of the tested parameters at each stage of the study were similar in groups I and III and significantly higher in group II. The lowered level of the lymphocyte subpopulations in groups I and III persisted during the whole period of the study. The results presented that SPA has immunosuppressive effect in the first period after being administrated.</p></abstract>

Changes in selected blood lymphocyte subpopulations in dogs administered with a soluble parasitic antigen (SPA) derived from a supernatant of 18S RNA-A and 18S RNA-B Babesia canis cell culture were investigated. The studies included 20 dogs divided into three groups: group I (n=8) - comprised of dogs receiving SPA twice, at 3 week intervals; group II (n=5) - nonvaccinated control dogs, and group III (n=7) - dogs vaccinated twice with a commercial B. canis vaccine. Cytometric analysis revealed that vaccination with SPA derived from B. canis culture had similar effects to the vaccination with a commercial vaccine. The vaccination lowered the percentage of T lymphocytes (CD3+), T helper cells (CD4+), cytotoxic/supressor T cells (CD8+), B lymphocytes (CD21+), and MHC II lymphocytes in the blood in comparison to non-vaccinated dogs. Statistical analysis of the results demonstrated that mean values of the tested parameters at each stage of the study were similar in groups I and III and significantly higher in group II. The lowered level of the lymphocyte subpopulations in groups I and III persisted during the whole period of the study. The results presented that SPA has immunosuppressive effect in the first period after being administrated.

Quantitative Changes in Selected Lymphocyte Subpopulations after Administration of a Soluble Parasitic Antigen of Babesia Canis to Dogs

Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine

Edificio de Sanidad Animal Campus Universitario de Rabanales

Prof. F. Skubiszewski University School of Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Changes in selected blood lymphocyte subpopulations in dogs administered with a soluble parasitic antigen (SPA) derived from a supernatant of 18S RNA-A and 18S...

Quantitative Changes in Selected Lymphocyte Subpopulations after Administration of a Soluble Parasitic Antigen of Babesia Canis to Dogs

Data publikacji: 15 mar 2013

Zakres stron: 35 - 39

DOI: https://doi.org/10.2478/bvip-2013-0007

Słowa kluczowe
Babesia canis, soluble parasitic antigen, dogs, lymphocyte, flow cytometry

© 2013

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Quantitative Changes in Selected Lymphocyte Subpopulations after Administration of a Soluble Parasitic Antigen of Babesia Canis to Dogs

Data publikacji: 15 mar 2013

Zakres stron: 35 - 39

DOI: https://doi.org/10.2478/bvip-2013-0007

Słowa kluczoweBabesia canis, soluble parasitic antigen, dogs, lymphocyte, flow cytometry

© 2013

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
Babesia canis, soluble parasitic antigen, dogs, lymphocyte, flow cytometry