<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The Wingless/INT (WNT) signaling network has roles in renal cancer development. It was shown that the tumor-suppressor microRNA-124 (miR-124) is associated with the Wnt pathway. Thus, we aimed to measure miR-124 expression levels to evaluate whether it is a prognostic marker or a potential treatment strategy. Thirty tumor and 30 surrounding healthy kidney tissues from the same subjects diagnosed with renal cell carcinoma (RCC), were included in the study. The expression levels of miR-124 were measured with real-time polymerase chain reaction (qPCR) and determined by the 2<sup>–ΔΔCT</sup> method. The Statistical Package for Social Science (SPSS) version 22 program was used for statistical analyses and a <italic>p</italic> value of 0.05 was considered to be statistically significant. The expression levels of miR-124 was found to be about 3-fold lower in tumors than in healthy tissues (<italic>p</italic> 0.001) and decreased expression levels correlated with tumor stage, tumor diameter, body mass index (BMI) and neutrophil values (<italic>p</italic> 0.05). Our results showed that miR-124 expression levels are associated with RCC. MicroRNA-124 may be assessed as a biomarker in prognosis and the restoration of miR-124 expression might be effective in the treatment of RCC.</p></abstract>

The Wingless/INT (WNT) signaling network has roles in renal cancer development. It was shown that the tumor-suppressor microRNA-124 (miR-124) is associated with the Wnt pathway. Thus, we aimed to measure miR-124 expression levels to evaluate whether it is a prognostic marker or a potential treatment strategy. Thirty tumor and 30 surrounding healthy kidney tissues from the same subjects diagnosed with renal cell carcinoma (RCC), were included in the study. The expression levels of miR-124 were measured with real-time polymerase chain reaction (qPCR) and determined by the 2–ΔΔCT method. The Statistical Package for Social Science (SPSS) version 22 program was used for statistical analyses and a p value of 0.05 was considered to be statistically significant. The expression levels of miR-124 was found to be about 3-fold lower in tumors than in healthy tissues (p 0.001) and decreased expression levels correlated with tumor stage, tumor diameter, body mass index (BMI) and neutrophil values (p 0.05). Our results showed that miR-124 expression levels are associated with RCC. MicroRNA-124 may be assessed as a biomarker in prognosis and the restoration of miR-124 expression might be effective in the treatment of RCC.

Evaluation of microRNA-124 expression in renal cell carcinoma

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Evaluation of microRNA-124 expression in renal cell carcinoma"}

The Wingless/INT (WNT) signaling network has roles in renal cancer development. It was shown that the tumor-suppressor microRNA-124 (miR-124) is associated...

Evaluation of microRNA-124 expression in renal cell carcinoma

Article Category: Original article

Data publikacji: 23 mar 2021

Zakres stron: 73 - 78

DOI: https://doi.org/10.2478/bjmg-2020-0029

Słowa kluczowe
Expression, microRNA-124 (miR-124), Renal cell carcinoma (RCC)

© 2020 Çaykara B, Öztürk G, Alsaadoni H, Ötünçtemur A, Pençe S, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Evaluation of microRNA-124 expression in renal cell carcinoma

Article Category: Original article

Data publikacji: 23 mar 2021

Zakres stron: 73 - 78

DOI: https://doi.org/10.2478/bjmg-2020-0029

Słowa kluczoweExpression, microRNA-124 (miR-124), Renal cell carcinoma (RCC)

© 2020 Çaykara B, Öztürk G, Alsaadoni H, Ötünçtemur A, Pençe S, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
Expression, microRNA-124 (miR-124), Renal cell carcinoma (RCC)