NodC -based evaluation of the occurrence of bacteria in nodules of Pisum sativum isolated on YEM agar
, , oraz
11 cze 2019
O artykule
Kategoria artykułu: Research Article
Data publikacji: 11 cze 2019
Zakres stron: 59 - 67
Otrzymano: 03 paź 2018
Przyjęty: 10 gru 2018
DOI: https://doi.org/10.2478/boku-2019-0005
Słowa kluczowe
© 2019 Anna Lenart-Boroń, Tadeusz Zając, Piotr Mateusz Boroń, Agnieszka Klimek-Kopyra, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
Detection of nodC gene in the studied bacterial isolatesTabelle 4_ Nachweis des nodC-Gens in den untersuchten Bakterienisolaten
| Isolate No. | Variant | Nodule | Number of isolates per variant | |
|---|---|---|---|---|
| 1 | Klif (uninoculated) | Large red | 1 | Yes |
| 2 | Klif (uninoculated) | Large red | 2 | No |
| 3 | Klif (uninoculated) | Small red | 1 | No |
| 4 | Klif (uninoculated) | Small red | 2 | No |
| 5 | Klif (uninoculated) | Large cream | 1 | Yes |
| 6 | Klif (uninoculated) | Small cream | 1 | Yes |
| 7 | Klif (uninoculated) | Small cream | 2 | No |
| 8 | Tarchalska (uninoculated) | Large red | 1 | Yes |
| 9 | Tarchalska (uninoculated) | Small red | 1 | Yes |
| 10 | Tarchalska (uninoculated) | Small red | 2 | No |
| 11 | Tarchalska (uninoculated) | Large cream | 1 | Yes |
| 12 | Tarchalska (uninoculated) | Small cream | 2 | Yes |
| 13 | Klif + IUNG | Large red | 1 | Yes |
| 14 | Klif + IUNG | Small red | 1 | Yes |
| 15 | Klif + IUNG | Large cream | 1 | Yes |
| 16 | Klif + IUNG | Small cream | 1 | No |
| 17 | Tarchalska + IUNG | Large red | 1 | Yes |
| 18 | Tarchalska + IUNG | Large red | 2 | No |
| 19 | Tarchalska + IUNG | Large red | 3 | No |
| 20 | Tarchalska + IUNG | Large red | 4 | No |
| 21 | Tarchalska + IUNG | Small red | 1 | No |
| 22 | Tarchalska + IUNG | Small red | 2 | No |
| 23 | Tarchalska + IUNG | Small red | 3 | No |
| 24 | Tarchalska/IUNG | Large cream | 1 | No |
| 25 | Tarchalska + IUNG | Large cream | 2 | No |
| 26 | Tarchalska + IUNG | Large cream | 3 | No |
| 27 | Tarchalska + IUNG | Small cream | 1 | No |
| 28 | Klif + Nitragine™ | Large red | 1 | No |
| 29 | Klif + Nitragine™ | Large red | 2 | No |
| 30 | Klif + Nitragine™ | Large red | 3 | No |
| 31 | Klif + Nitragine™ | Large red | 4 | No |
| 32 | Klif + Nitragine™ | Small red | 1 | Yes |
| 33 | Klif + Nitragine™ | Small red | 2 | No |
| 34 | Klif + Nitragine™ | Large cream | 1 | Yes |
| 35 | Klif + Nitragine™ | Small cream | 1 | Yes |
| 36 | Tarchalska/Nitragine™ | Large red | 1 | Yes |
| 37 | Tarchalska + Nitragine™ | Large red | 2 | No |
| 38 | Tarchalska + Nitragine™ | Small red | 1 | Yes |
| 39 | Tarchalska + Nitragine™ | Large cream | 1 | Yes |
| 40 | Tarchalska + Nitragine™ | Small cream | 1 | Yes |
| 41 | Tarchalska + Nitragine™ | Small cream | 2 | Yes |
| 42 | Tarchalska/Nitragine™ | Small cream | 3 | Yes |
| 43 | Tarchalska + Nitragine™ | Small cream | 4 | Yes |
| 44 | Nitragine™ | Nonapplicable | 1 | No |
| 45 | IUNG | Nonapplicable | 1 | Yes |
| 46 | Nonapplicable | 1 | No |
Oligonucleotides used for nodC amplificationTabelle 1_ Für die nodC-Amplifikation verwendete Oligonukleotide
| Primer | 5′–3′ nucleotide sequence |
|---|---|
| nodCF | AYGTHGTYGAYGACGGTTC |
| nodCFu | AYGTHGTYGAYGACGGITC |
| nodCF2 | AYGTHGTYGAYGACGGCTC |
| nodCF4 | AYGTHGTYGAYGACGGATC |
| nodCFn | AGGTGGTYGAYGACGGTTC |
| nodCI | CGYGACAGCCANTCKCTATTG |
Identification of bacteria colonizing pea nodulesTabelle 3_ Identifizierung der Bakterien, welche die Erbsenknöllchen besiedelten
| Variant | Bacteria species | Maximum identity (%) |
|---|---|---|
| IUNG | 99 | |
| NitragineTM | 99 | |
| Klif (uninoculated) | 99 | |
| Tarchalska (uninoculated) | 99 | |
| Tarchalska + IUNG | 99 | |
| Klif + NitragineTM | 98 | |
| Tarchalska + NitragineTM | 99 |
The mean numbers of colony forming units (CFUs) of bacteria colonizing pea nodules per one nodule depending on variants (pea varieties and inoculants)_Tabelle 2_ Die Durchschnittswerte koloniebildender Einheiten (KBE) von Bakterien, die Erbsenknollen besiedeln, pro Knöllchen in Abhängigkeit der Varianten (Erbsensorten und Inokulen)_
| Variant | Nodules | ||||
|---|---|---|---|---|---|
| Large cream | Small cream | Large red | Small red | Mean | |
| Klif (uninoculated) | 20.5 | 87.0 | 128.5 | 52.0 | |
| Tarchalska (uninoculated) | 6.0 | 110.0 | 157.5 | 36.0 | |
| Klif + IUNG | 84.0 | 97.5 | 310.0 | 212.0 | |
| Tarchalska + IUNG | 110.0 | 45.0 | 101.0 | 49.0 | |
| Klif + NitragineTM | 54.0 | 79.5 | 67.0 | 39.0 | |
| Tarchalska + NitragineTM | 45.0 | 54.0 | 32.0 | 53.0 | |